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DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)

Published online by Cambridge University Press:  11 April 2014

Y.J. Wang ,
Z.H. Li ,
S.F. Zhang ,
Z. Varadínová ,
F. Jiang ,
Z. Kučerová ,
V. Stejskal ,
G. Opit ,
Y. Cao  and
F.J. Li
Y.J. Wang
Affiliation:
Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China
Z.H. Li*
Affiliation:
Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China
S.F. Zhang
Affiliation:
Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China
Z. Varadínová
Affiliation:
Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, Prague, Czech Republic Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Drnovská 507, Prague, Czech Republic
F. Jiang
Affiliation:
Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China
Z. Kučerová
Affiliation:
Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Drnovská 507, Prague, Czech Republic
V. Stejskal
Affiliation:
Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Drnovská 507, Prague, Czech Republic
G. Opit
Affiliation:
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA
Y. Cao
Affiliation:
Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing, China
F.J. Li
Affiliation:
Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing, China
*
*Author for correspondence Phone: +86 -10- 62731299 Fax: +86 -10- 62733404 E-mail: lizh@cau.edu.cn
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Abstract

Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.

Keywords

Cryptolestesstored-product pestsidentificationDNA barcodingCOI
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 4 , August 2014 , pp. 486 - 493
Copyright
Copyright © Cambridge University Press 2014 

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