Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-10T17:20:01.333Z Has data issue: false hasContentIssue false
  • English
  • Français

The great spruce bark beetle (Dendroctonus micans Kug.) (Coleoptera: Scolytidae) in Lithuania: occurrence, phenology, morphology and communities of associated fungi

Published online by Cambridge University Press:  22 November 2016

A. Menkis ,
J. Lynikienė ,
A. Marčiulynas ,
A. Gedminas  and
A. Povilaitienė
A. Menkis*
Affiliation:
Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-75007 Uppsala, Sweden
J. Lynikienė
Affiliation:
Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, LT-53101 Kaunas District, Lithuania
A. Marčiulynas
Affiliation:
Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, LT-53101 Kaunas District, Lithuania
A. Gedminas
Affiliation:
Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, LT-53101 Kaunas District, Lithuania
A. Povilaitienė
Affiliation:
Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, LT-53101 Kaunas District, Lithuania
*
*Author for correspondence Phone: +46-18-672729 Fax: +46-18-673599 E-mail: audrius.menkis@slu.se
Get access Rights & Permissions [Opens in a new window]

Abstract

We studied the occurrence, morphology and phenology of Dendroctonus micans in Lithuania and the fungi associated with the beetle at different developmental stages. The occurrence of D. micans was assessed in 19 seed orchards (at least 40 years old) of Picea abies (L. Karst.) situated in different parts of the country. Bark beetle phenology was studied in two sites: a seed orchard of P. abies and a plantation of Picea pungens (Engelm.). D. micans morphology was assessed under the dissection microscope using individuals at different developmental stages that were sampled during phenology observations. Communities of fungi associated with D. micans were studied using both fungal culturing methods and direct high-throughput sequencing from D. micans. Results showed that the incidence D. micans was relatively rare and D. micans was mainly detected in central and eastern Lithuania. The life cycle included the following stages: adult, egg, I–V developmental stage larvae and pupa. However, development of D. micans was quicker and its nests larger under the bark of P. pungens than of P. abies, indicating the effect of the host species. Fungal culturing and direct high-throughput sequencing revealed that D. micans associated fungi communities were species rich and dominated by yeasts from a class Saccharomycetes. In total, 319 fungal taxa were sequenced, among which Peterozyma toletana (37.5% of all fungal sequences), Yamadazyma scolyti (30.0%) and Kuraishia capsulate (17.7%) were the most common. Plant pathogens and blue stain fungi were also detected suggesting their potentially negative effects to both tree health and timber quality.

