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A simple molecular identification method of the Thrips tabaci (Thysanoptera: Thripidae) cryptic species complex

Published online by Cambridge University Press:  09 December 2019

Péter Farkas ,
Zsuzsanna György ,
Annamária Tóth ,
Annamária Sojnóczki  and
József Fail Open the ORCID record for József Fail [Opens in a new window]
Péter Farkas
Affiliation:
Department of Entomology, Faculty of Horticultural Science, Szent István University, Budapest, Hungary
Zsuzsanna György
Affiliation:
Department of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Budapest, Hungary
Annamária Tóth
Affiliation:
Department of Plant Pathology, Faculty of Horticultural Science, Szent István University, Budapest, Hungary
Annamária Sojnóczki
Affiliation:
Department of Entomology, Faculty of Horticultural Science, Szent István University, Budapest, Hungary
József Fail*
Affiliation:
Department of Entomology, Faculty of Horticultural Science, Szent István University, Budapest, Hungary
*
Author for correspondence: József Fail, Email: fail.jozsef@kertk.szie.hu
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Abstract

The onion thrips (Thrips tabaci Lindeman, 1889) is a key pest of a wide range of crops because of its ecological attributes such as polyphagy, high reproduction rate, ability to transmit tospoviruses and resistance to insecticides. Recent studies revealed that T. tabaci is a cryptic species complex and it has three lineages (leek-associated arrhenotokous L1-biotype, leek-associated thelytokous L2-biotype and tobacco-associated arrhenotokous T-biotype), however, the adults remain indistinguishable. T. tabaci individuals were collected from different locations of Hungary to create laboratory colonies from each biotypes. Mitochondrial COI (mtCOI) region was sequenced from morphologically identified individuals. After sequence analysis SNPs were identified and used for CAPS marker development, which were suitable for distinguishing the three T. tabaci lineages. Genetic analysis of the T. tabaci species complex based on mtCOI gene confirmed the three well-known biotypes (L1, L2, T) and a new biotype because the new molecular evidence presented in this study suggests T-biotype of T. tabaci forming two distinct (sub)clades (T1 and T2). This genetic finding indicates that the genetic variability of T. tabaci populations is still not fully mapped. We validated our developed marker on thrips individuals from our thrips colonies. The results demonstrated that the new marker effectively identifies the different T. tabaci biotypes. We believe that our reliable genotyping method will be useful in further studies focusing on T. tabaci biotypes and in pest management by scanning the composition of sympatric T. tabaci populations.

Keywords

CAPS markerCOIcryptic species complexidentificationmitochondrial DNAgenetic analysisThrips tabaci
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 3 , June 2020 , pp. 397 - 405
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Copyright
Copyright © Cambridge University Press 2019

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