Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T11:42:45.992Z Has data issue: false hasContentIssue false
  • English
  • Français

Biochemical mechanisms of organophosphate resistance in Rhyzopertha dominica (Coleoptera: Bostrichidae) populations from the United States and Brazil

Published online by Cambridge University Press:  10 July 2009

R. N. C. Guedes ,
S. Kambhampati ,
B. A. Dover  and
K. Y. Zhu
R. N. C. Guedes
Affiliation:
Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG 36571.000, Brazil:
S. Kambhampati
Affiliation:
Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA
B. A. Dover
Affiliation:
Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA
K. Y. Zhu*
Affiliation:
Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA
*
* Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Abstract

Four possible biochemical mechanisms of organophosphate resistance were examined in populations of Rhyzopertha dominica (Fabricius) collected from eight locations in the states of Minas Gerais and São Paulo in Brazil and from seven locations in northeast Kansas, United States. Results from insecticide bioassays with synergists (triphenyl phosphate, diethylmaleate, and piperonyl butoxide) suggested that metabolic detoxification mechanisms might be involved in organophosphate resistance in some of the populations of R. dominica. However, overall low synergism, non-synergism or even antagonism presented by these synergists in all 15 resistant populations suggested that carboxylesterases, cytochrome P450 monooxygenases and glutathione S-transferases were not likely to play a major role conferring organophosphate resistance in R. dominica. This hypothesis was strongly supported by our biochemical studies showing no significant difference in these enzyme activities, as well as cytochrome b5, P420, and P450 levels between the susceptible and resistant populations. In contrast, all resistant populations of R. dominica showed higher acetylcholinesterase activity than the susceptible population both in the presence and absence of malaoxon. Furthermore, acetylcholinesterase from the resistant populations was less sensitive to inhibition by malaoxon than that from the susceptible. These results suggested that quantitative and qualitative changes in acetylcholinesterase might contribute to organophosphate resistance in these populations of R. dominica.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 87 , Issue 6 , December 1997 , pp. 581 - 586
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anon. (1987) SAS user's guide: statistics, version 5.15. Cary, North Carolina, SAS Institute.Google Scholar
Argentine, J.A., Zhu, K.Y., Lee, S.H. & Clark, J.M. (1994) Biochemical mechanisms of azinphosmethyl resistance in isogenic strains of Colorado potato beetle. Pesticide Biochemistry and Physiology 48, 63–78.CrossRefGoogle Scholar
Badmin, J.S. (1990) IRAC survey of resistance of stored grain pests: results and progress. pp. 973–981in Fleurat-Lessard, & Ducom, P. (Eds) 5th international working conference on stored-product protection.Bordeaux, France,IRAC (Proceedings, Institut National de la Recherche Agronomique).Google Scholar
Beeman, R.W. & Wright, V.F. (1990) Monitoring for resistance to chlorpyrifos-methyl, pirimiphos-methyl and malathion in Kansas populations of stored-product insects. Journal of the Kansas Entomological Society 63, 385–392.Google Scholar
Bernard, C.B. & Philogène, B.J.R. (1993) Insecticide synergists: role, importance, and perspectives. Journal of Toxicology and Environmental Health 38, 199–223.CrossRefGoogle Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Champ, B.R. & Dyte, C.E. (1976) FAO global survey of pesticide susceptibility of stored grain pests. 356 pp. Rome, FAO/UN.Google Scholar
Ellman, G.L., Courtney, K.D., Andres, V. Jr. & Featherstone, R.M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7, 88–95.CrossRefGoogle ScholarPubMed
Guedes, R.N.C. (1990) Resistência a inseticidas: desafio para o controle de pragas de grãos armazenados. Seiva 50, 24–29.Google Scholar
