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Clones of pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae) distinguished using genetic markers, differ in their damaging effect on a resistant alfalfa cultivar

Published online by Cambridge University Press:  02 March 2010

R. Bournoville ,
J.-C. Simon ,
I. Badenhausser ,
C. Girousse ,
T. Guilloux  and
S. André
R. Bournoville*
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
J.-C. Simon
Affiliation:
LIMR Biologie des Population et des Organismes appliquée à la Protection des Plantes, INRA, B.P. 29, 35650 Le Rheu, France
I. Badenhausser
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
C. Girousse
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
T. Guilloux*
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
S. André
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
*
*Fax: (33) 05 49 55 60 88 E-mail: bourno@lusignan.inra.fr
*Fax: (33) 05 49 55 60 88 E-mail: bourno@lusignan.inra.fr
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Abstract

CUF 101, a resistant cultivar of alfalfa, was exposed to 15 clones of Acyrthosiphon pisum Harris collected from alfalfa fields in three regions of France (east, south, central west) to determine whether the level of resistance varied across the different clones. The survival of alfalfa seedlings infested at the cotyledon stage was assessed using a standardized method. Although no difference in seedling mortality was detected between clones grouped by region, there was a significant variation among the 15 pea aphid clones. In particular, two clones of southern origin were more aggressive. In addition, the different pea aphid clones were characterized using allozyme and RAPD-PCR markers. Among the 15 clones, seven allozyme genotypes (plus one when adding colour polymorphism) and 12 RAPD-PCR genotypes were distinguished. The two southern clones differing by their aggressiveness on the resistant alfalfa belonged to the same allozyme and RAPD genotype which was distinct from the other pea aphid clones. Our results reinforce the need to take into account aphid genetic diversity in breeding programmes for resistance in cultivated plants.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 90 , Issue 1 , February 2000 , pp. 33 - 39
Copyright
Copyright © Cambridge University Press 2000

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