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Genetic Diversity and Differentiation among Laboratory and Field Populations of the Soybean Aphid, Aphis glycines

Published online by Cambridge University Press:  27 May 2010

A.P. Michel ,
W. Zhang  and
M.A. Rouf Mian
A.P. Michel*
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH44691, USA
W. Zhang
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH44691, USA
M.A. Rouf Mian
Affiliation:
USDA-ARS and Department of Horticulture and Crop Sciences, The Ohio State University, Wooster, OH44691, USA
*
*Author for correspondence Fax: +01 330-263-3686 E-mail: michel.70@osu.edu
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Abstract

The soybean aphid, Aphis glycines Matsumura, is a recent invasive pest of soybean in North America. Currently, much research is focused on developing and characterizing soybean cultivars expressing host-plant resistance. During the initial phases of host-plant resistance screening, many of these studies use soybean aphid laboratory populations. Previous studies in other systems have documented substantial differences among laboratory and field populations. Whether or not this pattern exists in A. glycines is unknown, but it is extremely important when estimating the level of selection and virulence to host-plant resistant soybeans. In this study, we used seven microsatellite markers to estimate and compare genetic diversity and differentiation among five laboratory and 12 field populations. Our results indicate that soybean aphid laboratory populations are severely lacking in genotypic diversity and show extreme genetic differentiation among each other and to field populations. Continued use of laboratory populations for initial soybean aphid resistance screening could lead to erroneous estimations of the potential success for host-plant resistance.

Keywords

soybean aphidAphis glycinesmicrosatellitesgenetic diversity
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 6 , December 2010 , pp. 727 - 734
Copyright
Copyright © Cambridge University Press 2010

