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Glossina swynnertoni (Diptera: Glossinidae): effective population size and breeding structure estimated by mitochondrial diversity

Published online by Cambridge University Press:  09 March 2007

J.G. Marquez ,
I.I. Malele ,
J.O. Ouma  and
E.S. Krafsur
J.G. Marquez
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
I.I. Malele
Affiliation:
Tsetse and Trypanosomiasis Research Institute, Tanga, Tanzania
J.O. Ouma
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
E.S. Krafsur*
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
*
*Fax: 515 294 5957 E-mail: ekrafsur@iastate.edu
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Abstract

Nucleotide diversity was examined at mitochondrial COI and r16S2 loci in eight Glossina swynnertoni Austen collections from northern Tanzania and from a culture maintained by the International Atomic Energy Agency. Eighteen composite haplotypes were observed among 149 flies, two of which were common to all samples and 10 were private. Mean haplotype diversity was 0.59 and nucleotide diversity was 0.0013. There were excess singular haplotypes and mutation-drift disequilibrium suggesting that populations had experienced an earlier bottleneck and subsequent expansion. Factorial correspondence analysis showed that haplotype frequencies varied much more temporally (GST = 0.18) than spatially (GST = 0.04). The estimate of effective population size Ne in Tarangire was a harmonic mean ~50 reproductive flies averaged over ~47 generations. The mean rate of gene flow was estimated to be ~5±1 reproducing females per generation but inflated because of mutation-drift disequilibrium arising from likely earlier bottlenecks.

Keywords

gene floweffective population sizemitochondrial variationtsetse
Type
Research Article
Information
Bulletin of Entomological Research , Volume 96 , Issue 4 , August 2006 , pp. 353 - 360
Copyright
Copyright © Cambridge University Press 2006

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