Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T23:11:26.192Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France

Published online by Cambridge University Press:  27 May 2010

E. Blanchet ,
L. Blondin ,
P.A. Gagnaire ,
A. Foucart ,
J.M. Vassal  and
M. Lecoq
E. Blanchet*
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
L. Blondin
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
P.A. Gagnaire
Affiliation:
Institut des Sciences de l'Evolution, UMR 5554, Université Montpellier 2, cc065, place E. Bataillon, 34095Montpellier cedex 05, France
A. Foucart
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
J.M. Vassal
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
M. Lecoq
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
*
*Author for correspondence Fax: +33 (0)4 67 59 38 73 E-mail: blanchet.elodie@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C. wattenwylianus, C. siciliae and C. barbarus, in southern regions of France.

Keywords

Calliptamusspecies identificationmolecular diagnosticsmitochondrial Cytochrome Oxydase I
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 6 , December 2010 , pp. 701 - 706
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Behura, S.K. (2006) Molecular marker systems in insects: current trends and future avenues. Molecular Ecology 15, 3087–3113.CrossRefGoogle ScholarPubMed
Bensasson, D., Zhang, D.X. & Hewitt, G.M. (2000) Frequent assimilation of mitochondrial DNA by grasshopper nuclear genomes. Molecular Biology and Evolution 17, 406–415.CrossRefGoogle ScholarPubMed
Blaxter, M.L. (2004) The promise of a DNA taxonomy. Philosophical Transactions of the Royal Society of London, Series B: Biological Sciences 359, 669–679.CrossRefGoogle ScholarPubMed
Chopard, L. (1943) Orthoptéroïdes de l'Afrique du Nord. 450 pp. Paris, France, Larose.Google Scholar
Chopard, L. (1951) Faune de France n°56: Orthoptéroïdes. 359 pp. Paris, France, Paul Lechevalier.Google Scholar
Contreras-Diaz, H.G., Lopez, H., Oromi, P. & Juan, C. (2006) Microsatellite loci development in endangered pamphagid grasshoppers endemic to the Canary Islands (Orthoptera). Conservation Genetics 7, 767–771.CrossRefGoogle Scholar
COPR (1982) The Locust and Grasshopper Agricultural Manual. 690 pp. London, UK, Centre for Overseas Pest Research.Google Scholar
Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19, 11–15.Google Scholar
Fenn, J.D., Cameron, S.L. & Whiting, M.F. (2007) The complete mitochondrial genome sequence of the Mormon cricket (Anabrus simplex: Tettigoniidae: Orthoptera) and an analysis of control region variability. Insect Molecular Biology 16, 239–252.CrossRefGoogle Scholar
Fontana, P., Buzzeti, F.M., Cogo, A. & Odé, B. (2002) Guida al Riconoscimento e allo Studio di Cavallette, Grilli, Mantidi e Insetti Affini del Veneto. 592 pp. Vicence, Italia, Museo Naturalistico Archeologico.Google Scholar
Harz, K. (1975) Die Orthopteren Europas. The Orthoptera of Europe. 939 pp. Den Haag, The Netherlands, Dr W. Junk NV.CrossRefGoogle Scholar
Hinomoto, N., Muraji, M., Noda, T., Shimizu, T. & Kawasaki, K. (2004) Identification of five Orius species in Japan by multiplex polymerase chain reaction. Biological Control 31, 276–279.CrossRefGoogle Scholar
Hosseini, R., Keller, M.A., Schmidt, O. & Framenau, V.W. (2007) Molecular identification of wolf spiders (Araneae: Lycosidae) by multiplex polymerase chain reaction. Biological Control 40, 128–135.CrossRefGoogle Scholar
Jago, N.D. (1963) A revision of the genus Calliptamus Serville (Orthoptera: Acrididae). Bulletin of the British Museum, Natural history (Entomology) 13, 290–350.Google Scholar
Nolan, D.V., Carpenter, S., Barber, J., Mellor, P.S., Dallas, J.F., Mordue, A.J. & Piertney, S.B. (2007) Rapid diagnostic PCR assays for members of the Culicoides obsoletus and Culicoides pulicaris species complexes, implicated vectors of bluetongue virus in Europe. Veterinary Microbiology 124, 82–94.CrossRefGoogle ScholarPubMed
Olmo Vidal, J.M. (2006) Atlas dels Ortòpters de Catalunya i llibre vermell. 428 pp. Barcelona, Spain, Generalitat de Catalunya.Google Scholar
Phuc, H.K., Ball, A.J., Son, L., Hanh, N.V., Tu, N.D., Lien, N.G., Verardi, A. & Townson, H. (2003) Multiplex PCR assay for malaria vector Anopheles minimus and four related species in the Myzomyia Series from Southeast Asia. Medical and Veterinary Entomology 17, 423–428.CrossRefGoogle ScholarPubMed
Saccaggi, D.L., Kruger, K. & Pietersen, G. (2008) A multiplex PCR assay for the simultaneous identification of three mealybug species (Hemiptera: Pseudococcidae). Bulletin of Entomological Research 98, 27–33.CrossRefGoogle ScholarPubMed
Song, H., Buhay, J.E., Whiting, M.F. & Crandall, K.A. (2008) Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proceedings of the National Academy of Sciences of the United States of America 105, 13486–13491.CrossRefGoogle ScholarPubMed
Ståhls, G., Vujic, A., Pérez-Bañon, C., Radenkovic, S., Rojo, S. & Petanidous, T. (2009) COI barcodes for Identification of Merodon hoverflies (Diptera, Syrphidae) of Lesvos Island, Greece. Molecular Ecology Resources 9, 1431–1438.CrossRefGoogle ScholarPubMed
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.CrossRefGoogle ScholarPubMed