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Changes in the genetic composition of Myzus persicae nicotianae populations in Chile and frequency of insecticide resistance mutations

Published online by Cambridge University Press:  07 October 2021

Marco A. Cabrera-Brandt Open the ORCID record for Marco A. Cabrera-Brandt [Opens in a new window] ,
Amalia Kati ,
María E. Rubio-Meléndez ,
Christian C. Figueroa  and
Eduardo Fuentes-Contreras
Marco A. Cabrera-Brandt*
Affiliation:
Facultad de Ciencias Agrarias, Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Casilla 747, Talca, Chile
Amalia Kati
Affiliation:
Plant Pathology Laboratory, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece
María E. Rubio-Meléndez
Affiliation:
Facultad de Ingeniería, Centre for Bioinformatics and Molecular Simulation, Universidad de Talca, Casilla 747, Talca, Chile
Christian C. Figueroa
Affiliation:
Centre for Molecular and Functional Ecology in Agroecosystems, Instituto de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile
Eduardo Fuentes-Contreras
Affiliation:
Facultad de Ciencias Agrarias, Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Casilla 747, Talca, Chile
*
Author for correspondence: Marco A. Cabrera-Brandt, Email: mcabrerabrandt@gmail.com
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Abstract

Myzus persicae is a cosmopolitan aphid that is highly polyphagous and an important agricultural pest. The subspecies M. persicae nicotianae has been described for highly specialized phenotypes adapted to tobacco (Nicotiana tabacum). In Chile, the population of M. persicae nicotianae was originally composed of a single red genotype that did not possess insecticide resistance mutations. However, in the last decade, variation in the colour of tobacco aphids has been observed in the field. To determine whether this variation stems from the presence of new genotypes, sampling was carried out across the entire distribution of tobacco cultivation regions in Chile. The aphids collected were genotyped, and the frequency of kdr (L1014F), super-kdr (M918T), modification of acetylcholinesterase (MACE) and nicotinic acetylcholine receptor β subunit (nAChRβ) mutations associated with insecticide resistance was determined. A total of 16 new genotypes of M. persicae nicotianae were detected in Chile: four of them possessed the MACE mutation, and none of them possessed the kdr, super-kdr or nAChRβ mutation. The previously described red genotype was not detected in any of the sampled fields over two seasons. These results raise questions about the mechanisms underlying changes in the genetic structure of M. persicae nicotianae populations in Chile. Future research aimed at addressing these questions could provide new insight into aphid evolution and agricultural practices.

Keywords

Clonal diversitymutational insecticide resistanceMyzus persicae nicotianae
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 6 , December 2021 , pp. 759 - 767
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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