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Large-scale rearing of tsetse flies (Glossina spp.) in the absence of a living host

Published online by Cambridge University Press:  10 July 2009

A. R. Mews ,
P. A. Langley ,
R. W. Pimley  and
M. E. T. FLOOD
A. R. Mews
Affiliation:
Tsetse Research Laboratory, University of Bristol School of Veterinary Science, Langford, Bristol, BS18 7DU, U.K.
P. A. Langley
Affiliation:
Tsetse Research Laboratory, University of Bristol School of Veterinary Science, Langford, Bristol, BS18 7DU, U.K.
R. W. Pimley
Affiliation:
Tsetse Research Laboratory, University of Bristol School of Veterinary Science, Langford, Bristol, BS18 7DU, U.K.
M. E. T. FLOOD
Affiliation:
Tsetse Research Laboratory, University of Bristol School of Veterinary Science, Langford, Bristol, BS18 7DU, U.K.
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Abstract

A successful technique for feeding colonies of Glossina morsitans morsitans Westw. and G. austeni Newst. in the absence of living hosts is described. Insects are fed through membranes made of silicone rubber or of agar and Parafilm, overlying blood pools poured onto grooved glass plates. The diet employed is fresh pig blood, collected at slaughter, and aseptic procedures are adopted at every stage of diet preparation and presentation. The reproductive performance of these in vitro-fed colonies in terms of adult survival, fecundity, and offspring size, is the same as that of colonies fed on living hosts, when compared over a long period of time. The fact that the technique is successful when used with a diet of pig blood, but is not successful when used with cow blood prepared in the same way, suggests that the technique per se is adequate to elicit a normal feeding response from these tsetse species. The nature of the nutritional superiority of pig blood is not understood.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 67 , Issue 1 , March 1977 , pp. 119 - 128
Copyright
Copyright © Cambridge University Press 1977

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References

Azevedo, J. F.. DE PinhÃO., R.., Santos, A. M. T.. Dos, A. E., Ferreira, A. E.. (1968). Studies carried out with the Glossina morsitans colony of Lisbon. I. Some aspects of the evolution of the Glossina morsitans colony of Lisbon.—;An. Esc. nac. Saúde pública e Med. trop. 2, 19–42.Google ScholarPubMed
Bauer, B.. (1974). Bacterial infections in Glossina morsitans Westwood fed through membranes.—Proc. 3rd Int. Congr. Parasitol., München 1974, 2, 943.Google Scholar
Bauer, B.. & Wetzel, H.. (1976). A new membrane for feeding Glossina morsitans Westw. (Diptera, Glossinidae).—Bull. ent. Res. 65, 563–565.CrossRefGoogle Scholar
Curtis, C. F.. & Jordan, A. M.. (1970). Calculations of the productivity of Glossina austeni Newst. maintained on goats and on lop-eared rabbits.—Bull. ent. Res. 59, 651–658.CrossRefGoogle ScholarPubMed
Dethier, V. G.. (1954). Notes on the biting response of tsetse flies.—Am. J. trop. Med. Hyg. 3, 160–171.Google Scholar
Galun, R.. & Rice, M. J.. (1971). Role of blood platelets in haematophagy.—Nature New Biol. 233, 110–111.CrossRefGoogle ScholarPubMed
Gatehouse, A. G.. (1970). The probing response of Stomoxys calcitrans to certain physical and olfactory stimuli.—J. Insect Physiol. 16, 61–74.CrossRefGoogle ScholarPubMed
Itard, J.. & Maillot, L.. (1970). Les élevages de Glossines à Maisons-Alfort (France).—In Tsetse fly breeding under laboratory conditions and its practical application. 1st International Symposium, 22 & 23 April 1969. 125–136. Lisbon, Junta de Investigaçōes do Ultramar.Google Scholar
Jordan, A. M.. & Curtis, C. F.. (1968). Productivity of Glossina austeni Newst. maintained on lop-eared rabbits.—Bull. ent. Res. 58, 399–410.Google Scholar
Jordan, A. M.. & Curtis, C. F.. (1972). Productivity of Glossina morsitans morsitans West-wood maintained in the laboratory, with particular reference to the sterile-insect release method.—Bull. Wld Hlth Org. 46, 33–38.Google Scholar
Langley, P. A.. (1972). The role of physical and chemical stimuli in the development of in vitro feeding techniques for tsetse flies Glossina spp. (Dipt., Glossinidae).—Bull. ent. Res. 62, 215–228.CrossRefGoogle Scholar
Langley, P. A.. (1976). Initiation and regulation of ingestion by haematophagous arthropods. A review.—J. Med. Ent. 13, 121–130.CrossRefGoogle Scholar
Langley, P. A.. & Pimley, R. W.. (1975). Quantitative aspects of reproduction and larval nutrition in Glossina morsitans morsitans Westw. (Diptera, Glossinidae) fed in vitro.—Bull. ent. Res. 65, 129–142.CrossRefGoogle Scholar
Mews, A. R.., Baumgartner, H.., Luger, D.. & Offori, E. D.. (1976). Colonisation of Glossina morsitans morsitans Westw. (Diptera, Glossinidae) in the laboratory using in vitro feeding techniques.—Bull. ent. Res. 65, 631–642.CrossRefGoogle Scholar
Mews, A. R.., Offori, E.., Baumgartner, H.. & Luger, D.. (1972). Techniques used at the IAEA laboratory for rearing the tsetse fly Glossina morsitans Westw. In ISCTR Thirteenth meeting, Lagos, 1971. 243–254.Google Scholar
Mews, A. R.. & Ruhm, F.. (1971). Maintenance of Glossina morsitans Westwood fed through an artificial membrane on defibrinated blood. In Sterility principle for insect control or eradication. Proc. IAEA/FAO Symp., Athens, 14–18 September 1970, 283–293. Vienna, IAEA. (Proc. ser. ST1/PUB/265).Google Scholar
Mitchell, B. K.. (1976). ATP reception by the tsetse fly, Glossina morsitans Westw.—Experientia 32, 192–194.Google Scholar
Nash, T. A. M.., Jordan, A. M.. & Boyle, J. A.. (1968). The large-scale rearing of Glossina austeni Newst. in the laboratory IV.—The final technique.—Ann. trop. Med. Parasitol. 62, 336–341.Google Scholar
Nash, T. A. M.., Jordan, A. M.. & Trewern, M. A.. (1971). Mass rearing of tsetse flies (Glossina spp.): recent advances. In Sterility principle for insect control or eradication. Proc. IAEA/FAO Symp., Athens, 14–18 September 1970, 99–110. Vienna IAEA. (Proc. ser. ST1/PUB/265).Google Scholar
Vale, G. A.., Hargrove, J. W.., Jordan, A. M.., Langley, P. A.. & Mews, A. R.. (1976). Survival and behaviour of tsetse flies (Diptera, Glossinidae) released in the field: a comparison between wild flies and animal-fed and in vitro-fed laboratory reared flies.—Bull. ent. Res. 66, 731–744.CrossRefGoogle Scholar