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References

Arias, L., Bejarano, E.E., Márquez, E., Moncada, J., Vélez, I. & Uribe, S. (2005) Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae). Neotropical Entomology 34, 499–506.CrossRefGoogle Scholar
Arnaud-Haond, S. & Belkhir, K. (2007) Genclone: a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Molecular Ecology Notes 7, 15–17.CrossRefGoogle Scholar
Chen, CY., Gu, C., Mensah, C., Nelson, R.L. & Wang, D. (2007) SSR marker diversity of soybean aphid resistance sources in North America. Genome 50, 1104–1111.CrossRefGoogle ScholarPubMed
Clark, A.J. & Perry, K.L. (2002) Transmissibility of field isolates of soybean viruses by Aphis glycines. Plant Disease 86, 1219–1222.CrossRefGoogle ScholarPubMed
Coeur d'acier, A., Sembène, M., Audiot, P. & Rasplus, J.Y. (2004) Polymorphic microsatellite loci in the black aphid, Aphis fabae Scopoli, 1763 (Hemiptera, Aphididae). Molecular Ecology Notes 4, 306–308.CrossRefGoogle Scholar
Dieringer, D. & Schlotterer, C. (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Molecular Ecology Notes 3, 167–169.CrossRefGoogle Scholar
Dorken, M.E. & Eckert, C.G. (2001) Severely reduced sexual reproduction in northern populations of a clonal plant, Decodon verticillatus (Lythraceae). Journal of Ecology 89, 339–350.CrossRefGoogle Scholar
Fuller, S.J., Chavigny, P., Lapchin, L. & Vanlerberghe-Masutti, F. (1999) Variation in clonal diversity in glasshouse infestations of the aphid, Aphis gossypii Glover in southern France. Molecular Ecology 8, 1867–1877.CrossRefGoogle ScholarPubMed
Gauffre, B. & Coeur d'acier, A. (2006) New polymorphic microsatellite loci, cross-species amplification and PCR multiplexing in the black aphid, Aphis fabae Scopoli. Molecular Ecology Notes 6, 440–442.CrossRefGoogle Scholar
Goudet, J. (1995) FSTAT (Version 1.2): A computer program to calculate F-statistics. Journal of Heredity 86, 485–486.CrossRefGoogle Scholar
Goudet, J. (2001) FSTAT, A program to estimate and test gene diversities and fixation indices, Version 2.9.3. Available online at http://www2.unil.ch/popgen/softwares/fstat.htm (accessed 21 February 2010).Google Scholar
Haak, L., Simon, J.C., Gauthier, J.P., Plantegenest, M. & Dedryver, C.A. (2000) Evidence for predominant clones in a cyclically parthenogenetic organism provided by combined demographic and genetic analyses. Molecular Ecology 9, 2055–2066.CrossRefGoogle Scholar
Halkett, F., Plantegenest, M., Prunier-Leterme, N., Mieuzet, L., Delmotte, F. & Simon, J.C. (2005) Admixed sexual and facultatively asexual aphid lineages at mating sites. Molecular Ecology 14, 325–336.CrossRefGoogle ScholarPubMed
Hill, C.B., Li, Y. & Hartman, G.L. (2004) Resistance to soybean aphid in soybean germplasm. Crop Science 44, 98–106.CrossRefGoogle Scholar
Hill, C.B., Li, Y. & Hartman, G.L. (2006a) A single dominant gene for resistance to the soybean aphid in the soybean cultivar Dowling. Crop Science 46, 1601–1605.CrossRefGoogle Scholar
Hill, C.B., Li, Y. & Hartman, G.L. (2006b) Soybean aphid resistance in soybean Jackson is controlled by a single dominant gene. Crop Science 46, 1606–1608.CrossRefGoogle Scholar
Hodgson, D.J. (2001) Monoclonal aphid colonies and the measurement of clonal fitness. Ecological Entomology 26, 444–448.CrossRefGoogle Scholar
Iwaki, M., Roechan, M., Hibino, H., Tochihara, H. & Tantera, D.M. (1980) A persistent aphidborne virus of soybean, Indonesian soybean dwarf virus. Plant Disease 64, 1027–1030.CrossRefGoogle Scholar
Kanbe, T. & Akimoto, S.-I. (2009) Allelic diversity in long-term asexual populations of the pea aphid, Acrythosiphon pisum in comparison with sexual populations. Molecular Ecology 18, 801–816.CrossRefGoogle Scholar
Kim, K.S., French, B.W., Sumerford, D.V. & Sappington, T.W. (2007) Genetic diversity in laboratory colonies of western corn rootworm (Coleoptera: Chrysomelidae), including a nondiapause colony. Environmental Entomology 36, 637–645.CrossRefGoogle ScholarPubMed
Kim, K.S., Hill, C.B., Hartman, G.L., Mian, M.A.R. & Diers, B.W. (2008) Discovery of soybean aphid biotypes. Crop Science 48, 923–928.CrossRefGoogle Scholar
Lanzaro, G.C., Alexander, B., Mutebi, J.-P., Montoya-Lerma, J. & Warbug, A. (1998) Genetic variation among natural and laboratory colony populations of Lutzomyia longipalpis (Lutz and Neiva, 1912) (Diptera:Psychodidae) from Colombia. Memórias do Instituto Oswaldo Cruz 93, 65–69.CrossRefGoogle ScholarPubMed
Llewellyn, K.S., Loxdale, H.D., Harrington, R., Clark, S.J. & Sunnucks, P. (2004) Evidence for gene flow and local clonal selection in field populations of the grain aphid (Sitobion avenae) in Britain revealed using microsatellites. Heredity 93, 143–153.CrossRefGoogle ScholarPubMed
Loxdale, H.D. (2008) The nature and reality of the aphid clone: genetic variation, adaptation and evolution. Agricultural and Forest Entomology 10, 81–90.CrossRefGoogle Scholar