Guedes, R.N.C., Lima, J.O.G., Santos, J.P. & Cruz, C.D. (1995) Resistance to DDT and pyrethroids in Brazilian populations of Sitophilus zeamais Motsch. (Coleoptera: Cucurlionidae). Journal of Stored Products Research 31, 145–150.CrossRefGoogle Scholar
Guedes, R.N.C., Dover, B.A. & Kambhampati, S. (1996) Resistance to chlorpyrifos-methyl, pirimiphos-methyl, and malathion in Brazilian and US populations of Rhyzopertha dominica (Coleoptera: Bostrichidae). Journal of Economic Entomology 89, 27–32.CrossRefGoogle Scholar
Guedes, R.N.C., Kambhampati, S. & Dover, B.A. (1997) Allozyme variation among Brazilian and US Populations of Rhyzopertha dominica resistant to insecticides. Entomologia Experimentalis et Applicata (in press).CrossRefGoogle Scholar
Haliscak, J.P. & Beeman, R.W. (1983) Status of malathion resistance in five genera of beetles infesting farm-stored corn, wheat, and oats in the United States. Journal of Economic Entomology 76, 717–722.CrossRefGoogle Scholar
Hansen, L.G. & Hodgson, E. (1971) Biochemical characteristics of insect microsomes: N- and O-demethylation. Biochemical Pharmacology 20, 1569–1578.CrossRefGoogle ScholarPubMed
Jesudason, P., Levi, P.E., Weiden, M. & Roe, R.M. (1988) Developmental changes in the microsomal monooxygenase system and the in vivo metabolism of aldrin in larvae of the Mexican bean beetle (Coleoptera: Coccinellidae). Journal of Economic Entomology, 81, 1598–1605.CrossRefGoogle Scholar
Kao, L.R., Motoyama, N. & Dauterman, W.C. (1985) Multiple forms of esterases in mouse, rat, and rabbit liver, and their role in hydrolysis of organophosphorus and pyrethroid insecticides. Pesticide Biochemistry and Physiology 23, 66–73.CrossRefGoogle Scholar
Karunaratne, K.M. & Plapp, F.W. Jr. (1993) Biochemistry and genetics of thiodicarb resistance in the house fly (Diptera: Muscidae). Journal of Economic Entomology 86, 258–264.CrossRefGoogle ScholarPubMed
Omura, T. & Sato, R. (1964) The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. Journal of Biological Chemistry 239, 2370–2378.CrossRefGoogle ScholarPubMed
Pacheco, I.A., Sartori, M.R. & Bolonhezi, S. (1990) Resistance to malathion, primiphos-methyl and fenitrothion in Coleoptera from stored grains. pp. 1029–1037in FleurratLessard, F. & Ducom, P. (Eds) 5th international working conference on stored-product protection.Bordeaux, France,INRA (Proceedings, Institut National de la Recherche Agronomique).Google Scholar
Raffa, K.F. & Priester, T.M. (1985) Synergists as research tools and control agents in agriculture. Journal of Agricultural Entomology 2, 27–45.Google Scholar
Rees, D.P. (1996) Coleoptera. pp. 1–39, in Subramanyam, B. & Hagstrum, D.W. (Eds) Integrated management of insects in stored products. New York, Marcel Dekker.Google Scholar
Roush, R.T. & Miller, G.L. (1986) Considerations for design of insecticide resistance monitoring programs. Journal of Economic Entomology 79, 293–298.CrossRefGoogle Scholar
Subramanyam, B. & Hagstrum, D.W. (1996) Resistance mesurement and management. pp. 331–397, in Subramanyam, B. & Hagstrum, D.W. (Eds) Integrated management of insects in stored products. New York, Marcel Dekker.Google Scholar
Van Asperen, K. (1962) A study of housefly esterases by means of a sensitive colorimetric method. Journal of Insect Physiology 8, 401–416.CrossRefGoogle Scholar
Yu, S.J. (1982) Host plant induction of glutathione S-transferases in the fall armyworm. Pesticide Biochemistry and Physiology 18, 101–106.CrossRefGoogle Scholar
Yu, S.J. (1984) Interactions of allelochemicals with detoxification enzymes of insecticide-susceptible and resistant fall armyworms. Pesticide Biochemistry and Physiology 22, 60–68.CrossRefGoogle Scholar
Yu, S.J., Robinson, F.A. & Nation, J.L. (1984) Detoxification capacity in the honey bee, Apis mellifera L. Pesticide Biochemistry and Physiology 22, 360–368.CrossRefGoogle Scholar
Zettler, J.L. & Cuperus, G.W. (1990) Pesticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) in wheat. Journal of Economic Entomology 83, 1677–1681.CrossRefGoogle Scholar