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A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: relationship with intramuscular lipid formation

Published online by Cambridge University Press:  08 March 2007

O. Doran ,
S. K. Moule ,
G. A. Teye ,
F. M. Whittington ,
K. G. Hallett  and
J. D. Wood
O. Doran*
Affiliation:
University of BristolDepartment of Clinical Veterinary Science, Langford, Bristol BS40 5DUUK
S. K. Moule
Affiliation:
University WalkDepartment of Biochemistry, University of BristolUK
G. A. Teye
Affiliation:
University of BristolDepartment of Clinical Veterinary Science, Langford, Bristol BS40 5DUUK
F. M. Whittington
Affiliation:
University of BristolDepartment of Clinical Veterinary Science, Langford, Bristol BS40 5DUUK
K. G. Hallett
Affiliation:
University of BristolDepartment of Clinical Veterinary Science, Langford, Bristol BS40 5DUUK
J. D. Wood
Affiliation:
University of BristolDepartment of Clinical Veterinary Science, Langford, Bristol BS40 5DUUK
*
*Corresponding author: fax +44 (0)117 928 9582, email e.udovikova@bristol.ac.uk
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Abstract

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A reduced protein diet (RPD) is known to increase the level of intramuscular lipid in pig meat with a smaller effect on the amount of subcutaneous adipose tissue. This might be due to tissue-specific activation of the expression of lipogenic enzymes by the RPD. The present study investigated the effect of a RPD, containing palm kernel oil, soyabean oil or palm oil on the activity and expression of one of the major lipogenic enzymes, stearoyl-CoA desaturase (SCD) and on the level of total lipids and the fatty acid composition of muscle and subcutaneous adipose tissue in pigs. The RPD significantly increased SCD protein expression and activity in muscle but not in subcutaneous adipose tissue. The level of MUFA and total fatty acids in muscle was also elevated when the RPD was fed, with only small changes in subcutaneous adipose tissue. A positive significant correlation between SCD protein expression and total fatty acids in muscle was found. The results suggest that an increase in intramuscular but not subcutaneous adipose tissue fatty acids under the influence of a RPD is related to tissue-specific activation of SCD expression. It is suggested that the SCD isoform spectra in pig subcutaneous adipose tissue and muscle might be different.

Keywords

Stearoyl-CoA desaturaseIntramuscular lipidPig: Reduced protein diet
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 609 - 617
Copyright
Copyright © The Nutrition Society 2006

