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A technique for the topical application of poisons to non-anaesthetised house-flies for knockdown assessments.

Published online by Cambridge University Press:  10 July 2009

R. M. Sawicki
R. M. Sawicki
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts.
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Extract

An apparatus and technique are described for handling, immobilising by suction, and individually dosing house-flies, Musca domestica L. The apparatus consists of a suction platform connected to a vacuum cleaner, a specially designed cage and a measured-drop apparatus. The suction platform consists essentially of a circle of terylene gauze, on to which the flies are drawn from the cage and held by suction during treatment. The cage can be made to contract, so that the flies may be crowded together near the special emergence hole through which they are to be withdrawn. The technique avoids the use of cooling and anaesthetics, which affect the metabolism of the flies, and is therefore especially suitable for measuring knockdown, although it can equally be used to estimate toxicity. Some factors likely to influence the results are examined and discussed, and an example is given of two experiments with house-flies, to show that the method gives reproducible results.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 51 , Issue 4 , January 1961 , pp. 715 - 722
Copyright
Copyright © Cambridge University Press 1961

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