Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-13T06:45:18.691Z Has data issue: false hasContentIssue false
  • English
  • Français

Response of putative indices of copper status to copper supplementation in human subjects

Published online by Cambridge University Press:  09 March 2007

Claire A. Kehoe ,
Eithne Turley ,
Maxine P. Bonham ,
Jacqueline M. O'Connor ,
Andrea McKeown ,
Marian S. Faughnan ,
James S. Coulter ,
William S. Gilmore ,
Alan N. Howard  and
J. J. Strain
Claire A. Kehoe
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Eithne Turley
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Maxine P. Bonham
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Jacqueline M. O'Connor
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Andrea McKeown
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Marian S. Faughnan
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
James S. Coulter
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
William S. Gilmore
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
Alan N. Howard
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
J. J. Strain*
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland
*
*Corresponding author: Professor J. J. Strain, fax +44 (0)1265 324965, email jj.strain@ulst.ac.uk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

No sensitive functional index is currently available to assess Cu status in healthy human populations. This study evaluated the effect of Cu supplementation on putative indices of Cu status in twelve women and twelve men, aged between 22 and 45 years, who participated in a double-blind placebo controlled crossover study. The study consisted of three 6-week supplementation regimens of 3 mg CuSO4, 3 mg Cu-glycine chelate and 6 mg Cu-glycine chelate, each separated by placebo periods of equal length. Women had significantly higher caeruloplasmin oxidase activity (P < 0·001), caeruloplasmin protein concentration (P < 0·05), and serum diamine oxidase activity (P < 0·01) at baseline than men. Erythrocyte and leucocyte superoxide dismutase activity, leucocyte cytochrome c oxidase activity, and erythrocyte glutathione peroxidase activity did not respond to Cu supplementation. Platelet cytochrome c oxidase activity was significantly higher (P < 0·01), after supplementation with 6 mg Cu-glycine chelate in the total group and in women but did not change in men. Caeruloplasmin oxidase activity was significantly higher (P < 0·05), in men after supplementation with 3 mg Cu-glycine chelate, while caeruloplasmin protein concentration was significantly lower in men after supplementation with 6 mg Cu-glycine chelate (P < 0·05). Serum diamine oxidase activity was significantly higher after all supplementation regimens in the total group and in both men and women (P < 0·01). These results indicate that serum diamine oxidase activity is sensitive to changes in dietary Cu intakes and may also have the potential to evaluate changes in Cu status in healthy adult human subjects.

Keywords

CopperDiamine oxidase
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 2 , August 2000 , pp. 151 - 156
Copyright
Copyright © The Nutrition Society 2000

