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The relationships between potassium intakes, transmural potential difference of the rumen epithelium and magnesium absorption in wethers

Published online by Cambridge University Press:  09 March 2007

S. Jittakhot ,
J. Th. Schonewille ,
H. S. Wouterse ,
C. Yuangklang  and
A. C. Beynen
S. Jittakhot
Affiliation:
Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, P. O. Box 80.152, 3508 TD Utrecht, The Netherlands
J. Th. Schonewille*
Affiliation:
Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, P. O. Box 80.152, 3508 TD Utrecht, The Netherlands
H. S. Wouterse
Affiliation:
Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, P. O. Box 80.152, 3508 TD Utrecht, The Netherlands
C. Yuangklang
Affiliation:
Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, P. O. Box 80.152, 3508 TD Utrecht, The Netherlands
A. C. Beynen
Affiliation:
Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, P. O. Box 80.152, 3508 TD Utrecht, The Netherlands
*
*Corresponding author: Dr J. Thomas Schonewille, fax +31 30 2531817, email j.schonewille@vet.uu.nl
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Abstract

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In vitro studies with isolated sheep rumen epithelium have shown that an increase in the lumen K concentration induces an increase in the transmural potential difference across the rumen epithelium (serosal side: positive), which is associated with a decrease in Mg transport. However, at lumen K concentrations >80 mmol/l, Mg transport across the epithelium became independent of the lumen K concentration. The present study was carried out to determine whether this observation also occurs in vivo. Four ruminally fistulated wethers were fed four rations supplemented with KHCO3 (15·7, 37·6, 59·4 or 77·4 g K/kg DM) in a 4×4 Latin square design. Increased K intakes significantly increased the rumen K concentration. For all data combined, Mg absorption expressed as % intake was negatively correlated with the rumen K concentration. However, apparent Mg absorption either expressed in absolute terms (g/d) or as % intake was not significantly affected when the dietary K concentration was increased from 59·4 to 77·4 g/kg DM. Rumen K concentration was inversely correlated with the transmural potential difference (blood side: positive) (Pearson's r −0·709; R2adj 0·468, P=0·002, n 16). It is concluded that in wethers apparent Mg absorption becomes independent of the dietary K concentration when the K concentration is >60 g/kg DM or equivalent to a postprandial rumen K concentration of about 125 mmol/l.

Keywords

Magnesium absorptionPotassium intakeTransmural potential differenceSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 2 , February 2004 , pp. 183 - 189
Copyright
Copyright © The Nutrition Society 2004

