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Identification and metabolite profiling of Sitophilus oryzae L. by 1D and 2D NMR Spectroscopy

Published online by Cambridge University Press:  09 October 2009

A. Trivedi ,
P. Kaushik  and
A. Pandey
A. Trivedi*
Affiliation:
NMR Laboratory, Division of SAIF, Central Drug Research Institute, Chattar Manzil Palace, Post Box 173, Lucknow-226 001, India
P. Kaushik
Affiliation:
Institute of Pesticide Formulation and Technology, Sector-21, Gurgaon, Haryana-122016, India
A. Pandey
Affiliation:
Department of Zoology, Dayanand Anglo-Vedic College, Civil Lines, Kanpur-208001, (UP), India
*
*Author for correspondence Fax: +91-0512-2559280 E-mail: anky58@gmail.com
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Abstract

The polyphagous insect Sitophilus oryzae L. (Coleoptera:Curculionidae) has a tremendous adaptability in feeding behaviour, making it a serious invasive pest of stored cereals. The present study identifies the metabolite composition of Sitophilus oryzae (S. oryzae) using Nuclear Magnetic Resonance (NMR) spectroscopy. Assignment of 1D-proton by NMR, 1H-1H COSY, 2D-TOCSY 1H-1H, had been done. Amongst the various biochemically important metabolites isoleucine, valine, leucine, β-hydroxybutyrate, lysine, glutamate, glutamine, proline, lactate, alanine, di-methylamine, α-glucose, β-glucose, choline, glycerophosphorylcholine and tyrosine are present in S. oryzae. In wheat-fed S. oryzae, the presence of threonine and the absence of lactate is observed. In rice-fed S. oryzae, however, the presence of lactate and the absence of threonine were observed. Barley-fed S. oryzae shows presence of both tyrosine and lactate. It is concluded that the pest S. oryzae has adaptability on different stored cereals and grains, depicting the presence of earlier reported metabolites. The present study aims to identify the key metabolic components and associated enzymes in Sitophilus oryzae fed on different cereals.

Keywords

NMR spectroscopySitophilus oryzaestored grain pestmetabolites identificationpest control
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 3 , June 2010 , pp. 287 - 296
Copyright
Copyright © Cambridge University Press 2009

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References

Anonymous (1991) Législation et Réglementation Phytosanitaires au Maroc. DPVCTRF, Service de la Protection des Végetaux, pp. 112–121.Google Scholar
Baker, J.E. (1986) Amylase/proteinase ratios in larval midguts of ten stored-product insects. Entomologia Experimentalis et Applicata 40(1), 41–46.CrossRefGoogle Scholar
Baker, J.E. (1988) Dietary modulation of a-amylase activity in eight strains of Sitophilus oryzae and Sitophilus zeamais. Entomologia Experimentalis et Applicata 46, 47–52.CrossRefGoogle Scholar
Chapman, R.F. (1982) The Insects: Structure and Function. 3rd edn.919 pp. Cambridge, MA, USA, Harvard University Press.Google Scholar
Cohen, A.C. (2003) Insect Diets: Science and Technology. 429 pp. Boca Raton, FL, USA, CRC Press, LLC.CrossRefGoogle Scholar
Dowell, F.E., Throne, J.E., Wang, D. & Baker, J.E. (1999) Identifying stored-grain insects using near infra-red spectroscopy. Journal of Economic Entomology 92(1), 165–169.CrossRefGoogle Scholar
Fan, T.W.M. (1996) Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures. Progress in NMR spectroscopy 28, 161–219.CrossRefGoogle Scholar
Fields, G.P. & White, N.D.G. (2002) Alternatives to methyl bromide treatments for stored-product and quarantine insects. Annual Review of Entomology 47, 331–359.CrossRefGoogle ScholarPubMed
Gilmour, D. (1965) The Metabolism of Insects. 195 pp. Edinburgh, UK, Oliver and Boyd.Google Scholar
Govindan, S., Sundaram, S., Nagappan, R., Sundaram, J. & Savarimuthu, I. (2000) Efficacy of Beauveria bassiana for control of the rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Applied Entomology Zoology 36(1), 117–120.Google Scholar
Higham, D.P., Nicholson, J.K., Overnell, J. & Sadler, P.J. (1986) NMR studies of crab and plaice metallothioneins. Environmental Health Perspectives 65, 157–165.Google ScholarPubMed
Hill, D.S. (1990) Pests of Stored Products and Their Control. 274 pp. London, UK, Belhaven Press.Google Scholar
Liang, C., Brookhart, G., Feng, G.H., Reeck, G.R. & Kramer, K.J. (1991) Inhibition of digestive proteinases of stored grain Coleoptera by oryzacystatin, a cysteine proteinase inhibitor from rice seed. Federation of European Biochemical Societies 278, 139–142.CrossRefGoogle ScholarPubMed
Matthews, G.A. (1993) Insecticide application in stores. pp. 305–315in Matthews, G.A. & Hislop, E.C. (Eds) Application Technology for Crop Protection. Wallingford, UK, CABI International.CrossRefGoogle Scholar
Nicholson, J.K. & Wilson, I.D. (1989) High resolution proton NMR spectroscopy of biological fluids. Progress in Nuclear Magnetic Resonance Spectroscopy 21, 449–501.CrossRefGoogle Scholar
Paliwal, J., Wang, W., Symons, S.J. & Karunakaran, C. (2004) Insect species and infestation levels determination in stored wheat using near-infra red spectroscopy. Canadian Biosystems Engineering 46(7), 17–24.Google Scholar
Phalaraksh, C., Lenz, E.M., Lindon, J.K., Farrant, R.D., Reynolds, S.E., Wilson, I.D., Osborn, D. & Weeks, J.M. (1999) NMR spectroscopic studies on the haemolymph of the tobacco hornworm, Manduca sexta: assignment of 1H and 13C NMR spectra. Insect Biochemistry and Molecular Biology 29, 795–805.CrossRefGoogle Scholar
René, F. (1999) Insect P-450 enzymes. Annual Review of Entomology 44, 507–533.Google Scholar
Schwartz, B.E. & Burkholder, W.E. (1991) Development of the granary weevil (Coleoptera: Curculionidae) on barley, corn, oats, rice, and wheat. Journal of Economic Entomology 84, 1047–1052.CrossRefGoogle Scholar
Slavin, J. (2004) Whole grains and human health. Nutrition Research Reviews 17, 99–110.CrossRefGoogle ScholarPubMed
Terriere, L.C. (1984) Induction of detoxication enzymes in insects. Annual Review of Entomology 29, 71–88.CrossRefGoogle ScholarPubMed
Thompson, S.N. (1990) NMR spectroscopy: its basis, biological application and use in studies of insect metabolism. Insect Biochemistry 20(3), 223–237.CrossRefGoogle Scholar
Thompson, S.N. (1998) Long-term regulation of glucogenesis by dietary carbohydrate and relevance to blood sugar level in an insect Manduca sexta L. International Journal of Biochemistry and Cell Biology 30, 987–999.CrossRefGoogle Scholar
Thompson, S.N. & Borchardt, D.B. (1996) An in vivo 13C NMR study on the effect of dietary sugar on pyruvate cycling during glucogenesis from (2–13C) pyruvate in Manduca sexta L. Insect Biochemistry and Molecular Biology 26(10), 1047–1054.CrossRefGoogle Scholar
Wigglesworth, V.B. (1965) The Principles of Insect Physiology. 741 pp. London, UK, Methuen.Google Scholar