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Utilization of α-amylase (EC 3.2.1.1) resistant maize and pea (Pisum sativum) starch in the rat

Published online by Cambridge University Press:  09 March 2007

R. M. Faulks ,
Susan Southon  and
G. Livesey
R. M. Faulks
Affiliation:
Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
Susan Southon
Affiliation:
Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
G. Livesey
Affiliation:
Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
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Abstract

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1. The extent of utilization of α-amylase (EC 3.2.1.1)-resistant retrograded starches in vivo was assessed in male Wistar rats (about 100 g body-weight). All animals were given a fibre-free semi-synthetic basal diet (SS) containing sucrose as the only carbohydrate source, ad lib., for 13 d. On day 14, after an overnight fast, rats were allocated to one of five dietary treatments (n 30):1, fibre-free basal SS diet; 2-5, basal SS diet supplemented with 100 g sucrose, Solka floc (cellulose), resistant maize starch (RCS) or resistant pea (Pisum sativum) starch (RPS)/kg diet. Animals allocated to each dietary treatment were divided into three groups of ten rats which were given the appropriate diet for 8 or 9, 17 or 18 and 29 or 30 d (8/9, 17/18 or 29/30 d groups respectively). Rats were fed on 12 g diet/d (treatment 1) or 13.2 g diet/d (treatments 2-5) for the first 20 d, and 14 or 15.4 g/d respectively until the end of the experiment. Rats fed on the supplemented basal diets were thus given 10% more food to allow for the addition of the test carbohydrate. Faecal carbohydrate excretion was determined at intervals in the 17/18 d groups. At the end of each experimental period animals were killed after consuming their daily food ration and small intestinal length, weight of caecal and ileal contents and tissue, and pH of caecal contents measured. The amount of carbohydrate in the caecal and ileal contents from the 8/9 and 17/18 d groups was determined.

2. Weights of ileal and caecal contents, caecal tissue and faecal output were significantly greater at all time points for rats fed on the resistant starches compared with those fed on basal and sucrose-supplemented diets. Values were higher for RPS-fed rats than for RCS-fed rats.

3. The quantity of carbohydrate recovered from ileal and caecal contents showed that both RCS and RPS were partially digested and absorbed as carbohydrate, but that RPS was digested to a lesser extent.

4. The concentration of carbohydrate decreased between the ileal and caecal sites when RPS and RCS were given but was essentially unchanged when cellulose was given. This is consistent with rapid fermentation of a fraction of these starches.

5. Faecal carbohydrate elimination in the 17/18 d groups fed on RCS and RPS declined with time, which suggested an adaptive response resulting in increased utilization of the starches. This adaptive response was slower in the RPS-fed rats than the RCS-fed rats.

6. The present study indicates that, in the rat, there are significant differences in the utilization of resistant starches from different sources.

Type
Research Article
Information
British Journal of Nutrition , Volume 61 , Issue 2 , March 1989 , pp. 291 - 300
Copyright
Copyright © The Nutrition Society 1989

References

Berry, C. S. (1986) Resistant starch: formation and measurement of starch that survives exhaustive digestion with amylolytic enzymes during the determination of dietary fibre. Journal of Cereal Science 4, 301–314.CrossRefGoogle Scholar
Bjorck, I., Nyman, M., Pedersen, B., Siljestrom, M., Asp., N-G. & Eggum, B. O. (1986) On the digestibility of starch in wheat bread - studies in vitro and in vivo. Journal of Cereal Science 4, 1–11.CrossRefGoogle Scholar
Collinson, R. (1968). In Starch and its Derivatives, pp. 194–201 [J. A. Radley, editor]. London: Chapman & Hall.Google Scholar
Dreher, M. L., Dreher, C. J. & Berry, J. W. (1984) Starch digestibility of foods: a nutritional perspective. CRC Critical Reviews in Food Science and Nutrition 20, 47–71.CrossRefGoogle Scholar
Englyst, H. N. & Cummings, J. H. (1985) Digestion of the polysaccharides of some cereal foods in the human small intestine. American Journal of Clinical Nutrition 42, 778–787.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Macfarlane, G. T. (1986) Breakdown of resistant and readily digestible starch by human gut bacteria. Journal of the Science of Food and Agriculture 37, 699–706.CrossRefGoogle Scholar
Englyst, H. N., Wiggins, H. S. & Cummings, J. H. (1982) Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of the constituent sugars as alditol acetates. Analyst 107, 307–318.CrossRefGoogle ScholarPubMed
Foster, J. F. (1965). In Starch Chemistry and Technology, Vol. 1, pp. 350–353. [Whistler, R.L. and Paschall, E. F., editors]. London Academic Press.Google Scholar
Holm, J., Bjorck, I., Ostrowska, S., Eliasson, A.-C., Asp, N.-G., Larsson, K. & Lundquist, I. (1983) Digestibility of amylose-lipid complexes in vitro and in vivo. Starch/Starke 35, 294–297.CrossRefGoogle Scholar
Johnson, I. T. & Gee, J. M. (1986) Gastrointestinal adaptation in response to soluble non-availabile polysaccharides in the rat. British Journal of Nutrition 55, 497–505.CrossRefGoogle ScholarPubMed
Johnson, I. T., Gee, J. M. & Mahoney, R. R. (1984) Effects of dietary supplements of guar gum and cellulose on intestinal cell proliferation, enzyme levels and sugar transport in the rat. British Journal of Nutrition 52, 477–487.CrossRefGoogle ScholarPubMed
Kerr, R. W. (1950) Chemistry and Industry of Starch, pp. 166–167. New York: Academic Press.Google Scholar
McLean, R. A. H., Eastwood, M. A., Brydon, W. G., Anderson, J. R. & Anderson, D. M. W. (1983) A study of the effects of dietary gum arabic in humans. American Journal of Clinical Nutrition 37, 368–375.Google Scholar
Nyman, M. & Asp, N.-G. (1982) Fermentation of dietary fibre components in the rat intestinal tract. British Journal of Nutrition 47, 357–366.CrossRefGoogle ScholarPubMed
Nyman, M., Asp, N.-G., Cummings, J. H. & Wiggins, H. (1986) Fermentation of dietary fibre in the intestinal tract: comparison between man and rat. British Journal of Nutrition 55, 487–496.CrossRefGoogle Scholar
Saeman, J. F., Moore, W. E., Mitchell, R. L. & Millet, M. A. (1954) Techniques for the determination of pulp constituents by quantitative paper chromatography. TAPPI 37, 336–343.Google Scholar
Swinkels, J. J. M. (1985) Composition and properties of commercial native starches. Starch/Starke 37, 1–5.CrossRefGoogle Scholar
Wyatt, G. & Horn, N. (1988) Fermentation of resistant food starches by human and rat intestinal bacteria. Journal of the Science of Food and Agriculture 44, 281–288.CrossRefGoogle Scholar