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Studies on the bioavailability of the provitamin A carotenoid, β-carotene, using human exfoliated colonic epithelial cells

Published online by Cambridge University Press:  09 March 2007

T. Gireesh ,
P. P. Nair  and
P. R. Sudhakaran
T. Gireesh
Affiliation:
Department of Biochemistry, University of Kerala, Trivandrum, India695 581
P. P. Nair
Affiliation:
Department of International Health, Johns Hopkins University, Baltimore, USA
P. R. Sudhakaran*
Affiliation:
Department of Biochemistry, University of Kerala, Trivandrum, India695 581
*
*Corresponding author: fax +91 471 2307158, email prsbn@md4.vsnl.net.in
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Abstract

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The possibility of using exfoliated colonic epithelial cells for assessing the bioavailability of β-carotene was examined. Analysis of exfoliated colonic epithelial cells showed the presence of β-carotene and vitamin A. The β-carotene content was significantly lower in cells from stool samples of subjects on a β-carotene-poor diet than those receiving a single dose of a β-carotene supplement. Colonic epithelial cells isolated from stool samples collected daily during a wash-out period while the subjects were on a β-carotene-poor diet showed a steady decrease in β-carotene content, reaching the lowest value on day 7. Kinetic analysis showed that a single dose of a β-carotene supplement in the form of spirulina (Spirulina platensis) or agathi (Sesbania grandiflora) after the wash-out period caused an increase in the β-carotene content after a lag period of 5–7 d, but the vitamin A levels during these periods were not significantly affected. Analysis of plasma β-carotene concentration also showed similar changes, which correlated with those of exfoliated colonic cells. A relationship between the β-carotene content of the diet and that of the colonic epithelial cells suggests that analysis of the β-carotene content in exfoliated human colonic epithelial cells is a useful non-invasive method to assess the bioavailability of provitamin A β-carotene.

Keywords

BioavailabilityVitamin Aβ-CaroteneColonic epithelial cells
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 2 , August 2004 , pp. 241 - 245
Copyright
Copyright © The Nutrition Society 2004

References

Albaugh, GP, Iyengar, V, Lohani, A, Malayeri, M,Bala, S&Nair, PPIsolation of exfoliated colonic epithelial cells, a novel, non-invasive approach to the study of cellular markers Int J Cancer (1992) 52 347–350Google Scholar
Bennett, CA&Franklin, NLMathematical machinery Statistical Analysis in Chemistry and the Chemical Industry New YorkJohn Wiley & Sons, Inc (1967) 677–684Google Scholar
Edwards, AJ, You, CS, Swanson, JE&Parker, RSA novel extrinsic reference method for assessing the vitamin A value of plant foods Am J Clin Nutr (2001) 74 348–355CrossRefGoogle ScholarPubMed
Frank, HAStructural determinations and spectroscopic studies of carotenoids reveal the factors important in controlling their function in biological systems Eur J Clin Nutr (1996) 50 S13–S16Google Scholar
Gireesh, T, Jayadeep, A, Rajasekharan, KN,Menon, VP, Vairamany, M,Tang, G, Nair, PP&Sudhakaran, PRProduction of deuterated β-carotene by metabolic labeling of Spirulina platensis Biotechnol Lett (2001) 23 447–449Google Scholar
Gopalan, C, Ramasasthri, BV&Balasubramanyan, SCNutritive Value of Indian FoodIndian Council of Medical Research HyderabadNational Institute of Nutrition (1995) 43–97Google Scholar
Lepkin, MProliferation and differentiation of gastrointestinal cells Phys Rev (1973) 53 891–915Google Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL&Randall, RJProtein measurement with the Folin–phenol reagent J Biol Chem (1951) 193 265–275Google Scholar
Nair, PP, Lohani, A, Norkus, EP, Feagins, H&Bhagavan, HNUptake and distribution of carotenoids, retinol and tocopherols in human colonic epithelial cells in vivo Cancer Epidemiol Biomarker Prev (1996) 5 913–916Google ScholarPubMed
Ribaya-Mercado, JD, Solon, FS, Solon, MA,Cabel-Barza, MA, Perfecto, CS,Tang, G, Solon, JA, Fjeld, CR &Russel, RMBioconversion of plant carotenoids to vitamin A in Filipino school-aged children varies inversely with vitamin A status Am J Clin Nutr (2000) 72 455–465Google Scholar
Van den Berg, HVitamin A intake and status Eur J Clin Nutr (1996) 50 S7–S12Google ScholarPubMed
Van het Hof, K, West, CE, Weststrate, JA&Hautvast, JGAJDietary factors that affect the bioavailability of caroteniods J Nutr (2000) 130 503–506CrossRefGoogle Scholar
Ye, X, Al-Babili, S, Kloti, A, Zhang, J, Lucca, P,Beyer, P&Potrykus, I, Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm Science (2000) 287 303–305CrossRefGoogle ScholarPubMed