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Rumen simulation technique study on the interactions of dietary lauric and myristic acid supplementation in suppressing ruminal methanogenesis

Published online by Cambridge University Press:  09 March 2007

Carla R. Soliva ,
Leo Meile ,
Adam Cieślak ,
Michael Kreuzer  and
Andrea Machmüller
Carla R. Soliva*
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Leo Meile
Affiliation:
Institute of Food Science and Nutrition, Laboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Adam Cieślak
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland Department of Animal Nutrition and Feed Management, Agricultural University of Poznań, ul. Wolynska 33, 60-637 Poznań, Poland
Michael Kreuzer
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Andrea Machmüller
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
*
*Corresponding author: Dr Carla R. Soliva, fax +41 1 632 1128, email carla.soliva@inw.agrl.ethz.ch
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Abstract

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The interactions of lauric (C12) and myristic acid (C14) in suppressing ruminal methanogenesis and methanogens were investigated with the rumen simulation technique (Rusitec) using bovine ruminal fluid. The fatty acids were added to basal substrates (grass hay:concentrate, 1:1.5) at a level of 48 g/kg DM, provided in C12:C14 ratios of 5:0, 4:1, 3:2, 2·5:2.5, 2:3, 1:4 and 0:5. Additionally, an unsupplemented control consisting of the basal substrates only was employed. Incubation periods lasted for 15 (n 4) and 25 (n 2) d. CH4 formation was depressed by any fatty acid mixture containing at least 40 % C12, and effects persisted over the complete incubation periods. The greatest depression (70 % relative to control) occurred with a C12:C14 ratio of 4:1, whereas the second most effective treatment in suppressing CH4 production (60 % relative to control) was found with a ratio of 3:2. Total methanogenic counts were decreased by those mixtures of C12 and C14 also successful in suppressing methanogenesis, the 4:1 treatment being most efficient (60 % decline). With this treatment in particular, the composition of the methanogenic population was altered in such a way that the proportion of Methanococcales increased and Methanobacteriales decreased. Initially, CH4 suppression was associated with a decreased fibre degradation, which, however, was reversed after 10 d of incubation. The present study demonstrated a clear synergistic effect of mixtures of C12 and C14 in suppressing methanogenesis, mediated probably by direct inhibitory effects of the fatty acids on the methanogens.

Keywords

MethaneMedium-chain fatty acidsLipidsRusitec
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 4 , October 2004 , pp. 689 - 700
Copyright
Copyright © The Nutrition Society 2004

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