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Digestive α-amylases from Tecia solanivora larvae (Lepidoptera: Gelechiidae): response to pH, temperature and plant amylase inhibitors

Published online by Cambridge University Press:  01 July 2008

A. Valencia-Jiménez ,
J.W. Arboleda V ,
A. López Ávila  and
M.F. Grossi-de-Sá
A. Valencia-Jiménez*
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, PqEB, W5 Norte Final, Brasília – DF, 70770-900, Brasil Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, Brasília – DF, 70910-900, Brasil Facultad de Ciencias Agropecuarias, Departamento de Fitotecnia, Universidad de Caldas, Calle 65#26-10, Manizales, Colombia
J.W. Arboleda V
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, PqEB, W5 Norte Final, Brasília – DF, 70770-900, Brasil Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, Brasília – DF, 70910-900, Brasil
A. López Ávila
Affiliation:
Corpoica, Tibaitatá, Bogotá, Colombia
M.F. Grossi-de-Sá
Affiliation:
Embrapa Recursos Genéticos e Biotecnologia, PqEB, W5 Norte Final, Brasília – DF, 70770-900, Brasil
*
*Author for correspondence Fax: +57-68-781545 E-mail: avalbioquimica@yahoo.com
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Abstract

The biochemical properties of the digestive alpha-amylase from Tecia solanivora larvae, an important and invasive insect pest of potato (Solanum tuberosum), were studied. This insect has three major digestive α-amylases with isoelectric points 5.30, 5.70 and 5.98, respectively, which were separated using native and isoelectric focusing gels. The alpha-amylase activity has an optimum pH between 7.0 and 10.0 with a peak at pH 9.0. The enzymes are stable when heated to 50°C and were inhibited by proteinaceous inhibitors from Phaseolus coccineus (70% inhibition) and P. vulgaris cv. Radical (87% inhibition) at pH 6.0. The inhibitors present in an amaranth hybrid inhibited 80% of the activity at pH 9.0. The results show that the alpha-amylase inhibitor from amaranth seeds may be a better candidate to make genetically-modified potatoes resistant to this insect than inhibitors from common bean seeds.

Keywords

α-AmylaseTecia solanivoraamylase inhibitorspotato
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 6 , December 2008 , pp. 575 - 579
Copyright
Copyright © 2008 Cambridge University Press

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