References

Adeola, O & Young, LG 1989 Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism. J Anim Sci 67, 664–673.CrossRefGoogle ScholarPubMed
Barber, MC, Ward, RJ, Richards, SEet al. 2000 vine adipose tissue monounsaturated fat content is correlated to depot-specific expression of the stearoyl-CoA desaturase gene. O. J Anim Sci 78, 62–68.CrossRefGoogle Scholar
Bene, H, Lasky, D & Ntambi, JM 2001 Cloning and characterization of the human stearoyl CoA desaturase gene promoter: transcriptional activation by sterol regulatory element binding protein and repression by polyunsaturated fatty acids and cholesterol. Biochem Biophys Res Commun 284, 1194–1198.CrossRefGoogle ScholarPubMed
Candek-Potokar, M, Monin, G & Zlender, B 2002 Pork quality, processing, and sensory characteristics of dry-cured hams as influenced by Duroc crossing and sex. J Anim Sci 80, 988–996.CrossRefGoogle ScholarPubMed
Chung, M, Ha, S, Jeong, Set al. 2000 loning and characterization of bovine stearoyl CoA desaturase l cDNA from adipose tissues. C. Biosci Biotechnol Biochem 64, 1526–1530.CrossRefGoogle Scholar
Clarke, R, Frost, C, Collins, R, Appleby, P & Peto, R 1997 Dietary lipids and blood cholesterol: quantitative meta-analysis of metabolic ward studies. Br Med J, 314, 112–117.CrossRefGoogle ScholarPubMed
Cohen, P, Miyazaki, M, Socci, NDet al. Role for stearoyl-CoA desaturase-1 in leptin- mediated weight loss. Science 2002 297, 240–243.CrossRefGoogle ScholarPubMed
Da, Costa N, NcGillivray, C, Bai, Q, Wood, JEvans, G &Chang, K-C 2004 Restriction of dietary energy and protein induces molecular changes in young porcine skeletal muscles. J Nutr 134, 2119–2199.Google Scholar
Daniel, ZC, Wynn, RJ, Salter, AM & Buttery, PJ 2004 Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. Anim Sci 82, 747–758.CrossRefGoogle ScholarPubMed
De Vol, DL, McKeith, FK, Bechtel, PJ, Novakofski, J, Shanks, RD & Carr, TR 1988 Variations in composition and palatability traits and relationships between muscle: characteristics and palatability in a random sample of pork carcasses. J Anim Sci 66, 385–395.CrossRefGoogle Scholar
Duckett, SK, Wagner, DG, Yates, LD, Dolezal, HG & May, SG 1993 Effects of time of feed on beef nutrient composition. J Anim Sci 71, 2079–2088.CrossRefGoogle ScholarPubMed
Ebong, PE, Owu, DU & Isong, EU 1999 Influence of palm oil (Elaesis guineensis).on health. Plant Foods Hum Nutr 53, 209–222.CrossRefGoogle ScholarPubMed
Eikelenboom, G & Hoving-Bolink, AHThe effect of intramuscular fat on eating quality of pork.In(Proceedings of the 40th International Congress of Meat Science and Technology), abstract no. S-IVB.30, 1–2The Hague, The Netherlands. 1994Google Scholar
Enoch, HG, Catala, A & Strittmatter, P 1976 Mechanism of rat microsomal stearoyl CoA desaturase. J Biol Chem 251, 5095–5103.CrossRefGoogle Scholar
Ferguson, LR, Philpott, M & Karunasinghe, N 2004 Dietary cancer and prevention using antimutagens. Toxicology 198, 147–159.CrossRefGoogle ScholarPubMed
Fernandez, X, Monin, G, Talmant, A, Mourot, J & Lebret, B 1999 Influence of intramuscular fat content on the quality of pig meat 1. Composition of the lipidic fraction and sensory characteristics on muscle Longissimus Lumborum. Meat Sci 53, 59–65.CrossRefGoogle Scholar
Gondret, F, Lebas, F & Bonneau, M 2000 Restricted feed intake during fattening reduces intramuscular lipid deposition without modifying muscle fiber characteristics in rabbits. J Nutr 130, 228–233.CrossRefGoogle ScholarPubMed
Gondret, F, Lebret, B 2002 Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein. J Anim Sci 80, 3184–3193.CrossRefGoogle ScholarPubMed
Hsieh, SL & Kuo, CM 2005 Stearoyl-CoA desaturase expression and fatty acid composition in milkfish (Chanos chanos) and grass carp (Ctenopharyngodon idella) during cold acclimation. Comp Biochem Physiol B Biochem Mol Biol 141, 95–101.CrossRefGoogle ScholarPubMed
Kaestner, KH, Ntambi, JM, Kelly, TJ & Lane, MD 1989 Differentiationinduced gene expression in 3T3-L1 preadipocytes. A second differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem 264, 14755–14761.CrossRefGoogle ScholarPubMed
Karlsson, A, Enfalt, A-C, Essen-Gustavsson, B, Lundstrom, K, Rydhmer, L & Stern, S 1993 Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs. J Anim Sci 71, 930–938.CrossRefGoogle ScholarPubMed
Kim, HJ, Miyazaki, M & Ntambi, JM 2002 Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism. J Lipid Res 43, 1750–1757.CrossRefGoogle ScholarPubMed
Kim, Y-C, Gomez, FE, Fox, BG & Ntambi, JM 2000 Differential regulation of the stearoyl-CoA desaturase genes by thiazolidinediones in 3T3-L1 adipocytes. J Lipid Res 41, 1310–1316.CrossRefGoogle ScholarPubMed
Kouba, M, Hermier, D & Le, Dividich J 1999 Influence of a high ambient temperature on stearoyl CoA-desaturase activity, lipogenesis, and lipid composition in the growing pig. Comp Biochem Physiol B Biochem Mol Biol 124, 7–13.CrossRefGoogle Scholar
