Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-14T06:55:13.606Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and expression patterns of candidate carboxylesterases in Carposina sasakii Matsumura (Lepidoptera: Carposinidae), an important pest of fruit trees

Published online by Cambridge University Press:  07 June 2022

Jia Li Open the ORCID record for Jia Li [Opens in a new window]  and
Long Zhang
Jia Li*
Affiliation:
Plant Protection College, Shenyang Agricultural University, Shenyang, China
Long Zhang
Affiliation:
College of Grassland Science and Technology, China Agricultural University, Beijing, China
*
Author for correspondence: Jia Li, Email: teacher135@sina.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Carposina sasakii Matsumura (Lepidoptera: Carposinidae) is an important pest of fruit trees in a large area of Asia. The adults mainly depend on olfaction to communicate with the environment, but the olfactory mechanism has not been well known. Odorant degrading enzymes (ODEs) are important olfactory proteins, which inactivate and degrade odorants to free odorant receptors for maintaining olfactory sensitivity. Carboxylesterases (CXEs) are considered to be a major group of moth ODEs. In this study, four candidate CXEs (CsasCXE1 ~ CsasCXE4) were identified by using head transcriptomic data from C. sasakii adult females and males. Sequence alignment showed conserved amino acid residues and their variations in C. sasakii CXEs. Phylogenetic analysis indicated the CXEs with the variations cluster well, and each C. sasakii CXE clusters in a clade with some of the other lepidopteran CXEs, with a high enough bootstrap value. Gene expression analysis revealed that CsasCXE2 and CsasCXE3 have similar tissue and sex expression patterns in C. sasakii adults. The two CXEs have relatively high expression levels in the heads and are expressed more abundantly in the female heads than male heads. CsasCXE1 and CsasCXE4 are expressed at higher levels in the male heads than female heads, but not dominantly expressed in the heads among the different tissues. Whether these CXEs function as ODEs remains to be further researched. This study laid the foundation for exploring functions of C. sasakii CXEs.

