Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-28T15:17:26.289Z Has data issue: false hasContentIssue false
  • English
  • Français

Investigation of insecticide-resistance status of Cydia pomonella in Chinese populations

Published online by Cambridge University Press:  17 March 2015

X.-Q. Yang  and
Y.-L. Zhang
X.-Q. Yang
Affiliation:
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling712100, Shaanxi, China Key Laboratory of Economical and Applied Entomology of Liaoning province, College of Plant Protection, Shenyang Agriculture University, Shenyang110866, Liaoning, China
Y.-L. Zhang*
Affiliation:
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling712100, Shaanxi, China
*
*Author for correspondence Phone: +86-29-8709-2190 Fax: +86-29-8709-2190 E-mail: yalinzh@nwsuaf.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The codling moth Cydia pomonella (L.) is an economically important fruit pest and it has been directly targeted by insecticides worldwide. Serious resistance to insecticides has been reported in many countries. As one of the most serious invasive pest, the codling moth has populated several areas in China. However, resistance to insecticides has not been reported in China. We investigated the insecticide-resistance status of four field populations from Northwestern China by applying bioassays, enzyme activities, and mutation detections. Diagnostic concentrations of lambda-cyhalothrin, chlorpyrifos-ethyl, carbaryl, and imidacloprid were determined and used in bioassays. Field populations were less susceptible to chlorpyrifos-ethyl and carbaryl than laboratory strain. Insensitive populations displayed an elevated glutathione S-transferases (GSTs) activity. Reduced carboxylesterase (CarE) activity was observed in some insecticide insensitive populations and reduced acetylcholinesterase activity was observed only in the Wuw population. The cytochrome P450 polysubstrate monooxygenases activities in four field populations were not found to be different from susceptible strains. Neither the known-resistance mutation F399V in the acetylcholinesterase (AChE) gene, ace1, nor mutations in CarE gene CpCE-1 were found in adult individuals from our field populations. Native-PAGE revealed that various CarE isozymes and AChE insensitivity were occurring among Chinese populations. Our results indicate that codling moth populations from Northwestern China were insensitivity to chlorpyrifos-ethyl and carbaryl. Increased GST activity was responsible for insecticides insensitivity. Decreased CarE activity, as well as the presence of CarE and AChE polymorphisms might also be involved in insecticides insensitivity. New management strategies for managing this pest are discussed.

