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Variation between sheep in renal excretion of [14C]allantoin

Published online by Cambridge University Press:  09 March 2007

P. Prasitkusol ,
E. R. Ørskov ,
X. B. Chen ,
F. D. DeB. Hovell  and
D. J. Kyle
P. Prasitkusol
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
E. R. Ørskov*
Affiliation:
International Feed Resources Unit, Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH, Scotland, UK
X. B. Chen
Affiliation:
Lucta (Guangzhou) Flavours Co. Ltd., 173-175 Youyi Road, Guangzhou Economic & Technological Development District, Guangzhou 510730, People's Republic of China
F. D. DeB. Hovell
Affiliation:
Department of Agriculture and Forestry, University of Aberdeen, 581 King Street, Aberdeen AB24 5UA, Scotland, UK
D. J. Kyle
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
*
†Corresponding author: Professor E. R. Ørskov, fax +44 1224 311 556, email b.orskov@mluri.sari.ac.uk
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Abstract

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The objectives of the present study were to investigate the recovery of [14C]allantoin in urine of sheep dosed intravenously and degradation of allantoin by rumen micro-organisms. The recovery of [14C]allantoin in the urine of eight sheep was measured during three periods in two experiments. Individual values of [14C]allantoin recovery varied from 66 to 95 % (mean value 83 (SE 1·6) %). The recovery of [14C]allantoin showed no relation to the level of feed intake. There was some evidence that glomerular filtration rate was an important factor affecting the amount of urinary allantoin recovered in one experiment. Incomplete recovery of plasma [14C]allantoin in the urine indicated losses of plasma [14C]allantoin via non-renal routes. This is supported by the disappearance of 14C from rumen contents incubated in vitro with [14C]allantoin for 48 h (88 %) and the presence of 14C in saliva in vivo from sheep sampled after dosing with [14C]allantoin. However, the amount of 14C activity in the saliva was very low (equivalent to only 1·5 % of the total dose in sheep producing saliva at a rate of 15 litres/d). The proportion of renal and non-renal excretion of purine derivatives was found to be unpredictable both between and within individual animals. The factors responsible for this variability need to be identified, and existing models of excretion of purine derivatives may need to be modified accordingly to improve their accuracy of prediction. A single intravenous injection of [4,5-14C]allantoin provides a simple alternative to infusion methods used to measure the proportion of plasma allantoin excreted in the urine of sheep. Using this method it may be feasible to validate PD excretion models in other ruminant livestock.

Keywords

Renal excretionPurine derivativesAllantoinRadioactivitySheep
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 6 , June 2002 , pp. 561 - 568
Copyright
Copyright © The Nutrition Society 2002

