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Can behavioural differences in Platypus cylindrus (Coleoptera: Platypodinae) from Portugal and Tunisia be explained by genetic and morphological traits?

Published online by Cambridge University Press:  21 July 2015

A. Bellahirech ,
M.L. Inácio ,
F. Nóbrega ,
J. Henriques ,
L. Bonifácio ,
E. Sousa  and
M.L. Ben Jamâa
A. Bellahirech*
Affiliation:
National Research Institute of Rural Engineering, Water and Forests, B.P. N°10, 2080, Ariana, Tunisia
M.L. Inácio
Affiliation:
Instituto Nacional de Investigação Agrária e Veterinária, I.P. Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal
F. Nóbrega
Affiliation:
Instituto Nacional de Investigação Agrária e Veterinária, I.P. Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal
J. Henriques
Affiliation:
Instituto Nacional de Investigação Agrária e Veterinária, I.P. Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal
L. Bonifácio
Affiliation:
Instituto Nacional de Investigação Agrária e Veterinária, I.P. Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal
E. Sousa
Affiliation:
Instituto Nacional de Investigação Agrária e Veterinária, I.P. Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal
M.L. Ben Jamâa
Affiliation:
National Research Institute of Rural Engineering, Water and Forests, B.P. N°10, 2080, Ariana, Tunisia
*
*Author for correspondence Phone: (+216) 71 230 039 Fax: (+216) 71717951 E-mail: amany21@hotmail.fr
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Abstract

Platypus cylindrus is an important wood borer of cork oak trees (Quercus suber) in the Mediterranean region, namely Portugal, Morocco and Algeria where its presence has drastically increased in the past few decades. On the contrary, the insect is not a relevant pest in Tunisia. The aim of this work is to analyze morphological and genetic differences among Tunisian and Portuguese populations in order to understand their role in the diverse population dynamics (e.g., aggressiveness) of the insect. The information could be used as a novel tool to implement protective measures. Insects were collected from cork oak stands in Tunisia (Ain Beya, Babouch and Mzara) and Portugal (Chamusca and Crato). Morphological traits of female and male mycangial pits were determined, using scanning electron microscopy but no significant differences were found. Genetic differences were analyzed using nuclear (internal simple sequence repeat polymerase chain reaction) and mitochondrial (cytochrome oxidase I (COI)) molecular markers. The results showed a very low level of intraspecific polymorphism and genetic diversity. The alignment of COI sequences showed high percentage of identical sites (99%) indicating a very low variation in nucleotide composition. Other variables related with the ecology of the insect and its associated fungi must be studied for a better understanding of the differences in the insect population's dynamic in Mediterranean countries.

Keywords

ambrosia beetlecork oakcytochrome oxidase IPlatypus cylindrusinternal simple sequence repeat polymerase chain reaction (ISSR-PCR)
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 1 , February 2016 , pp. 1 - 8
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Copyright
Copyright © Cambridge University Press 2015 

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