Keywords

Cydia pomonellainsecticides resistancedetoxifying enzymesmanagementinvasions
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 3 , June 2015 , pp. 316 - 325
Check for updates
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abbott, W.S. (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.Google Scholar
Alon, M., Alon, F., Nauen, R. & Morin, S. (2008) Organophosphates resistance in the B-biotype of Bemisia tabaci (Hemiptera: Aleyrodidae) is associated with a point mutation in an ace1-type acetylcholinesterase and overexpression of carboxylesterase. Insect Biochemistry and Molecular Biology 38, 940–949.CrossRefGoogle Scholar
Baek, J.H., Kim, J.I., Lee, D.W., Chung, B.K., Miyata, T. & Lee, S.H. (2005) Identification and characterization of ace1-type acetylcholine sterase likely associated with organophosphate resistance in Plutella xylostella . Pesticide Biochemistry and Physiology 81, 164–175.Google Scholar
Bouvier, J.C., Brosse, V. & Sauphanor, B. (1995) La résistance du carpocapse. L' Arboriculture Fruitière 479, 21–23.Google Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Brun-Barale, A., Bouvie, J.C., Pauron, D., Bergé, J.B. & Sauphanor, B. (2005) Involvement of a sodium channel mutation in pyrethroid resistance in Cydia pomonella L. and development of a diagnostic test. Pest Management Science 61, 549–554.CrossRefGoogle ScholarPubMed
Brunner, J.F., Beers, E.H., Dunley, J.E., Doerr, M. & Granger, K. (2005) Role of neonicotinyl insecticides in Washington apple integrated pest management. Part I. Control of lepidopteran pests. Journal of Insect Science 5, 14–0.CrossRefGoogle ScholarPubMed
Bush, M.R., Abdel-All, Y.A.I. & Rock, G.C. (1993) Parathion resistance and esterase activity in codling moth (Lepidoptera: Tortricidae) from North Carolina. Journal of Economic Entomology 86, 660–666.Google Scholar
Cassanelli, S., Reyes, M., Rault, M., Manicardi, G.C. & Sauphanor, B. (2006) Acetylcholinesterase mutation in an insecticide-resistant population of the codling moth Cydia pomonella (L.). Insect Biochemistry and Molecular Biology 36, 642–653.Google Scholar
Dunley, J.E. & Welter, S.C. (2000) Correlated insecticide cross-resistance in azinphosmethyl resistant codling moth (Lepidoptera: Tortricidae). Journal of Economic Entomology 93, 955–962.Google Scholar
Fuentes-Contreras, E., Reyes, M., Barros, W. & Sauphanor, B. (2007) Evaluation of azinphos-methyl resistance and activity of detoxifying enzymes in codling moth (Lepidoptera: Tortricidae) from central Chile. Journal of Economic Entomology 100, 551–556.Google Scholar
Giliomee, J.H. & Riedl, H. (1998) A century of codling moth control in South Africa. I. Historical perspective. Journal of the Southern African Society for Horticultural Sciences (South Africa) 8, 27–31.Google Scholar
Hollomon, D. (2012) Do we have the tools to manage resistance in the future? Pest Management Science 68, 149–154.CrossRefGoogle ScholarPubMed
Kim, Y.H. & Lee, S.H. (2013) Which acetylcholinesterase functions as the main catalytic enzyme in the Class Insecta? Insect Biochemistry and Molecular Biology 43, 47–53.CrossRefGoogle ScholarPubMed
Landolt, P.J., Suckling, D.M. & Judd, G.J.R. (2007) Positive interaction of a feeding attractant and a host kairomone for trapping the codling moth, Cydia pomonella (L.). Journal of Chemical Ecology 33, 2236–2244.Google Scholar
Men, Q.L., Chen, M.H., Zhang, Y.L. & Feng, J.N. (2013) Genetic structure and diversity of a newly invasive species, the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) in China. Biological Invasions 15, 447–458.Google Scholar
Newcomb, R.D., Campbell, P.M., Ollis, D.L., Cheah, E., Russell, R.J. & Oakeshott, J.G. (1997) A single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly. Proceedings of the National Academy of Science of the United States of America 94, 7464–7468.Google Scholar
Reuveny, H. & Cohen, E. (2004) Resistance of the codling moth Cydia pomonella (L.) (Lep., Tortricidae) to pesticides in Israel. Journal of Economic Entomology 128, 645–651.Google Scholar
Reyes, M. & Sauphanor, B. (2008) Resistance monitoring in codling moth: a need for standardization. Pest Management Science 64, 945–953.Google Scholar
Reyes, M., Bouvier, J.C., Bouvin, T., Fuentes-Contreras, E. & Sauphanor, B. (2004) Susceptibilidad a insecticidas y actividad enzimática de Cydia pomonella L. (Lepidop-tera: Tortricidae) provenientes de tres huertos de manzano de la región del Maule. Chile Agric Técn (Chile) 64, 229–237.Google Scholar