Lefevre, P, Tripon, E, Plumelet, C, Douaire, M & Diot, C 2001 Effects of polyunsaturated fatty acids and clofibrate on chicken stearoyl-CoA desaturase I gene expression. Biochem Biophys Res Commun 280, 25–31.CrossRefGoogle Scholar
Maloney, CA, Gosby, AK, Phuyal, JL, Denyer, GS, Bryson, JM & Caterson, ID 2003 Site-specific changes in the expression of fat-partitioning genes in weanling rats exposed to a low-protein diet in utero. Obesity Res 11, 461–468.CrossRefGoogle ScholarPubMed
Mensink, RP & Katan, MB 1992 Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb 12, 911–919.CrossRefGoogle ScholarPubMed
Miyazaki, M, Jacobson, MJ, Man, WC et al. , 2003 Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. J Biol Chem 278, 33904–33911.CrossRefGoogle ScholarPubMed
Miyazaki, M & Ntambi, JM 2003 Role of stearoyl-coenzyme A desaturase in lipid metabolism. Prostaglandins Leukot Essent Fatty Acids 68, 113–121.CrossRefGoogle ScholarPubMed
Mourot, J & Hermier, D 2001 Lipids in monogastric meat (review article.). Reprod Nutr Dev 41, 109–118.CrossRefGoogle Scholar
Mourot, J & Kouba, M 1998 Lipogenic enzyme activities in muscles of growing Large White and Meishan pigs. Livest Prod Sci 5, 127–133.CrossRefGoogle Scholar
Mourot, J, Kouba, M & Peiniau, P 1995 Comparative study of in vitro lipogenesis in various adipose tissues in the growing domestic pig (Sus domesticus). Comp Biochem Physiol B Biochem Mol Biol 111, 379–384.CrossRefGoogle ScholarPubMed
Ntambi, JM, 1999 Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. J Lipid Res 40, 1549–1558.CrossRefGoogle ScholarPubMed
Ntambi, JM, Buhrow, SA, Kaestner, KH et al. , 1988 Differentiationinduced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoy-CoA desaturase. J Biol Chem 263, 17291–17300.CrossRefGoogle Scholar
Perez, JF, Gernat, AG & Murillo, JG 2000 Research notes: the effect of different levels of palm kernel meal in layer diets. Poult Sci 79, 77–79.CrossRefGoogle ScholarPubMed
Ren, J, Knorr, C, Gou, Y-M et al. , 2004 Characterization of five single nucleotide polymorphisms in the porcine stearoyl-CoA desaturase (SCD) gene. Anim Genet 35, 245–264.CrossRefGoogle ScholarPubMed
Sacks, FM & Katan, M 2002 Randomized clinical trials on the effects of dietary fat and carbohydrate on plasma lipoproteins and cardiovascular disease. Am J Med 113, Suppl. 9, 13S–24S.CrossRefGoogle ScholarPubMed
Schenkman, JB & Cinti, DL 1978 Preparation of microsomes with calcium. Methods Enzymol 52, 83–89.CrossRefGoogle ScholarPubMed
Sellier, P & Monin, G 1994 Genetics of pig meat quality: a review. J Muscle Foods 5, 187–194.CrossRefGoogle Scholar
Singh, K, Hartley, DG, McFadden, TB & Mackenzie, DDDietary fat regulates mammary stearoyl CoA desaturase expression and activity in lactating mice. J Dairy Res 2004 71, 1–6.CrossRefGoogle ScholarPubMed
Smith, RD, Kelly, CN, Fielding, BA et al. , 2003 Long-term monounsaturated fatty acid diets reduce platelet aggregation in healthy young subjects. Br J Nutr 90, 597–606.CrossRefGoogle ScholarPubMed
Smith, SB, Hively, TS, Cortese, GM et al. , 2002 Conjugated linoleic acid depresses the D9 desaturase index and stearoyl coenzyme A desaturase enzyme activity in porcine subcutaneous adipose tissue. J Anim Sci 80, 2110–2115.Google Scholar
Smith, SB, Mersmann, HJ, Smith, EO & Britain, KG 1999 Stearoyl-coenzyme A desaturase gene expression during growth in adipose tissue from obese and crossbred pigs. J Anim Sci 77, 1710–1716.CrossRefGoogle ScholarPubMed
Thiede, MA, Ozols, J & Strittmatter, PJ 1986 Construction and sequence of cDNA for rat liver stearoyl coenzyme A desaturase. J Biol Chem 261, 13230–13235.CrossRefGoogle Scholar
Van Barneveld, RJ 2003 Modern pork production – balancing efficient growth and feed conversion with product quality requirements and consumer demands. Asia Pac J Clin Nutr 12, Suppl., S31.Google Scholar
Ward, RJ, Travers, MT, Richards, ES, Vernon, RG, Buttery, PJ & Barber, MCStearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochem Biophys Acta 1998 1391, 145–156.CrossRefGoogle ScholarPubMed
Winder, WW, MacLean, PS, Lucas, JC, Fernley, JE & Trumble, GE 1995 Effect of fasting and refeeding of acetyl-CoA carboxylase in rat hindlimb muscle. J Appl Physiol 78, 578–582.CrossRefGoogle ScholarPubMed
Wolfram, G, 2003 Dietary fatty acids and coronary heart disease. Eur J Med Res 20, 321–324.Google Scholar
Wood, JD, Nute, GR, Richiardson, RI et al. , 2004 Effect of breed, diet and muscle on fat deposition and eating quality in pigs. Meat Sci 67, 651–667.CrossRefGoogle ScholarPubMed
Yang, A, Larsen, TW, Smith, SB & Tume, RKDelta9 desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids 1999 34, 971–978.CrossRefGoogle ScholarPubMed
Zhang, L, Ge, L, Parimoo, S, Stenn, K & Prouty, SM 1999 Human stearoyl- CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340, Part 1 255–264.CrossRefGoogle ScholarPubMed
Zheng, Y, Prouty, SM, Harmon, A, Sundberg, JP, Stenn, K & Parimoo, S 2001 Scd3 – a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genomics 71, 182–191.CrossRefGoogle ScholarPubMed