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Recovery of inulin from Jerusalem artichoke (Helianthus tuberosus L.) in the small intestine of man

Published online by Cambridge University Press:  09 March 2007

Bach K. E. Knudsen  and
I. Hessov
Bach K. E. Knudsen
Affiliation:
Danish Institute of Animal Science, Department of Animal Physiology and Biochemistry, Research Centre Foulum, PO Box 39, DK-8830, Tjele, Denmark
I. Hessov
Affiliation:
Department of Surgery I, Aarhus University Hospital, Amtssygehuset, Tage-Hansens Gade 2, DK-8000, Aarhus C, Denmark
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Abstract

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The recovery of inulin, a naturally occurring β(2→l)-fructan isolated from Jerusalem artichoke (Helianthus tuberosus L.), in the small intestine of man was studied in ileostomy subjects. The ileostomists were given a low-dietary-fibre diet based on white wheat bread and virtually free of inulin, and the same diet with the addition of 10 g and 30 g inulin product respectively, and the recovery and mean transit time (MTT) of inulin were estimated by tracking inulin in ileal effluent. The recovery of inulin was approximately 87% at both ingestion levels. MTT was 4·9 (SE 0·6) h at an intake of 10 g inulin product decreasing to 3·4 (SE 0·3) h at an intake of 30 g Inulin product. A significant change in the fructose: glucose ratio of inulin from ingestion (4·1) to recovery in ileal effluent (4·5–4·7) and a lower recovery of the glucose residue than of the fructose residue of inulin indicate that the low-molecular-weight inulins are more sensitive to hydrolysis than the high-molecular-weight fragments. The loss of inulin during passage through the small intestine is presumably due to hydrolysis by either acids or enzymes and to microbial degradation by the microfiora permanently colonizing the distal small intestine. The concentrations of lactic acid (LA) and short-chain fatty acids (SCFA) in frequently collected ileal effluents on the control day were approximately 6 mmol/l and approximately 55 mmol/l respectively. During periods with inulin ingestion the concentration of LA increased to 18–26 mmol/l (P < 0·052), while the concentration of SCFA ran converse and decreased to 18–32 mmol/l (P < 0·023). The osmotic loads (68 and 204 mosmol/l) associated with the ingestion of inulin product caused minor malabsorption of low-molecular-weight sugars.

Keywords

InulinIleostomyRecoveryMan
Type
Inulin digestion in the small intestine
Information
British Journal of Nutrition , Volume 74 , Issue 1 , July 1995 , pp. 101 - 113
Copyright
Copyright © The Nutrition Society 1995