Loxdale, H.D. & Lushai, G. (2003) Rapid changes in clonal lineages: the death of a ‘sacred cow’. Biological Journal of the Linnean Society 79, 3–16.CrossRefGoogle Scholar
Macedo, T.B., Bastos, C.S., Higley, L.G., Ostlie, K.R. & Madhavan, S. (2003) Photosynthetic responses of soybean to soybean aphid (Homoptera: Aphididae) injury. Journal of Economic Entomology 96, 188–193.CrossRefGoogle ScholarPubMed
McCornack, B., Ragsdale, D.W. & Venette, R.C. (2004) Demography of soybean aphid (Homoptera: Aphididae) at summer temperatures. Journal of Economic Entomology 97, 854–861.CrossRefGoogle ScholarPubMed
Mian, M.A.R., Hammond, R.B. & St. Martin, S.K. (2008a) New plant introductions with resistance to the soybean aphid. Crop Science 48, 1055–1061.CrossRefGoogle Scholar
Mian, M.A.R., Kang, S.T., Beil, S.E. & Hammond, R.B. (2008b) Genetic linkage mapping of the soybean aphid resistance gene in PI 243540. Theoretical and Applied Genetics 117, 955–962.CrossRefGoogle Scholar
Michel, A., Zhang, W., Jung, J.K., Kang, S.T. & Mian, M.A.R. (2009a) Cross-species amplification and polymorphism of microsatellite loci in the soybean aphid, Aphis glycines. Journal of Economic Entomology 102, 1389–1392.CrossRefGoogle ScholarPubMed
Michel, A., Zhang, W., Jung, J.K., Kang, S.T. & Mian, M.A.R. (2009b) Population genetic structure of the soybean aphid, Aphis glycines. Environmental Entomology 38, 1301–1311.CrossRefGoogle Scholar
Müller, H.J. (1964) The relation of recombination to mutational advance. Mutation Research 1, 2–9.CrossRefGoogle Scholar
Nei, M. (1972) Genetic distance between populations. American Naturalist 106, 283–292.CrossRefGoogle Scholar
Nei, M. (1978) Estimation of heterozygosity and genetic distance from a small number of individuals. Genetics 76, 379–390.CrossRefGoogle Scholar
Peccoud, J., Figueroa, C.C., Silva, A.X., Ramirez, C.C., Mieuzet, L., Bonhomme, J., Stoeckel, S., Plantegenest, M. & Simon, J.C. (2008) Host range expansion of an introduced insect pest through multiple colonizations of specialized clones. Molecular Ecology 17, 4608–4618.CrossRefGoogle ScholarPubMed
Ragsdale, D.W., Voegtlin, D. & O'Neil, R.J. (2004) Soybean aphid biology in North America. Annals of the Entomological Society of America 97, 204–208.CrossRefGoogle Scholar
Ragsdale, D.W., McCornack, B.P., Venette, R.C., Potter, B.D., MacRae, I.V., Hodgson, E.W., O'Neal, M.E., Johnson, K.D., O'Neil, R.J., DiFonzo, C.D., Hunt, T.E., Glogoza, P.A. & Cullen, E.M. (2007) Economic threshold for the soybean aphid (Hemiptera: Aphididae). Journal of Economic Entomology 100, 1258–1267.CrossRefGoogle ScholarPubMed
Sunnucks, P., De Barro, P.J., Lushai, G., Maclean, N. & Hales, D.F. (1997) Genetic structure of an aphid studied using microsatellites: cyclic parthenogenesis, differentiated lineages and host specialization. Molecular Ecolology 6, 1059–1073.CrossRefGoogle ScholarPubMed
Van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M. & Shipley, P.F. (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4, 535–538.CrossRefGoogle Scholar
Vanlerberghe-Masutti, F., Chavigny, P. & Fuller, S. (1999) Characterization of microsatellite loci in the aphid species Aphis gossypii Glover. Molecular Ecology 8, 693–695.CrossRefGoogle ScholarPubMed
Vanoverbeke, J. & De Meester, L. (1997) Among-populational genetic differentiation in the cyclical parthenogen Daphnia magna (Crustacea, Anomopoda) and its relation to geographic distance and clonal diversity. Hydrobiologia 360, 135–142.CrossRefGoogle Scholar
Vennette, R.C. & Ragsdale, D.W. (2004) Assessing the invasion by soybean aphid (Homoptera: Aphididae): where will it end? Annals of the Entomological Society of America 97, 219–226.CrossRefGoogle Scholar
Vialette, A., Dedryver, C.A., Simon, J.C., Galman, M. & Plantegenest, M. (2005) Limited genetic exchanges between populations of an insect pest living on uncultivated and related cultivated host plants. Proceedings of the Royal Society, Series B 1567, 1075–1082.Google Scholar
Vorwerk, S. & Forneck, A. (2007) Analysis of genetic variation within clonal lineages of grape phylloxera (Daktulosphaira vitifoliae Fitch) using AFLP fingerprinting and DNA sequencing. Genome 50, 660–667.CrossRefGoogle ScholarPubMed
Wang, R.Y., Kritzman, A., Hershman, D.E. & Ghabrial, S.A. (2006) Aphis glycines as a vector of persistently and nonpersistently transmitted viruses and potential risks for soybean and other crops. Plant Disease 90, 920–926.CrossRefGoogle ScholarPubMed
Wang, X.B., Fang, Y.H., Lin, S.Z., Zhang, L.R. & Wang, H.D. (1994) A study on the damage and economic threshold of the soybean aphid at the seedling stage. Plant Protection 20, 12–13.Google Scholar
Zhang, G., Gu, C. & Wang, D. (2009) Molecular mapping of soybean aphid resistance genes in PI 567541B. Theoretical and Applied Genetics 118, 473–82.CrossRefGoogle ScholarPubMed