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F2 screen for resistance to a Bacillus thuringiensis-maize hybrid in the sugarcane borer (Lepidoptera: Crambidae)

Published online by Cambridge University Press:  04 October 2007

F.N. Huang ,
B.R. Leonard  and
D.A. Andow
F.N. Huang*
Affiliation:
Department of Entomology, 404 Life Sciences Building, Louisiana State University AgCenter, Baton Rouge, Louisiana70803, USA
B.R. Leonard
Affiliation:
Macon Ridge Research Station, Louisiana State University AgCenter, WinnsboroLA 71295, USA
D.A. Andow
Affiliation:
Department of Entomology, University of Minnesota, St. Paul, MN55108, USA
*
*Fax: +1225 578 1634 E-mail: fhuang@agcenter.lsu.edu
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Abstract

A novel F2 screening technique was developed for detecting resistance in sugarcane borer, Diatraea saccharalis (F.), to transgenic Bacillus thuringiensis (Bt)-maize expressing the Cry1Ab insecticidal protein. The F2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening F2 neonates for survival on Bt-maize leaf tissues; and (iv) confirming resistance on commercial Bt-maize plants. With the F2 screening method, 213 iso-line families of D. saccharalis were established from field collections in northeast Louisiana, USA and were screened for Bt resistance. One family was confirmed to carry a major Bt resistance allele(s). In a laboratory bioassay, larval mortality of the Bt-resistant D. saccharalis on Bt-maize leaf tissues was significantly lower than that of a Bt-susceptible strain. This Bt-resistant D. saccharalis population is the first corn stalk borer species that has completed larval development on commercial Bt-maize. The F2 screening protocol developed in this study could be modified for detecting Bt resistance alleles in other similar corn stalk borers, such as the European corn borer, Ostrinia nubilalis (Hübner), and the southwestern corn borer, D. grandiosella Dyar.

Keywords

Diatraea saccharalisBt-maizeresistance monitoringinsecticide resistance management
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 5 , October 2007 , pp. 437 - 444
Copyright
Copyright © Cambridge University Press 2007

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