References

Archbold, H. K. (1940). Fructosans in the monocotyledons. A review. New Phytologist 39, 185–219.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1990). Official Methods of Analysis. Arlington, Virginia: Association of Official Analytical Chemists.Google Scholar
Knudsen, Bach K. E., Åman, P. & Eggum, B. O. (1987). Nutritive value of Danish-grown barley varieties. I. Carbohydrates and other major constituents. Journal of Cereal Science 6, 173–186.CrossRefGoogle Scholar
Knudsen, K. E. Bach, Jensen, B. B. & Hansen, I. (1993). Digestion of polysaccharides and other major components in the small and large intestine of pigs fed on diets consisting of oat fractions rich in β-D-glucan. British Journal of Nutrition 70, 537–556.CrossRefGoogle Scholar
Cummings, J. H. & Englyst, H. N. (1991). Measurement of starch fermentation in the human large intestine. Canadian Journal of Physiology and Pharmacology 69, 121–129.CrossRefGoogle ScholarPubMed
Cummings, J. H., Pomare, E. W., Branch, W. J., Naylor, C. P. E. & Macfarlane, G. T. (1987). Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 28, 1221–1227.CrossRefGoogle ScholarPubMed
De Bruyn, A. & Van Loo, J. (1991). The identification by 1H - and 13C NMR spectroscopy of sucrose, 1-kestose, and neokestose in mixtures present in plant extracts. Carbohydrate Research 212, 131–136.CrossRefGoogle Scholar
De Leenheer, L. & Hoebregs, H. (1994). Progress in the elucidation of the composition of chicory inulin. Starch 46, 193–196.Google Scholar
Deis, R. C. (1994). Adding bulk without adding sucrose. Cereal Foods World 2, 93–97.Google Scholar
Drasar, B. S. & Hill, M. J. (1974). The distribution of bacterial flora in the intestine. In Human Intestinal Flora, pp. 36–50 [Drasar, B. S. and Hill, M. J. editors]. London: Academic Press.Google Scholar
Englyst, H. N. & Cummings, J. H. (1985). Digestion of polysaccharides of some cereal foods in the human small intestine. American Journal of Clinical Nutrition 42, 778–787.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Cummings, J. H. (1986). Digestion of the carbohydrates of banana (Musa paradisiaca sapientum) in the human small intestine. American Journal of Clinical Nutrition 44, 42–50.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Cummings, J. H. (1987). Digestion of polysaccharides of potato in the small intestine of man. American Journal of Clinical Nutrition 45, 423–431.CrossRefGoogle ScholarPubMed
Englyst, H. N., Wiggins, H. S. & Cummings, J. H. (1982). Determination of non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107, 307–318.CrossRefGoogle ScholarPubMed
Finegold, S. M., Sutter, V. L., Boyle, J. D. & Shimada, K. (1970). The normal flora of ileostomy and transverse colostomy effluents. Journal of Infectious Diseases 122, 376–381.CrossRefGoogle ScholarPubMed
Fleming, S. E. & GrootWassink, J. W. D. (1979). Preparation of high-fructose syrup from the tubers of the Jerusalem artichoke (Helianthus tuberosus L.). CRC Critical Reviews in Food Science and Nutrition 12, 1–28.CrossRefGoogle ScholarPubMed
Fussell, R. J. & McCalley, D. V. (1987). Determination of volatile fatty acids (C2-C5) and lactic acid in silage by gas chromatography. Analyst 112, 1213–1216.CrossRefGoogle Scholar
Hidaka, H., Eida, T., Takizawa, T., Tokunaga, T. & Tashiro, Y. (1986). Effects of fructo-oligosaccharides on intestinal flora and human health. Bifidobacteria Microflora 5, 37–50.CrossRefGoogle Scholar
Hirst, E. L. (1957). Some aspects of the chemistry of the fructosans. Chemical Society Proceedings, 193–204.Google Scholar
Langkilde, A. M., Andersson, H., Schweizer, T. F. & Torsdottir, I. (1990). Nutrients excreted in ileostomy effluents after consumption of mixed diets with beans or potatoes. I. Minerals, protein, fat and energy. European Journal of Clinical Nutrition 44, 559–566.Google ScholarPubMed
Nilsson, U., Öste, R., Jägerstad, M. & Birkhed, D. (1988). Cereal fructans: in vitro and in vivo studies on availability in rats and humans. Journal of Nutrition 118, 1325–1330.CrossRefGoogle ScholarPubMed
Oku, T., Tokunga, T. & Hosoya, N. (1984). Nondigestibility of a new sweetener, “Neosugar”, in the rat. Journal of Nutrition 114, 1574–1581.Google Scholar
Quemener, B., Thibault, J.-F. & Coussement, P. (1994). Determination of inulin and oligofructose in food products, and integration in the AOAC method for measurement of total dietary fibre. Lebenmittel-Wissenschaft und-Technologie 21, 125–132.CrossRefGoogle Scholar
Roberfroid, M. (1993). Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. CRC Critical Reviews in Food Science and Nutrition 33, 103–148.CrossRefGoogle ScholarPubMed
Roberfroid, M., Gibson, G. R. & Delzenne, N. (1993). The biochemistry of oligofructose, a nondigestible fiber: an approach to calculate its caloric value. Nutrition Reviews 51, 137–146.CrossRefGoogle ScholarPubMed
Rumessen, J. J., Bodé, S., Hamberg, O. & Gudmand-Høyer, E. (1990). Fructans of Jerusalem artichokes: intestinal transport, absorption, fermentation, and influence on blood glucose, insulin, and C-peptide response in healthy subjects. American Journal of Clinical Nutrition 52, 675–681.CrossRefGoogle ScholarPubMed
Sandberg, A.-S., Ahderinne, R., Andersson, H., Hallgreen, B. & Hultén, L. (1983). The effect of citrus pectin on the absorption of nutrients in the small intestine. Human Nutrition: Clinical Nutrition 37C, 171–183.Google ScholarPubMed
Schweizer, T. F., Andersson, H., Langkilde, A. M., Reimann, S. & Torsdottir, I. (1990). Nutrients excreted in ileostomy effluents after consumption of mixed diets with beans or potatoes. II. Starch, dietary fibre and sugars. European Journal of Clinical Nutrition 44, 567–575.Google ScholarPubMed
Scott, R. W. (1979). Colorimetric determination of hexuronic acids in plant materials. Analytical Chemistry 51, 936–941.CrossRefGoogle Scholar
Snedecor, G. W. & Cochran, W. G. (1973). Statistical Methods. Ames: Iowa State University Press.Google Scholar
Stone-Dorshow, T. & Levitt, M. D. (1987). Gaseous response to ingestion of poorly absorbed fructo-oligosaccharide sweetener. American Journal of Clinical Nutrition 46, 61–65.CrossRefGoogle ScholarPubMed
Theander, O. & Åman, P. (1979). Studies on dietary fibre. 1. Analysis and chemical characterization of water-soluble and water-insoluble dietary fibres. Swedish Journal of Agricultural Research 9, 97–106.Google Scholar
Theander, O. & Westerlund, E. A. (1986). Studies on dietary fiber. 3. Improved procedures for analysis of dietary fiber. Journal of Agricultural and Food Chemistry 34, 330–336.CrossRefGoogle Scholar
Van Loo, J., Coussement, P., De Leenheer, L., Hoebergs, L. & Smits, G. (1995). On the presence of inulin and oligofructose as natural ingredients in the western diet. CRC Critical Reviews in Food Science and Nutrition (In the Press.)CrossRefGoogle ScholarPubMed
Wang, X. & Gibson, G. (1993). Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the large intestine. Journal of Applied Bacteriology 75, 373–380.CrossRefGoogle Scholar
Yamashita, K., Kawai, K. & Itakura, M. (1984). Effects of fructo-oligosaccharides on blood glucose and serum lipids in diabetic subjects. Nutrition Research 4, 961–966.CrossRefGoogle Scholar