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Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)

Published online by Cambridge University Press:  28 March 2016

M. Nenadić ,
M. Soković ,
J. Glamočlija ,
A. Ćirić ,
V. Perić-Mataruga ,
V. Tešević ,
L. Vujisić ,
M. Todosijević ,
N. Vesović  and
S. Ćurčić
M. Nenadić
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
M. Soković
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
J. Glamočlija
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
A. Ćirić
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
V. Perić-Mataruga
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
V. Tešević
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
L. Vujisić
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
M. Todosijević
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
N. Vesović
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
S. Ćurčić*
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
*
*Author for correspondence Tel: +381 11 2187266 Fax: +381 11 2638500 E-mail: srecko@bio.bg.ac.rs
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Abstract

The antimicrobial activity of the pygidial gland secretion released by adult individuals of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828), applying microdilution method with the aim to detect minimal inhibitory concentration, minimal bactericidal concentration and minimal fungicidal concentration, has been investigated. In addition, morphology of the pygidial glands is observed. We have tested 16 laboratory and clinical strains of human pathogens – eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples have showed antimicrobial properties against all strains of treated bacteria and fungi. Micrococcus flavus proved to be more resistant compared with other bacterial strains. More significant antimicrobial properties of the secretion are observed against Escherichia coli, which proved to be the most sensitive bacteria. Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and Penicillium verrucosum var. cyclopium the most sensitive micromycetes. Commercial antibiotics Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied as positive controls, showed higher antibacterial properties for all bacterial and fungal strains, except for P. ochrochloron, which proved to be more resistant on Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart from the role in ecological aspects, the antimicrobial properties of the tested secretion possibly might have medical significance in the future.

Keywords

Laemostenus (Pristonychus) punctatuspygidial gland secretionantibacterial and antifungal activityhuman pathogensmicrodilutionmorphology
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 4 , August 2016 , pp. 474 - 480
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Copyright
Copyright © Cambridge University Press 2016 