Reyes, M., Frank, P., Charmillot, P.J., Ioriatti, C., Olivares, J., Pasqualini, E. & Sauphanor, B. (2007) Diversity of insecticide resistance mechanisms and spectrum in European populations of codling moth, Cydia pomonella . Pest Management Science 63, 890–902.CrossRefGoogle ScholarPubMed
Reyes, M., Collange, B., Rault, M., Casanelli, S. & Sauphanor, B. (2011) Combined detoxification mechanisms and target mutation fail to confer a high level of resistance to organophosphates in Cydia pomonella (L.) (Lepidoptera: Tortricidae). Pesticide Biochemistry and Physiology 99, 25–32.CrossRefGoogle Scholar
Rodríguez, M.A., Bosch, D. & Sauphanor, B. (2010) Avilla J, Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae). Journal of Economic Entomology 103, 482–491.CrossRefGoogle ScholarPubMed
Rodríguez, M.A., Bosch, D. & Avilla, J. (2011 a) Resistance of Spanish codling moth (Cydia pomonella) populations to insecticides and activity of detoxifying enzymatic systems. Entomologia Experimentalis et Applicata 138, 184–192.Google Scholar
Rodríguez, M.A., Marques, T., Bosch, D. & Avilla, J. (2011 b) Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae). Pesticide Biochemistry and Physiology 100, 151–159.Google Scholar
Sauphanor, B., Bouvier, J. & Brosse, V. (1998) Spectrum of insecticide resistance in Cydia pomonella (Lepidoptera: Tortricidae) in Southeastern Franc. Journal of Economic Entomology 91, 1225–1231.Google Scholar
Thwaite, W.G., Williams, D.G. & Hately, A.M. (1993) Extent and significance of azinphos-methyl resistance in codling moth in Australia. pp. 166–168 in Corey, S., Dall, D. & Milne, W. (Eds) Pest Control and Sustainable Agriculture. Melbourne, Australia, Commonwealth Scientific and Industrial Research Organization.Google Scholar
Varela, L.G., Welter, S.C., Jones, V.P., Brunner, J.F. & Riedl, H. (1993) Monitoring and characterization of insecticide resistance in codling moth (Lepidoptera, Tortricidae) in four western states. Journal of Economic Entomology 86, 1–10.Google Scholar
Voudouris, C.C., Sauphanor, B., Frank, P., Reyes, M., Mamuris, Z., Tsitsipis, J.A., Vontas, J. & Margaritopoulos, J.T. (2011) Insecticide resistance status of the codling moth Cydia pomonella (Lepidoptera: Tortricidae) from Greece. Pesticide Biochemistry and Physiology 100, 229–238.CrossRefGoogle Scholar
Waldner, W. (1993) Rückblick und Vorschau auf die Bekämpfung des Apfelwicklers. Obstbau-Weinbau 12, 355–357.Google Scholar
Wheelock, C.E., Phillips, B.M., Anderson, B.S., Miller, J.L. & Hammock, B.D. (2008) Applications of carboxylesterase activity in environmental monitoring and toxicity identification evaluations (TIEs). pp. 117–178 in Whitacre, D.M. (ed.) Reviews of Environmental Contamination and Toxicology, New York, Springer.Google Scholar
Yang, X.Q. & Zhang, Y.L. (2013) Effect of temperature and sorbitol in improving the solubility of carboxylesterases protein CpCE-1 from Cydia pomonella and biochemical characterization. Applied Microbiology and Biotechnology 97, 10423–10433.Google Scholar
Yang, X.Q., Li, X.C. & Zhang, Y.L. (2013) Molecular cloning and expression of CYP9A61: a chlorpyrifos-ethyl and lambda-cyhalothrin-inducible cytochrome P450 cDNA from Cydia pomonella . International Journal of Molecular Science 14, 24211–24229.Google Scholar
Yang, X.Q., Liu, J.Y., Li, X.C., Chen, M.H. & Zhang, Y.L. (2014) Key amino acid associated with acephate detoxification by Cydia pomonella carboxylesterase based on molecular dynamics with alanine scanning and site-directed mutagenesis. Journal of Chemical Information and Modeling 54, 1350–1370.Google Scholar
Yang, Y., Wu, Y., Chen, S., Devine, G.J., Denholm, I., Jewess, P. & Moores, G.D. (2004) The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia. Insect Biochemistry and Molecular Biology 34, 763–773.CrossRefGoogle ScholarPubMed
Zhang, X.Z. (1957) Taxonomic notes on the codling moth, Carpocapsa pomonella L. in Sinkiang. Acta Entomologica Sinica 7, 467–472.Google Scholar
Zhao, P., Wang, Y. & Jiang, H. (2013) Biochemical properties, expression profiles, and tissue localization of orthologous acetylcholinesterase-2 in the mosquito, Anopheles gambiae . Insect Biochemistry and Molecular Biology 43, 260–271.Google Scholar