Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-28T03:01:36.940Z Has data issue: false hasContentIssue false
  • English
  • Français

Tissue-specific modification of selenium concentration by acute and chronic dexamethasone administration in mice

Published online by Cambridge University Press:  09 March 2007

Chiho Watanabe ,
Choong-Yong Kim  and
Hiroshi Satoh
Chiho Watanabe
Affiliation:
Department of Environmental Health Sciences, Tohoku University School of Medicine, Sendai, 980-77Japan
Choong-Yong Kim
Affiliation:
Department of Environmental Health Sciences, Tohoku University School of Medicine, Sendai, 980-77Japan
Hiroshi Satoh
Affiliation:
Department of Environmental Health Sciences, Tohoku University School of Medicine, Sendai, 980-77Japan
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Several clinical reports have shown changes in plasma Se concentration with corticosteroid treatments, but the results have been inconsistent. Few experimental studies have been done on this subject. In the present study the effect of dexamethasone (DEX) treatment on Se concentrations and activities of Se-dependent glutathione peroxidase (EC 1.11.1.9; SeGPx) were examined in adult male ICR mice. In the first experiment, DEX was given via drinking water containing 5 or 50 mg DEX/I. At 1 or 3 weeks of DEX treatment, mice were dissected and the Se concentrations as well as SeGPx activities in various tissues, including plasma, were determined. At 1 week the DEX-treated groups had significantly lower hepatic Se concentrations and significantly higher plasma and cerebral concentrations than the control group. The DEX-treated groups showed lower SeGPx activities in the hepatic cytosol and higher SeGPx activities in the plasma than the saline (9 g NaCl/l)-treated group, in parallel with the changes in Se concentrations. At 3 weeks, neither hepatic nor plasma Se concentrations showed a significant change. In the second experiment, mice were injected subcutaneously with DEX and, thereafter, mice were food-deprived. The DEX-injected groups had higher plasma Se concentrations. A similar finding was obtained also when the DEX- or saline-injected mice were not food-deprived. Thus, the difference between the DEX-treated and control groups was possibly caused by redistribution of tissue Se. These results suggested that the effects of DEX on Se concentrations were tissue dependent and that the higher plasma Se observed in DEX-treated groups might be explained by the release of tissue Se into plasma as plasma SeGPx.

Keywords

SeleniumGlutathione peroxidaseDexamethasone
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 3 , September 1997 , pp. 501 - 508
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Avissar, N., Whitin, J. C., Allen, P. Z., Palmer, I. S. & Cohen, H. J. (1989) Antihuman plasma glutathione peroxidase antibodies: immunologic investigations to determine plasma glutathione peroxidase protein and selenium content in plasma. Blood 73, 318–323.CrossRefGoogle ScholarPubMed
Bansal, M., Oborn, C., Danielson, K. & Medina, D. (1989) Evidence for two selenium-binding proteins distinct from glutathione peroxidase in mouse liver. Carcinogenesis 10, 541–546.CrossRefGoogle ScholarPubMed
Behne, D., Hilmert, H., Scheid, S., Gessner, H. & Elger, W. (1988) Evidence for specific selenium target tissues and new biologically important selenoproteins. Biochimica et Biophysica Acta 966, 12–21.CrossRefGoogle ScholarPubMed
Behne, D. & Wolters, W. (1983) Distribution of selenium and glutathione peroxidase in the rat. Journal of Nutrition 113, 456–461.CrossRefGoogle ScholarPubMed
Bryson, G. & Reynolds, R. W. (1981) Effects of adrenocortical stress responses and metabolic functions during and after long-term administration of dexamethasone to adult Evans–Long rats. Research Communications in Chemical Pathology and Pharmacology 34, 267–286.Google ScholarPubMed
Burk, R. (1993) Regulation of selenoproteins. Annual Review of Nutrition 13, 65–81.CrossRefGoogle ScholarPubMed
Chu, F. F., Esworthy, R. S., Doroshow, J. H., Doan, K. & Liu, X. F. (1992) Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents. Blood 79, 3233–3238.CrossRefGoogle ScholarPubMed
Cousins, R. J., Dunn, M. A., Leinart, A. S., Yedinak, K. C. & DiSilvestro, R. A. (1986) Coordinate regulation of zinc metabolism and metallothionein gene expression in rats. American Journal of Physiology 251, E688–E694.Google ScholarPubMed
Deagen, J., Butler, J., Zachara, B. & Whanger, P. (1993) Determination of the distribution of selenium between glutathione peroxidase, selenoprotein P, and albumin in plasma. Analytical Biochemistry 208, 176–181.CrossRefGoogle ScholarPubMed
Dunnet, C. (1955) A multiple comparison procedure for comparing several treatments with a control. American Statistical Association Journal 50, 1096–1121.CrossRefGoogle Scholar
Finley, J. & Kincaid, P. (1991) Effect of sex and time of sampling on selenium and glutathione peroxidase activity in tissues of mature rats. Biological Trace Element Research 29, 181–191.CrossRefGoogle ScholarPubMed
Hawker, F., Stewart, P. & Snitch, P. (1990) Effects of acute illness on selenium homeostatis. Critical Care Medicine 18, 442–446.CrossRefGoogle Scholar
Koskelo, E. K. (1990) Serum selenium in children during anti-cancer chemotherapy. European Journal of Clinical Nutrition 44, 799–802.Google ScholarPubMed
LaBorde, J. B., Hansen, D. K., Young, J. F., Sheehan, D. M. & Holson, R. R. (1992) Prenatal dexamethasone exposure in rats: effects of dose, age at exposure, and drug-induced hypophagia on malformations and fetal organ weights. Fundamental and Applied Toxicology 19, 545–554.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Marano, G., Fischioni, P., Graziano, C., Iannone, M. & Morisi, G. (1990) Increased serum selenium levels in patients under corticosteroid treatment. Pharmacology and Toxicology 67, 120–122.CrossRefGoogle ScholarPubMed
Olin, K., Walter, R. & Keen, C. (1994) Copper deficiency affects selenoglutathione peroxidase and selenodeiodinase activities and antioxidant defense in weanling rats. American Journal of Clinical Nutrition 59, 654–658.CrossRefGoogle ScholarPubMed
Paglia, D. E. & Valnetine, W. N. (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase in developing rat brain. Journal of Laboratory and Clinical Medicine 70, 158–169.Google Scholar