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References

Beutel, R. (1997) Phylogenese and Evolution der Coleoptera (Insecta), insbesondere der Adephaga. Abhandlungen des Naturwissenschaftlichen Vereins in Hamburg 31, 1–164.Google Scholar
Blum, M.S. (1981) Chemical Defenses of Arthropods. New York, Academic Press.Google Scholar
Bonacci, T., Brandmayr, P., Zetto, T., Perrotta, I.D., Guarino, S., Peri, E. & Colazza, S. (2011) Volatile compounds released by disturbed and undisturbed adults of Anchomenus dorsalis (Coleoptera, Carabidae, Platynini) and structure of the pygidial gland. ZooKeys 81, 13–25.Google Scholar
Daouk, K.D., Dagher, M.S. & Sattout, J.E. (1995) Antifungal activity of the essential oil of Origanum syriacum L. Journal of Food Protection 58, 1147–1149.Google Scholar
Degenkolb, T., Düring, R.A. & Vilcinskas, A. (2011) Secondary metabolites released by the burying beetle Nicrophorus vespilloides: chemical analyses and possible ecological functions. Journal of Chemical Ecology 37, 724–735.Google Scholar
Desbois, A.P. & Smith, V.J. (2010) Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology 85, 1629–1642.Google Scholar
Dettner, K. (1985) Ecological and phylogenetic significance of defensive compounds from pygidial glands of Hydradephaga (Coleoptera). Proceedings of the Academy of Natural Sciences of Philadelphia 137, 156–171.Google Scholar
Forsyth, D.J. (1972) The structure of the pygidial defence glands of Carabidae (Coleoptera). Transactions of the Zoological Society of London 32, 249–309.Google Scholar
Francke, W. & Dettner, K. (2005) Chemical signalling in beetles. pp. 85–166 in Schulz, S. (Ed.) The Chemistry of Pheromones and Other Semiochemicals II. Topics in Current Chemistry, Volume 240. Berlin-Heidelberg, Springer-Verlag.Google Scholar
Fujita, K., Fujita, T. & Kubo, I. (2007) Anethole, a potential antimicrobial synergist, converts a fungistatic dodecanol to a fungicidal agent. Phytotherapy Research 21, 47–51.Google Scholar
Ghilarov, M.S. (1958) Biologhicheski aktivnye veshchestva, vydeljaemye nasekomymi. Uspekhi sovremennoi biologii 2, 208–216.Google Scholar
Giglio, A., Brandmayr, P., Talarico, F. & Zetto, T. (2011) Current knowledge on exocrine glands in carabid beetles: structure, function and chemical compounds. ZooKeys 100, 193–201.Google Scholar
Glassman, H.N. (1948) Surface active agents and their application in bacteriology. Bacteriological Reviews 12, 105–148.Google Scholar
Hanel, H. & Raether, W. (1988) A more sophisticated method of determining the fungicidal effect of water-insoluble preparations with a cell harvester, using miconazole as an example. Mycoses 31, 148–154.Google Scholar
Hoback, W.W., Bishop, A.A., Kroemer, J., Scalzitti, J. & Shaffer, J.J. (2004) Differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology. Journal of Chemical Ecology 30, 719–729.Google Scholar
Kabara, J.J., Swieczkowski, D.M., Conley, A.J. & Truant, J.P. (1972) Fatty acids and derivatives as antimicrobial agents. Antimicrobial Agents and Chemotherapy 2, 23–28.Google Scholar
Kovac, D. & Maschwitz, U. (1990) Secretion-grooming in aquatic beetles (Hydradephaga): a chemical protection against contamination of the hydrofuge respiratory region. Chemoecology 1, 131–138.Google Scholar
Lečić, S., Ćurčić, S., Vujisić, L., Ćurčić, B., Ćurčić, N., Nikolić, Z., Anđelković, B., Milosavljević, S., Tešević, V. & Makarov, S. (2014) Defensive secretions in three ground-beetle species (Insecta: Coleoptera: Carabidae). Annales Zoologici Fennici 51, 285–300.CrossRefGoogle Scholar
Makarov, S.E., Dimkić, I.Z., Antić, D.Ž., Vujisić, L.V., Stević, T.R., Mitić, B.M., Tomić, V.T., Ilić, B.S., Ćurčić, B.P.M. & Stanković, S.M. (2014) Pachyiulus hungaricus (Karsch, 1881) (Myriapoda, Diplopoda, Julidae) – a model-system for semiochemical analysis and antimicrobial testing. p. 51 in Book of Abstracts of the 16th International Congress of Myriapodology, Olomouc, 20–25 July 2014 Olomouc, Institute of Soil Biology, BC ASCR & Faculty of Science, Palacký University.Google Scholar
Pavan, M. (1949) Ricerche sugli antibiotici di origine animale. Nota riassuntiva. La Ricerca Scientifica 19, 1011–1017.Google Scholar
Raftar, M., Jalilian, F.A., Abdulamir, A.S., Ghafurian, S., Sekawi, Z., Son, R. & Bakar, F.B. (2012) Antibacterial activity of organic acids on Salmonella and Listeria . The Asian International Journal of Life Sciences 21, 13–30.Google Scholar
Raftari, M., Jalilian, F.A., Abdulamir, A.S., Son, R., Sekawi, Z. & Fatimah, A.B. (2009) Effect of organic acids on Escherichia coli O157:H7 and Staphylococcus aureus contaminated meat. The Open Microbiology Journal 3, 121–127.Google Scholar
Roach, B., Eisner, T. & Meinwald, J. (1990) Defense mechanisms of arthropods. 83. α- and β-necrodol, novel terpenes from a carrion beetle (Necrodes surinamensis, Silphidae, Coleoptera). The Journal of Organic Chemistry 55, 4047–4051.Google Scholar
Roncadori, R.W., Dufey, S.S. & Blum, M.S. (1985) Antifungal activity of defensive secretions of certain millipedes. Mycologia 77, 185–191.Google Scholar
Rossini, C., Attygalle, A.B., Gonzalez, A., Smedley, S.R., Eisner, M., Meinwald, J. & Eisner, T. (1997) Defensive production of formic acid (80%) by a carabid beetle (Galerita lecontei). Proceedings of the National Academy of Sciences of the United States of America 94, 6792–6797.Google Scholar
Thiele, H.U. (1977) Carabid Beetles in Their Environments. A Study on Habitat Selection by Adaptations in Physiology and Behaviour. Berlin-Heidelberg, Springer-Verlag.Google Scholar
Togashi, N., Shiraishi, A., Nishizaka, M., Matsuoka, K., Endo, K., Hamashima, H. & Inoue, Y. (2007) Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus . Molecules 12, 139–148.CrossRefGoogle ScholarPubMed
Xylander, W.E.R. (2009) Antibacterial substances and characteristics of the haemolymph of Chilopoda and Diplopoda (Myriapoda, Arthropoda). Soil Organisms 81, 413–429.Google Scholar
Vesović, N., Ćurčić, S., Vujisić, L., Nenadić, M., Krstić, G., Perić-Mataruga, V., Milosavljević, S., Antić, D., Mandić, B., Petković, M., Vučković, I., Marković, Đ., Vrbica, M., Ćurčić, B. & Makarov, S. (2015) Molecular diversity of compounds from pygidial gland secretions of cave-dwelling ground beetles: the first evidence. Journal of Chemical Ecology 41, 533–539.CrossRefGoogle ScholarPubMed