References

Association of Official Analytical Chemists (1984) Official Methods of Analysis, 14th ed. Arlington, VA: AOAC.Google Scholar
Axford, RFE, Hughes, A & Evans, RA (1982) Magnesium ammonium phosphate precipitation and its significance in sheep. Proc Nutr Soc 41, 85A.Google Scholar
Centraal Veevoederbureau (2003) Animal Feed Tables Book. Lelystad: CVB.Google Scholar
Dalley, DE, Isherwood, P, Sykes, AR & Robson, AB (1997) Effect of in vitro manipulation of pH on magnesium solubility in ruminal and caecal digesta in sheep. J Agric Sci 129, 107–111.CrossRefGoogle Scholar
Ferreira, BM, Harrison, FA, Keynes, RD & Nauss, AH (1966) Observations on the potential across the rumen of sheep. J Physiol 187, 615–630.CrossRefGoogle Scholar
Fisher, LJ, Dinn, N, Trait, RM & Shelford, JA (1994) Effect of level of dietary potassium on the absorption and excretion of calcium and magnesium by lactating cows. Can J Anim Sci 74, 503–509.Google Scholar
Fontenot, JP, Allen, VG, Bunce, GE & Goff, JP (1989) Factors influencing magnesium absorption and metabolism in ruminants. J Anim Sci 67, 3445–3455.CrossRefGoogle ScholarPubMed
Greene, LW, Fontenot, JP & Webb, KE Jr (1983 a) Effect of dietary potassium on absorption of magnesium and other macroelements in sheep fed different levels of magnesium. J Anim Sci 56, 1208–1213.CrossRefGoogle ScholarPubMed
Greene, LW, Webb, KE Jr & Fontenot, JP (1983 b) Effect of potassium level on site of absorption of magnesium and other macroelements in sheep. J Anim Sci 56, 1214–1221.CrossRefGoogle ScholarPubMed
Grings, EE & Males, JR (1987) Effects of potassium on macromineral absorption in sheep fed wheat straw-based diets. J Anim Sci 64, 872–879.CrossRefGoogle ScholarPubMed
International Dairy Federation (1986) IDF Standard 20A. Brussels: International Dairy Federation.Google Scholar
Jittakhot, S, Schonewille, JT, Wouterse, H, Yuangklang, C & Beynen, AC (2003) Apparent magnesium absorption in dry cows fed at three levels of potassium and two levels of magnesium intake. J Dairy Sci (In the Press).CrossRefGoogle Scholar
Kemp, A (1960) Hypomagnesaemia in milking cows; the response of serum magnesium to alteration in herbage composition resulting from potash and nitrogen dressing on pasture. Neth J Agric Sci 8, 281–303.Google Scholar
Khorasani, GR & Armstrong, DG (1990) Effects of sodium and potassium level on the absorption of magnesium and other macro-minerals in sheep. Livest Prod Sci 24, 223–235.CrossRefGoogle Scholar
Leonhard, S, Martens, H & Gabel, G (1989) New aspects of magnesium transport in ruminants. Acta Vet Scand 86, 146–151.Google ScholarPubMed
Leonhard-Marek, S & Martens, H (1996) Effects of potassium on magnesium transport across rumen epithelium. Am J Physiol 271, G1034–G1038.Google Scholar
McLean, AF, Buchan, W & Scott, D (1985) The effect of potassium and magnesium infusion on plasma magnesium concentration and magnesium balance in ewes. Br J Nutr 54, 713–718.CrossRefGoogle ScholarPubMed
Martens, H & Blume, I (1986) Effect of intraruminal sodium and potassium concentrations and of the transmural potential difference on magnesium absorption from the temporarily isolated rumen of sheep. Quart J Exp Pysiol 71, 409–415.CrossRefGoogle ScholarPubMed
Martens, H, Gabel, G & Strozyk, H (1987) The effect of potassium and the transmural potential difference on magnesium transport across an isolated preparation of sheep rumen epithelium. Q J Exp Physiol 72, 181–188.CrossRefGoogle ScholarPubMed
Martens, HS, Leonhard-Marek, S, Lang, I & Schweigel, M (1998) Sodium, potassium and magnesium in ruminants: interactions and antagonisms. Proceedings of the 10th International Conference of Production Diseases in Farm Animals, pp. 17–27 [Wensing, T, editor]. Wageningen: Wageningen Pers.Google Scholar
Newton, GL, Fontenot, JP, Tucker, RE & Polan, CE (1972) Effects of high dietary potassium intake on the metabolism of magnesium by sheep. J Anim Sci 35, 440–445.CrossRefGoogle Scholar
Perkin-Elmer, (1982) Analytical Method for Atomic Absorption Spectroscopy Handbook. Norwalk, CT: Perkin-Elmer.Google Scholar
Poe, JH, Greene, LW, Schelling, GT, Byers, FM & Ellis, WC (1985) Effects of dietary potassium and sodium on magnesium utilization in sheep. J Anim Sci 60, 578–582.Google Scholar
Quinlan, KP & DeSesa, MA (1955) Spectrophotometric Determination of Phosphorus as Molybdovanadophosphoric Acid. Anal Chem 27, 1626–1629.Google Scholar
Rahnema, SH & Fontenot, JP (1986) Effect of potassium on association of minerals with various fractions of digesta and feces of sheep fed hay. J Anim Sci 63, 1491–1501.CrossRefGoogle Scholar
Ram, L, Schonewille, JT, Martens, H, Van't Klooster, AT & Beynen, AC (1998) Magnesium absorption by wethers fed potassium bicarbonate in combination with different dietary magnesium concentrations. J Dairy Sci 81, 2485–2492.CrossRefGoogle ScholarPubMed
Rogers, PAM & Van't Klooster, AT (1969) The fate of Na, K, Ca, Mg and P in the digesta. In Mededelingen Landbouwhogeschool, pp. 26–39. Wageningen.Google Scholar
Schonewille, JT, Beynen, AC, Van't Klooster, AT, Wouterse, H & Ram, L (1999 a) Dietary potassium bicarbonate and potassium citrate have a greater inhibitory effect than does potassium chloride on magnesium absorption in wethers. J Nutr 129, 2043–2047.CrossRefGoogle ScholarPubMed
Schonewille, JT, Ram, L, Van't Klooster, AT, Wouterse, H & Beynen, AC (1997) Intrinsic potassium in grass silage and magnesium absorption in dry cows. Livest Prod Sci 48, 99–110.CrossRefGoogle Scholar
Schonewille, JT, Van't Klooster, AT, Wouterse, H & Beynen, AC (1999 b) Effects of intrinsic potassium in artificially dried grass and supplemental potassium bicarbonate on apparent magnesium absorption in dry cows. J Dairy Sci 82, 1824–1830.CrossRefGoogle ScholarPubMed
Scott, D (1966) The effects of sodium depletion and potassium supplements upon electrical potentials in the rumen of the sheep. Q J Exp Physiol 51, 60–69.Google Scholar
Tomas, FM & Potter, BJ (1976) The site of magnesium absorption from the ruminant stomach. Br J Nutr 36, 37–45.CrossRefGoogle ScholarPubMed
Van't Klooster, AT (1967) De toestand van calcium, magnesium en enkele andere mineralen in darminhoud en mest van herkauwers in verband met hun resorptie (The state of calcium, magnesium and some other minerals in gut contents and feces of ruminan relation to their absorption). PhD Thesis, Utrecht University, The Netherlands.Google Scholar
Wilkinson, L (1990) SYSTAT: The System for Statistics Everton, ILSYSTAT Incorporation.Google Scholar
Wylie, MJ, Fontenot, JP & Greene, LW (1985) Absorption of magnesium and other macrominerals in sheep infused with potassium in different parts of the digestive tract. J Anim Sci 61, 1219–1229.Google Scholar