Keywords

Carboxylesteraseexpression patternCarposina sasakiitranscriptomic data
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 4 , August 2022 , pp. 567 - 573
Check for updates
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Fleischer, J, Pregitzer, P, Breer, H and Krieger, J (2018) Access to the odor world: olfactory receptors and their role for signal transduction in insects. Cellular and Molecular Life Sciences 75, 485–508.CrossRefGoogle Scholar
Groot, AT, Dekker, T and Heckel, DG (2016) The genetic basis of pheromone evolution in moths. Annual Review of Entomology 61, 99–117.CrossRefGoogle ScholarPubMed
Han, KS, Jung, JK, Choi, KH, Lee, SW and Boo, KS (2000) Sex pheromone composition and male trapping of the peach fruit moth, Carposina sasakii (Matsumura) (Lepidoptera: Carposinidae) in Korea. Journal of Asia-Pacific Entomology 3, 83–88.CrossRefGoogle Scholar
Hemingway, J, Hawkes, NJ, McCarroll, L and Ranson, H (2004) The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology 34, 653–665.CrossRefGoogle ScholarPubMed
Huang, X, Liu, L, Su, X and Feng, J (2016) Identification of biotransformation enzymes in the antennae of codling moth Cydia pomonella. Gene 580, 73–79.CrossRefGoogle ScholarPubMed
Ishida, Y and Leal, WS (2005) Rapid inactivation of a moth pheromone. Proceedings of the National Academy of Sciences of the United States of America 102, 14075–14079.CrossRefGoogle ScholarPubMed
Jones, BR and Bancroft, HR (1986) Distribution and probable physiological role of esterases in reproductive, digestive and fat-body tissues of the adult cotton boll weevil, Anthonomus grandis Boh. Biochemical Genetics 24, 499–508.CrossRefGoogle ScholarPubMed
Leal, WS (2013) Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annual Review of Entomology 58, 373–391.CrossRefGoogle ScholarPubMed
Li, L, Zhang, S, Zhang, A, Zhou, X, Men, X, Zhuang, Q and Yu, Y (2013) Biological characteristics of Beauveria bassiana and its virulence to overwintering larvae of Carposina niponensis Walsingham. Chinese Journal of Biological Control 29, 318–323.Google Scholar
Li, J, Wang, X and Zhang, L (2019) Identification of putative odorant binding proteins in the peach fruit borer Carposina sasakii (Lepidoptera: Carposinidae) by transcriptome analysis and their expression profile. Biochemical and Biophysical Research Communications 508, 1024–1030.CrossRefGoogle ScholarPubMed
Liu, S, Gong, ZJ, Rao, XJ, Li, MY and Li, SG (2015) Identification of putative carboxylesterase and glutathione S-transferase genes from the antennae of the Chilo suppressalis (Lepidoptera: Pyralidae). Journal of Insect Science 15, 1–8.CrossRefGoogle Scholar
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCt method. Methods (San Diego, Calif.) 25, 402–408.CrossRefGoogle Scholar
Oakeshott, JG, Claudianos, C, Campbell, PM, Newcomb, RD and Russell, R (2005) Biochemical genetics and genomics of insect esterases. In Gilbert, LI, Iatrou, K and Gill, SS (eds), Comprehensive Molecular Insect Science. London: Elsevier, pp. 309–361.CrossRefGoogle Scholar
Pelosi, P, Zhou, JJ, Ban, LP and Calvello, M (2006) Soluble proteins in insect chemical communication. Cellular and Molecular Life Sciences 63, 1658–1676.CrossRefGoogle ScholarPubMed
Pelosi, P, Iovinella, I, Zhu, J, Wang, G and Dani, FR (2018) Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects. Biological Reviews 93, 184–200.CrossRefGoogle ScholarPubMed
Steiner, C, Chertemps, T and Maïbèche, M (2019) Diversity of biotransformation enzymes in insect antennae: possible roles in odorant inactivation and xenobiotic processing. In Picimbon, JF (ed.), Olfactory Concepts of Insect Control – Alternative to Insecticides. Cham, Switzerland: Springer, pp. 115–145.CrossRefGoogle Scholar
Sun, Y and Wang, H (2015) Electroantennograms activities of Carposina sasakii Matsumura to volatiles of apples. Liaoning Agricultural Sciences 11–13.Google Scholar
Vogt, RG and Riddiford, LM (1981) Pheromone binding and inactivation by moth antennae. Nature 293, 161–163.CrossRefGoogle ScholarPubMed
Wang, X, Wang, H, Lv, S, Yang, F and Zhang, Z (2011) Study on the host selection process of Carposina niponensis oviposition. Hubei Agricultural Sciences 50, 2236–2239.Google Scholar
Ye, J, Mang, D, Kang, K, Chen, C, Zhang, X, Tang, Y, Purba, ER, Song, L, Zhang, QH and Zhang, L (2021) Putative carboxylesterase gene identification and their expression patterns in Hyphantria cunea (Drury). PeerJ 9, e10919.CrossRefGoogle ScholarPubMed
Younus, F, Chertemps, T, Pearce, SL, Pandey, G, Bozzolan, F, Coppin, CW, Russell, RJ, Maïbèche-Coisne, M and Oakeshott, JG (2014) Identification of candidate odorant degrading gene/enzyme systems in the antennal transcriptome of Drosophila melanogaster. Insect Biochemistry and Molecular Biology 53, 30–43.CrossRefGoogle ScholarPubMed
Zhang, YN, Xia, YH, Zhu, JY, Li, SY and Dong, SL (2014) Putative pathway of sex pheromone biosynthesis and degradation by expression patterns of genes identified from female pheromone gland and adult antenna of Sesamia inferens (Walker). Journal of Chemical Ecology 40, 439–451.CrossRefGoogle ScholarPubMed
Zhang, YN, Li, JB, He, P, Sun, L, Li, ZQ, Fang, LP, Ye, ZF, Deng, DG and Zhu, XY (2016) Molecular identification and expression patterns of carboxylesterase genes based on transcriptome analysis of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Journal of Asia-Pacific Entomology 19, 989–994.CrossRefGoogle Scholar
Zhang, Z, Li, X, Xue, Y, Zhao, Z, Li, J and Ma, R (2017 a) Increased trapping efficiency for the peach fruit moth Carposina sasakii (Matsumura) with synthetic sex pheromone. Agricultural and Forest Entomology 19, 424–432.CrossRefGoogle Scholar
Zhang, YX, Wang, WL, Li, MY, Li, SG and Liu, S (2017 b) Identification of putative carboxylesterase and aldehyde oxidase genes from the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae). Journal of Asia-Pacific Entomology 20, 907–913.CrossRefGoogle Scholar
Supplementary material: File

Li and Zhang supplementary material

Tables S1-S3

Download Li and Zhang supplementary material(File)
File 83.5 KB