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Development of DNA probes for the identification of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex

Published online by Cambridge University Press:  10 July 2009

Maya B. Gunasekera ,
B.G.D.N.K. de Silva ,
W. Abeyewickreme ,
S.K. Subbarao ,
H.G. Nandadasa  and
E.H. Karunanayake
Maya B. Gunasekera*
Affiliation:
Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka
B.G.D.N.K. de Silva
Affiliation:
Faculty of Applied Science, University of Sri Jayewardenepura, Sri Lanka
W. Abeyewickreme
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Colombo, Sri Lanka
S.K. Subbarao
Affiliation:
Malaria Research Centre, Delhi, India
H.G. Nandadasa
Affiliation:
Faculty of Applied Science, University of Sri Jayewardenepura, Sri Lanka
E.H. Karunanayake
Affiliation:
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka
*
Dr Maya B. Gunasekera, Department of Chemistry, Faculty of Science, University of Colombo, Colombo 3, Sri Lanka.
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Abstract

Three highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu lato. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 85 , Issue 3 , September 1995 , pp. 345 - 353
Copyright
Copyright © Cambridge University Press 1995

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