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The maintenance of laboratory colonies of Culicoides variipennis (Coq.), C. nubeculosus (Mg.) and C. riethi Kieff. (Diptera, Ceratopogonidae)

Published online by Cambridge University Press:  10 July 2009

J. Boorman
J. Boorman
Affiliation:
Animal Virus Research Institute, Pirbright, Working, Surrey GU24 0NF, England
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Abstract

A method is described by which Culicoides variipennis (Coq.), C. riethi Kieff. and C. nubeculosus (Mg.) have been maintained as laboratory colonies for over five years. Techniques are given for the maintenance of adults, larvae and pupae; with some modifications these could probably be applied to a range of other species. Adult midges were held in waxed card pill boxes, and provided with sugar solution. Anaesthetised mice provided a blood-meal source. Larvae were reared in pans on a substrate of glass fibre in a medium which contained nutrient broth, grass meal and Bemax. The life-cycle of these species occupies 10–21 days at 23–25°C.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 64 , Issue 3 , November 1974 , pp. 371 - 377
Copyright
Copyright © Cambridge University Press 1974

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