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Identification of a new P450s gene (AccCYP4AV1) and its roles in abiotic stress resistance in the Apis cerana cerana Fabricius

Published online by Cambridge University Press:  27 October 2020

Weixing Zhang Open the ORCID record for Weixing Zhang [Opens in a new window] ,
Hongfang Wang ,
Zhenguo Liu ,
Ying Wang  and
Baohua Xu
Weixing Zhang
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China
Hongfang Wang
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China
Zhenguo Liu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China
Ying Wang
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China
Baohua Xu*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China
*
Author for correspondence: Baohua Xu, Email: bhxu@sdau.edu.cn
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Abstract

Cytochrome P450 monooxygenases (P450s) play significant roles in protecting organisms from abiotic stress damage. Here, we report the sequence and characterization of a P450s gene (AccCYP4AV1), isolated from Apis cerana cerana Fabricius. The open reading frame of AccCYP4AV1 is 1506 base pairs long and encodes a predicted protein of 501 amino acids and 57.84 kDa, with an isoelectric point of 8.67. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis indicated that AccCYP4AV1 is more highly expressed in the midgut than in other tissues. In addition, the highest expression occurs in newly emerged adult workers, followed by the first instar of the larval stage. In addition, the expression of the AccCYP4AV1 was upregulated by low temperature (4 °C), ultraviolet radiation, hydrogen peroxide, paraquat, and dichlorvos treatments. In contrast, AccCYP4AV1 transcription was downregulated by other abiotic stress conditions: exposure to increased temperature (44 °C), deltamethrin, cadmium chloride, and mercury (II) chloride. Moreover, when AccCYP4AV1 was knocked-down by RNA interference, the results suggested that multiple antioxidant genes (AccsHSP22.6, AccSOD2, AccTpx1, and AccTpx4) were downregulated and antioxidant genes AccGSTO1 and AccTrx1 were upregulated. The activity levels of peroxidase and catalase were upregulated in the AccCYP4AV1-knocked-down samples, compared with those in the control groups. These findings suggest that the AccCYP4AV1 protein might be involved in the defense against abiotic stress damage.

Keywords

Abiotic stressAccCYP4AV1apis cerana cerana Fabriciuscytochrome P450 monooxygenasesRNA interference
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 1 , February 2021 , pp. 57 - 65
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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