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Effect of antibiotic on survival and development of Spodoptera litura (Lepidoptera: Noctuidae) and its gut microbial diversity

Published online by Cambridge University Press:  24 February 2016

A. Thakur ,
P. Dhammi ,
H.S. Saini  and
S. Kaur
A. Thakur
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar (Punjab), 143005, India
P. Dhammi
Affiliation:
Department of Microbiology, Guru Nanak Dev University, Amritsar (Punjab), 143005, India
H.S. Saini
Affiliation:
Department of Microbiology, Guru Nanak Dev University, Amritsar (Punjab), 143005, India
S. Kaur*
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar (Punjab), 143005, India
*
*Author for correspondence Phone: +91-0183-2258802-09 Ext. 3397 Fax: +91-0183-2258819/2258820 E-mail: sanehsaini@gmail.com
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Abstract

Addition of antibiotics to artificial diets of insects is a key component in the rearing of insects in the laboratory. In the present study an antimicrobial agent, streptomycin sulphate was tested for its influence on survival and fitness of Spodoptera litura (Fabricus) (Lepidoptera: Noctuidae) as well as its gut microbial diversity. The antibiotic did not adversely affect the survival of S. litura. Faster growth of larvae was recorded on diet amended with different concentrations of streptomycin sulphate (0.03, 0.07 and 0.15%) as compared to diet without streptomycin sulphate. The overall activity of various digestives enzymes increased on S+ diet while the activity of detoxifying enzymes significantly decreased. In addition, alteration in microbial diversity was found in the gut of S. litura larvae fed on diet supplemented with antibiotic (S+) and without antibiotic (S−).

Keywords

antibioticSpodoptera liturafitness of insectenzyme activitygut microflora
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 3 , June 2016 , pp. 387 - 394
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Copyright
Copyright © Cambridge University Press 2016 

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