Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T12:26:16.193Z Has data issue: false hasContentIssue false
  • English
  • Français

Factors affecting resistance to insecticides in house-flies, Musca domestica L. (Diptera: Muscidae). III. Relationship between the level of resistance to pyrethroids, control failure in the field and the frequency of gene kdr

Published online by Cambridge University Press:  10 July 2009

A. W. Farnham ,
Kate E. O'dell ,
I. Denholm  and
R. M. Sawicki
A. W. Farnham
Affiliation:
Department of Insecticides and Fungicides, Rothamsted Experimental Station, Harpenden, Herts., UK
Kate E. O'dell
Affiliation:
Department of Insecticides and Fungicides, Rothamsted Experimental Station, Harpenden, Herts., UK
I. Denholm
Affiliation:
Department of Insecticides and Fungicides, Rothamsted Experimental Station, Harpenden, Herts., UK
R. M. Sawicki
Affiliation:
Department of Insecticides and Fungicides, Rothamsted Experimental Station, Harpenden, Herts., UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Tolerance of bioresmethrin, permethrin, deltamethrin and the natural pyrethrins with and without synergists (piperonyl butoxide and propyl prop-2-ynyl phenylphosphonate), and the frequency of kdr homozygotes (flies surviving a discriminating dose of DDT+FDMC (1,1-bis(4-chlorophenyl)-2,2,2-trifluoroethanol)) was investigated in 42 strains of Musca domestica L. collected on British pig farms and in one Canadian strain. Although all field strains were at least 4–10 times more tolerant of synthetic pyrethroids at the LD50 than the standard susceptible Cooper strain, and 27 strains (26 British + 1 Canadian) had detectable levels of kdr, pyrethroids failed to control flies on only nine farms where 10% of flies or more were homozygous for kdr and/or the LD50s (in μg/female) in bioassays equalled or exceeded 0·1 for bioresmethrin, 0·12 for permethrin, 0·012 for deltamethrin or 1·1 for pyrethrins. These figures correspond to resistance factors (Cooper strain = 1) of 20, 15, 30 and 4, respectively. Regression analyses showed a close relationship between the LD50s to pyrethroids and the frequency of kdr homozygotes. Cross-resistance to trichlorfon, which in part results in the 4–10-fold increased tolerance to pyrethroids observed in strains lacking kdr, was insufficient to cause control failure. The quantification of the relationship between the frequency of an insecticide-resistant genotype, levels of resistance as measured by bioassays and control failure in the field provides the means of forewarning when control failure is likely to occur and of confirming resistance as the cause when it has taken place.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 74 , Issue 4 , December 1984 , pp. 581 - 589
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anon. (1981). Instructions for determining the susceptibility for resistance of adult mosquitos to organochlorine, organophosphate and carbamate insecticides—diagnostic tests.—12 pp. Geneva, Wld Hlth Org. (WHO/VBC/81.806).Google Scholar
Ball, H. J. (1981). Insecticide resistance—a practical assessment.—Bull. ent. Soc. Am. 27, 261–262.Google Scholar
Busvine, J. R. (1980). Recommended methods for measurement of pest resistance to pesticides.—132 pp. Rome, FAO.Google Scholar
Denholm, I., Farnham, A. W., O'Dell, K. & Sawicki, R. M. (1983). Factors affecting resistance to insecticides in house-flies, Musca domestica L. (Diptera: Muscidae). I. Long-term control with bioresmethrin of flies with strong pyrethroid-resistance potential.—Bull. ent. Res. 73, 481–489.CrossRefGoogle Scholar
Micks, D. W., Moon, W. B. & Mcneill, J. C. IV (1980). Malathion tolerance vs. resistance in Culex quinquefasciatus.—Mosquito News 40, 520–523.Google Scholar
Ross, G. J. S. (1980). MLP—maximum likelihood program.—100 pp. Harpenden, UK, Rothamsted Expl Stn.Google Scholar
Sawicki, R. M. & Denholm, I. (1984). Adaptation of insects to insecticides.—pp. 152–166in Evered, D. & Collins, J. M. (Eds.). Origins and development of adaptation.—273 pp. London. Pitman Books (Ciba Foundation Symposium No. 102).Google Scholar
Sawicki, R. M., Devonshire, A. L., Farnham, A. W., O'Dell, K. E., Moores, G. D. & Denholm, I. (1984). Factors affecting resistance to insecticides in house-flies, Musca domestica L. (Diptera: Muscidae). II. Close linkage on autosome 2 between an esterase and resistance to trichlorphon and pyrethroids.—Bull. ent. Res. 74, 197–206.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1967). Genetics of resistance to insecticides of the SKA strain of Musca domestica. I. Location of the main factors responsible for the maintenance of high DDT-resistance in diazinon-selected SKA flies.—Entomologia exp. appl. 10, 253–262.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1968). Genetics of resistance to insecticides of the SKA strain of Musca domestica. III. Location and isolation of the factors of resistance to dieldrin.—Entomologia exp. appl. 11, 133–142.CrossRefGoogle Scholar
Sawicki, R. M., Farnham, A. W., Denholm, I. & O'Dell, K. (1981). Housefly resistance to pyrethroids in the vicinity of Harpenden.—pp. 609–616in 1981 British Crop Protection Conference: Pests and Diseases (11th British Insecticide and Fungicide Conference). Volume 2.—pp. 363–672. Croydon, UK,Br. Crop Prot. Coun.Google Scholar