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Utilization of 14C-labelled Bacillus subtilis and Escherichia coli by sheep

Published online by Cambridge University Press:  09 March 2007

N. J. Hoogenraad ,
F. J. R. Hird ,
R. G. White  and
R. A. Leng
N. J. Hoogenraad
Affiliation:
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia
F. J. R. Hird
Affiliation:
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia
R. G. White
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW, Australia
R. A. Leng
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW, Australia
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Abstract

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1. Bacillus subtilis and Escherichia coli were grown on 14C-labelled glucose and used for the preparation of labelled whole cells, cell walls, cell contents and peptidoglycan.

2. The radioactive samples were injected into the abomasum of sheep and the 14C appearing in expired air, plasma glucose, urine and faeces was determined. Whole cells were also injected into the rumen and the incorporation of 14C into volatile fatty acids was measured.

3. All the bacterial preparations, including cell walls, were extensively digested and absorbed, Less than 15% of the radioactivity was recovered in the faeces.

4. Up to 20% of the radioactivity injected was recovered in expired carbon dioxide with only 2.4–8.1% passing through the glucose pool.

5. It has been calculated that under the conditions of the experiment 18.5 % of the total glucose entering the body pool of glucose in 24 h was derived from bacterial carbon.

Type
Research Article
Information
British Journal of Nutrition , Volume 24 , Issue 1 , March 1970 , pp. 129 - 144
Copyright
Copyright © The Nutrition Society 1970

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