References

Association of Official Analytical Chemists (1980) Official Methods of Analysis, 13th ed. Washington, DC: AOAC.Google Scholar
Ayala, A & Hovell, FDDeB (1997) Nitrogen balance and rumen microbial nitrogen supply measured in Zebu bulls given young or mature Napier grass (Pennisetum purpureum). Proceedings of the Nutrition Society 56, 186AGoogle Scholar
Balcells, J, Guada, JA, Castrillo, C & Gasa, J (1991) Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116, 309–317.CrossRefGoogle Scholar
Chen, XB, Fujihara, T, Nakamura, K, Mawuenyegah, PO, Franklin, MF & Kyle, DJ (1997) Response of urinary and plasma purine derivatives to various rates and infusion patterns of purines in sheep nourished by intragastric infusion. Journal of Agricultural Science, Cambridge 129, 343–352.CrossRefGoogle Scholar
Chen, XB, Hovell, FDDeB & Ørskov, ER (1990 a) Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut. British Journal of Nutrition 63, 197–205.CrossRefGoogle ScholarPubMed
Chen, XB, Hovell, FDDeB, Ørskov ER & Brown, DS (1990 b) Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.CrossRefGoogle ScholarPubMed
Chen, XB, Kyle, DJ & Ørskov ER (1993) Measurement of allantoin in urine and plasma by high-performance liquid chromatography with pre-column derivatization. Journal of Chromatography 617, 241–247.CrossRefGoogle ScholarPubMed
Chen, XB, Kyle, DJ, Ørskov, ER & Hovell, FDDeB (1991) Renal clearance of plasma allantoin in sheep. Experimental Physiology 76, 59–65.CrossRefGoogle ScholarPubMed
Chen, XB, Mathieson, J & Hovell, FDDeB (1990 c) Measurement of purine derivatives in urine of ruminants using automated methods. Journal of the Science of Food and Agriculture 53, 23–33.CrossRefGoogle Scholar
Chen, XB, Susmel, P, Stefanon, B & Ørskov, ER (1995) On the use of purine derivatives in spot urine, plasma and milk samples as indicators of microbial protein supply in sheep and cattle. In Protein Metabolism and Nutrition. Proceedings of the 7th International Symposium on Protein Metabolism and Nutrition; Vale de Santarem – Portugal, vol. 81, pp. 325–329. Portugal: Estação Zootécnia, Instituto Nacional de Investigação Agrária.Google Scholar
Coleman, GS (1968) The metabolism of bacterial nucleic acid and of free components of nucleic acid by the rumen ciliate Entodinium caudotum. Journal of General Microbiology 54, 83–96.CrossRefGoogle Scholar
Emslie-Smith, D, Paterson, CR, Scratcherd, T & Read, WN (1988) The Kidney. Textbook of Physiology, 11th ed. Edinburgh: Churchill Livingstone.Google Scholar
Fujihara, T, Ørskov, ER, Reeds, PJ & Kyle, DJ (1987) The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.CrossRefGoogle Scholar
Hawk, PB, Oser, BL & Summerson, D (1965) Determination of creatinine. In Practical Physiological Chemistry, vol. 12, pp. 506–509: The Blakiston Co.Google Scholar
Kahn, LP & Nolan, JV (2000) Kinetic of allantoin metabolism in sheep. British Journal of Nutrition 84, 629–634.CrossRefGoogle ScholarPubMed
Kay, RNB (1960) The rate of flow and composition of various salivary secretions in sheep and calves. Journal of Physiology 150, 515–537.CrossRefGoogle ScholarPubMed
Liang, JB, Pimpa, O, Abdullah, N & Jelan, ZA (1999) Estimation of rumen microbial protein production from urinary purine derivatives in Zebu cattle and water buffalo. In Nuclear Based Technologies for Estimating Microbial Protein Supply in Ruminant Livestock, IAEA-TECDOC-1093, pp. 35–42. Vienna: IAEA.Google Scholar
Menke, KH & Steingass, H (1988) Estimation of the energenic feed value obtained from chemical analysis and in vitro gas production. Animal Research Development 28, 7–55.Google Scholar
Ørskov, ER, Grubb, DA, Wenham, G & Corrigall, W (1979) The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acid and protein. British Journal of Nutrition 41, 553–558.Google Scholar
Pimpa, O, Liang, JB, Jelan, ZA & Abdullah, N (2001) Urinary excretion of duodenal purine derivatives in Kedah-Kelantan cattle. Animal Feed Science and Technology 92, 203–214.Google Scholar
Prasitkusol, P (2001) Renal excretion and metabolism of uric acid and allantoin in sheep and cattle. PhD Thesis, University of Aberdeen.Google Scholar
Schäli, C & Roch-Ramel, F (1980) Renal handling of [14C]allantoin in the rabbit. Journal of Pharmacology and Experimental Therapeutics 213, 168–172.Google ScholarPubMed
Stefano, AD, Pizzichini, M, Leoncini, R, Vagnoni, DB, Pagani, R & Marinello, E (1992) Quantitative separation of uric acid and allantoin from rat liver tissue. Biochimica et Biophysica Acta 1117, 1–6.CrossRefGoogle ScholarPubMed
Surra, JC, Guada, JA, Balcells, J & Castrillo, C (1997) Renal and salivary clearance of purine derivatives in sheep. Journal of Animal Science 65, 83–91.CrossRefGoogle Scholar
Terzuoli, L, Pandolfi, ML, Arezzini, L, Pizzichini, M, Marinello, E & Pagani, R (1995) Separation and determination of liver uric acid and allantoin. Journal of Chromatography B 663, 143–147.CrossRefGoogle Scholar
Vogels, GD & Van Drift, C (1976) Degradation of purines and pyrimidines by microorganisms. Bacteriological Reviews 40, 403–468.CrossRefGoogle ScholarPubMed
Verbic, J, Chen, XB, MacLeod, NA & Ørskov, ER (1990) Excretion of purine derivatives by ruminants. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge 114, 243–248.Google Scholar