Keywords

spruceclimate changefungisymbiosisforest pestsmicroorganismsbark beetle
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 4 , August 2017 , pp. 431 - 438
Check for updates
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alkan Akinci, H., Özcan, G.E. & Eroglu, M. (2003) Damage status of Dendroctonus micans (Kugelann) (Coleoptera: Scolytidae) and effectiveness of Rhizophagus grandis Gyllenhal (Coleoptera: Rhizophagidae) on it in the field. In XII World Forestry Congress, Qebec City, Canada. Available online at http://www.fao.org/docrep/ARTICLE/WFC/XII/0417-B3.HTM.Google Scholar
Atlas, R.M. (1997) Handbook of Microbiological Media. Boca Raton, Florida, CRC Press.Google Scholar
Bevan, D. & King, C.J. (1983) Dendroctonus micans Kug – a new pest of spruce in UK. Commonwealth Forestry Review 62, 41–51.Google Scholar
Boone, C.K., Six, D.L., Zheng, Y. & Raffa, K.F. (2008) Parasitoids and dipteran predators exploit volatiles from microbial symbionts to locate bark beetles. Environmental Entomology 37, 150–161.CrossRefGoogle ScholarPubMed
Castello, J.D., Shaw, C.G. & Furniss, M.M. (1976) Isolation of Cryptoporus volvatus and Fomes pinicola from Dendroctonus pseudotsugae . Phytopathology 66, 1431–1434.CrossRefGoogle Scholar
Chaves, L.F., Morrison, A.C., Kitron, U.D. & Scott, T.W. (2012) Nonlinear impacts of climatic variability on the density-dependent regulation of an insect vector of disease. Global Change Biology 18, 457–468.CrossRefGoogle Scholar
Christiansen, E. & Bakke, A. (1988) The spruce bark beetle of Eurasia. pp. 479–503 in Berryman, A.A. (Ed.) Dynamics of Forest Insects Populations. New York, Plenum Press.CrossRefGoogle Scholar
Clark, P.J. & Evans, F.C. (1954) Distance to nearest neighbor as a measure of spatial relationships in populations. Ecology 35, 445–453.CrossRefGoogle Scholar
Evans, H.F., King, C.J. & Wainhouse, D. (1984) Dendroctonus micans in the United Kingdom. The result of two years experience in survey and control. pp. 20–34 in Proceedings of the EEC Seminar on the Biological Control of Bark Beetles (Dendroctonus micans), Brussels.Google Scholar
Forestry Commision (2015) Great spruce bark beetle (Dendroctonus micans). Bristol, UK. Available online at http://www.forestry.gov.uk/greatsprucebeetle Google Scholar
Francke-Grosmann, H. & Ruhm, W. (1954) The control of Dendroctonus micans. Die Bekampfung des Riesenbastkafers (Dendroctonus micans Kug.). Zeitschrift fur Weltforstwirtschaft 17, 48–53.Google Scholar
Giordano, L., Garbelotto, M., Nicolotti, G. & Gonthier, P. (2012) Characterization of fungal communities associated with the bark beetle Ips typographus varies depending on detection method, location, and beetle population levels. Mycological Progress 12, 127–140.CrossRefGoogle Scholar
Ihrmark, K., Bodeker, I.T.M., Cruz-Martinez, K., Friberg, H., Kubartova, A., Schenck, J., Strid, Y., Stenlid, J., Brandstrom-Durling, M., Clemmensen, K.E. & Lindahl, B.D. (2012) New primers to amplify the fungal ITS2 region – evaluation by 454-sequencing of artificial and natural communities. Fems Microbiology Ecology 82, 666–677.CrossRefGoogle ScholarPubMed
Jacobs, K., Solheim, H., Wingfield, B.D. & Wingfield, M.J. (2005) Taxonomic re-evaluation of Leptographium lundbergii based on DNA sequence comparisons and morphology. Mycological Research 109, 1149–1161.CrossRefGoogle ScholarPubMed
Klavina, D., Menkis, A., Gaitnieks, T., Velmala, S., Lazdins, A., Rajala, T. & Pennanen, T. (2015) Analysis of Norway spruce dieback phenomenon in Latvia – a belowground perspective. Scandinavian Journal of Forest Research 31, 1–10.Google Scholar
Kurtzman, C., Fell, J.W. & Boekhout, T. (Eds) (2011) The Yeasts: A Taxonomic Study. Burlington, USA, Elsevier.Google Scholar
Lieutier, F., Vouland, G., Pettinetti, M., Garcia, J., Romary, P. & Yart, A. (1992) Defence reactions of Norway spruce (Picea abies Karst.) to artificial insertion of Dendroctonus micans Kug. (Col., Scolytidae). Journal of Applied Entomology 114, 174–186.CrossRefGoogle Scholar
Lim, Y.W., Kim, J.J., Lu, M. & Breuil, C. (2005) Determining fungal diversity on Dendroctonus ponderosae and Ips pini affecting lodgepole pine using cultural and molecular methods. Fungal Diversity 19, 79–94.Google Scholar
Linnakoski, R., de Beer, Z.W., Duong, T.A., Niemela, P., Pappinen, A. & Wingfield, M.J. (2012) Grosmannia and Leptographium spp. associated with conifer-infesting bark beetles in Finland and Russia, including Leptographium taigense sp nov. Journal of General and Molecular Microbiology 102, 375–399.Google ScholarPubMed
Lukasova, K., Holusa, J. & Knizek, M. (2014) Dendroctonus micans populations on Picea pungens in the center of a non-outbreak region contain few pathogens, parasites or predators: a new threat for urban forests? Urban Forestry and Urban Greening 13, 833–838.CrossRefGoogle Scholar
Magurran, A.E. (1988) Ecological Diversity and its Measurement. Princeton, NJ, USA, Princeton University Press.CrossRefGoogle Scholar
Menkis, A., Marčiulynas, A., Gedminas, A., Lynikienė, J. & Povilaitienė, A. (2015) High-throughput sequencing reveals drastic changes in fungal communities in the phyllosphere of Norway spruce (Picea abies) following invasion of the spruce bud scale (Physokermes piceae). Microbial Ecology 70, 904–911.CrossRefGoogle ScholarPubMed
Persson, Y., Vasaitis, R., Langstrom, B., Ohrn, P., Ihrmark, K. & Stenlid, J. (2009) Fungi vectored by the bark beetle Ips typographus following hibernation under the bark of standing trees and in the forest litter. Microbial Ecology 58, 651–659.CrossRefGoogle ScholarPubMed
Pettey, T.M. & Shaw, C.G. (1986). Isolation of Fomitopsis pinicola from inflight bark-beetles (Coleoptera: Scolytidae). Canadian Journal of Botany 64, 1507–1509.CrossRefGoogle Scholar
Sevim, A., Demir, I., Tanyeli, E. & Demirbag, Z. (2010) Screening of entomopathogenic fungi against the European spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae). Biocontrol Science and Technology 20, 3–11.CrossRefGoogle Scholar
Shannon, C.E. (1948) A mathematical theory of communication. Bell System Technical Journal 27, 379–423.CrossRefGoogle Scholar
Vega, F.E. & Blackwell, M. (2005) Insect-fungal Associations: Ecology and Evolution. Oxford, Oxford University Press.CrossRefGoogle Scholar
Voolma, K.K. (1980) The distribution and ecology of the European spruce beetle Dendroctonus micans Kug. (Coleoptera: Scolytidae) in Estonia. Metsanduslikud Uurimused 16, 44–51.Google Scholar
Wainhouse, D. & Beechgarwood, P. (1994) Growth and survival of Dendroctonus micans on 6 species of conifers. Journal of Applied Entomology 117, 393–399.CrossRefGoogle Scholar
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315–322 in Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds) PCR Protocols: A Guide to Methods and Applications. San Diego, USA: Academic Press, Inc.Google Scholar
Wicherham, L.J., Hansenula, H. & Sydow, P. (1970) The Yeasts - A Toxonomic Study. Amsterdam: North Holland Publishing Co.Google Scholar
Yilmaz, H., Sezen, K., Kati, H. & Demirbag, Z. (2006) The first study on the bacterial flora of the European spruce bark beetle, Dendroctonus micans (Coleoptera : Scolytidae). Biologia 61, 679–686.CrossRefGoogle Scholar
Supplementary material: File

Menkis supplementary material

Table S1

Download Menkis supplementary material(File)
File 69.9 KB