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Population structure of the banana weevil, an introduced pest in the Canary Islands, studied by RAPD analysis

Published online by Cambridge University Press:  12 November 2007

C. Magaña ,
B. Beroiz ,
P. Hernández-Crespo ,
M. Montes de Oca ,
A. Carnero ,
F. Ortego  and
P. Castañera
C. Magaña
Affiliation:
Dpto. de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC. Madrid, Spain
B. Beroiz
Affiliation:
Dpto. de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC. Madrid, Spain
P. Hernández-Crespo
Affiliation:
Dpto. de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC. Madrid, Spain
M. Montes de Oca
Affiliation:
Escuela Técnica Superior de Ingenieria Agraria, U.L.L. La Laguna-Tenerife, Spain
A. Carnero
Affiliation:
Dpto. Protección Vegetal del I.C.I.A., Apdo. 60, 38200 La Laguna-Tenerife, Spain
F. Ortego
Affiliation:
Dpto. de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC. Madrid, Spain
P. Castañera*
Affiliation:
Dpto. de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC. Madrid, Spain
*
*Author for correspondence Fax: +34 91 536 0432 E-mail: castan@cib.csic.es
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Abstract

The banana weevil (BW), Cosmopolites sordidus (Coleoptera: Curculionidae), is one of the most important insect pests of bananas and plantains. The mobility and the origin of BW infestations at the Canary Islands (Tenerife, La Gomera and La Palma) have been analysed using Random Amplified Polymorphic DNA (RAPD) as molecular markers. Populations from Costa Rica, Colombia, Uganda and Madeira were also included for comparison. One hundred and fifteen reproducible bands from eight primers were obtained. The level of polymorphism in the populations from the Canary Islands (40–62%) was in the range of those found in other populations. Nei's genetic distances, pair-wise fixation index (FST) values indicate that the closest populations are Tenerife populations among themselves (Nei's genetic distance=0.054–0.100; FST=0.091–0.157) and Costa Rica and Colombia populations (Nei's genetic distance=0.049; FST=0.113). Our results indicate the existence of BW local biotypes with limited gene flow and affected by genetic drift. These results are compatible with a unique event of colonization at Tenerife; whereas, the outbreaks in La Gomera and La Palma may come from independent introductions. The Madeira population is phylogenetically and geographically closer to the Canary Islands populations, suggesting that it is the most likely source of the insects introduced in the Canary Islands.

Keywords

molecular markersCosmopolites sordidusgenetic structuregene flow
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 6 , December 2007 , pp. 585 - 590
Copyright
Copyright © Cambridge University Press 2007

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