References

Bayless, TM, Luk, GD, Baylin, SB and Thomas, ME (1981) Decreased plasma diamine oxidase levels in Crohn's disease and celiac disease. Gastroenterology 80, 1106.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Calvin, J and Price, CP (1986) Measurement of alpha 1 antichymotrypsin by immunoturbidity. Annals of Clinical Biochemistry 23, 296–299.CrossRefGoogle Scholar
Crawley, H (1992) Food Portion Sizes, 3rd ed. London: H.M. Stationery Office.Google Scholar
Danks, DM (1988) Copper deficiency in humans. Annual Review of Nutrition 8, 235–257.CrossRefGoogle ScholarPubMed
DiSilvestro, RA (1988) Influence of copper intake and inflammation on rat serum superoxide dismutase activity levels. Journal of Nutrition 118, 474–479.CrossRefGoogle ScholarPubMed
DiSilvestro, RA (1990) Influence of dietary copper, copper injections and inflammation on rat serum ceruloplasmin activity levels. Nutrition Research 10, 355–358.CrossRefGoogle Scholar
DiSilvestro, RA, Jones, AA, Smith, D and Wildman, R (1997) Plasma diamine oxidase activities in renal dialysis patients, a human with spontaneous copper deficiency and marginally copper deficient rats. Clinical Biochemistry 30, 559–563.CrossRefGoogle ScholarPubMed
Fisher, PWF, L'Abbé, MR and Giroux, A (1990) Effects of age, smoking, drinking, exercise and estrogen use on indices of copper status in healthy adults. Nutrition Research 10, 1081–1090.CrossRefGoogle Scholar
Gallagher, SK, Johnson, LK and Milne, DB (1989) Short-term and long-term variability of indices related to nutritional status. I: Ca, Cu. Fe, Mg, and Zn. Clinical Chemistry 35, 369–373.CrossRefGoogle ScholarPubMed
Henry, RJ, Chiamori, N, Jacobs, SL and Segalove, M (1960) Determination of caeruloplasmin oxidase in serum. Proceedings of the Society for Experimental Biology and Medicine 104, 620–624.CrossRefGoogle Scholar
Johnson, PE, Milne, DB and Lykken, GI (1992) Effects of age and sex on copper absorption, biological half-life, and status in humans. American Journal of Clinical Nutrition 56, 917–925.CrossRefGoogle ScholarPubMed
Johnson, WT, DuFault SN and Thomas, AC (1993) Platelet cytochrome. c oxidase activity is an indicator of copper status in rats. Nutrition Research 13, 1153–1162.CrossRefGoogle Scholar
Jones, A, DiSilvestro, RA, Coleman, M and Wagner, TL (1997) Copper supplementation of adult men: effects on blood copper enzyme activities and indicators of cardiovascular disease risk. Metabolism 46, 1380–1383.CrossRefGoogle ScholarPubMed
Jones, DG and Suttle, NF (1981) Some effects of copper deficiency on leucocyte function in cattle and sheep. Research in Veterinary Science 31, 151–156.CrossRefGoogle Scholar
Kehoe, CA, Faughnan, MS, Gilmore, WS, Coulter, JS, Howard, AN and Strain, JJ (2000) Plasma diamine oxidase activity is greater in copper adequate than copper marginal or copper deficient rats. Journal of Nutrition 130, 30–33.CrossRefGoogle ScholarPubMed
Klevay, LM, Buchet, JP, Bunker, VW, Clayton, BE, Gibson, RS, Medeiros, DM, Moser, Veillon PBL, Payterson, KY, Taper, LJ, & Wolf, WR (1993) Copper in the western diet (Belgium, Canada, UK and USA). In Trace elements in Man and Animals-TEMA 8 pp. 207–210 ]Anke, M, Meisser, D and Mills, CF, editors]. Gersdorf, Germany: Verlag Media Touristik.Google Scholar
Kusche, J, Trotha, UV, Muhlberger, G and Lorenz, W (1974) The clinical-chemical application of the NADH test for the determination of diamine oxidase activity in human pregnancy. Agents in Action 4/3, 188–189.CrossRefGoogle Scholar
Livingstone, BME, Prentice, AM, Coward, WA, Strain, JJ, Black, AK, Davies, PSW, Stewart, CM, McKenna, PG and Whitehead, RG (1992) Validation of estimates of energy intake by weighed dietary record and diet history in children and adolescents. American Journal of Clinical Nutrition 56, 29–35.CrossRefGoogle ScholarPubMed
Medeiros, DM, Milton, A, Brunett, E and Stacy, L (1991) Copper supplementation effects on indicators of copper status and serum cholesterol in adult males. Biological Trace Element Research 30, 19–35.CrossRefGoogle ScholarPubMed
Milne, DB (1994) Assessment of copper nutritional status. Clinical Chemistry 40, 1479–1484.CrossRefGoogle ScholarPubMed
Milne, DB and Forrest, FH (1996) Effects of a diet low in copper on copper-status indicators in postmenopausal women. American Journal of Clinical Nutrition 63, 358–364.CrossRefGoogle ScholarPubMed
Milne, DB and Johnson, PK (1993) Assessment of copper status: effect of age and gender on ranges in healthy adults. Clinical Chemistry 39, 883–887.CrossRefGoogle ScholarPubMed
Nielsen, FH, Milne, DB, Mullen, LM and Gallagher, SK (1990) Dietary sulfur amino-acids, and genetic make-up or interindividual variation affect the response to copper depletion. Journal of Trace Element and Experimental Biology 3, 281–296.Google Scholar
Olivares, M and Uauy, R (1996) Copper as an essential nutrient. American Journal of Clinical Nutrition 63, 791S–796S.CrossRefGoogle ScholarPubMed
Paglia, DE and Valentine, WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory Clinical Medicine 70, 158–169.Google ScholarPubMed
Pratt, WB, Omdahl, JL and Sorenson, JRJ (1985) Lack of effects of copper gluconate supplementation. American Journal of Clinical Nutrition 42, 681–682.CrossRefGoogle ScholarPubMed
Prohaska, JR (1997) Response of rat cuproenzymes to variable dietary copper. Journal of Nutritional Biochemistry 8, 316–321.CrossRefGoogle Scholar
Salmenpera, L, Siimes, MA, Nanto, V and Perheentupa, J (1989) Copper supplementation: failure to increase plasma copper and ceruloplasmin concentrations in healthy infants. American Journal of Clinical Nutrition 50, 843–847.CrossRefGoogle ScholarPubMed
Senn, S (1993) The AB/BA design with normal data. In Cross-over Trials in Clinical Research, pp. 31–60 ]Barnett, Y, editor]. Chichester: J. Wiley & Sons.Google Scholar
Smith, L (1955) Spectrophotometric assay of cytochrome c oxidase. In Methods of Biochemical Analysis pp. 427–434. New York, NY: J. Wiley & Sons.CrossRefGoogle Scholar
Solomons, NW (1979) On the assessment of zinc and copper nutriture in man. American Journal of Clinical Nutrition 32, 856–871.CrossRefGoogle ScholarPubMed
Takagi, K, Nakao, M, Ogura, Y, Nabeshima, T and Kunii, A (1994) Sensitive colorimetric assay of serum diamine oxidase. Clinical Chimica Acta 226, 67–75.CrossRefGoogle ScholarPubMed
Tam, CF, Kopple, JD, Wang, M and Swendseid, ME (1979) Diamine oxidase activity in plasma and urine in uremia. Nephron 23, 23–27.Google ScholarPubMed
Turnlund, JR (1994) Copper. In Modern Nutrition in Health and Disease 8th ed., pp. 231–241 ]Shils, ME, Olson, JA and Shike, M, editors]. Philadelphia, PA: Lea & Febiger.Google Scholar
Turnlund, JR, Keen, CL and Smith, RJ (1990) Copper status and urinary and salivary copper in young men at three levels of dietary copper. American Journal of Clinical Nutrition 51, 658–664.CrossRefGoogle ScholarPubMed
Turnlund, JR, Scott, KC, Peiffer, GL, Jang, AM, Keyes, WR, Keen, CL and Sakanashi, TM (1997) Copper status of young men consuming a low-copper diet. American Journal of Clinical Nutrition 65, 72–78.CrossRefGoogle ScholarPubMed
Wolvekamp, MC and deBruin RW (1994) Diamine oxidase: an overview of historical, biochemical and functional aspects. Digestive Diseases 12, 2–14.CrossRefGoogle ScholarPubMed