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Two novel cytochrome P450 genes CYP6CS1 and CYP6CW1 from Nilaparvata lugens (Hemiptera: Delphacidae): cDNA cloning and induction by host resistant rice

Published online by Cambridge University Press:  08 July 2010

Z. Yang ,
Y. Zhang ,
X. Liu  and
X. Wang
Z. Yang*
Affiliation:
College of Life Sciences, Hubei University, Wuhan 430062, China
Y. Zhang
Affiliation:
College of Life Sciences, Hubei University, Wuhan 430062, China
X. Liu
Affiliation:
College of Life Sciences, Hubei University, Wuhan 430062, China
X. Wang
Affiliation:
College of Life Sciences, Hubei University, Wuhan 430062, China
*
*Author for correspondence Fax: +86-27-87214327 E-mail: sailyangzhf@yahoo.com.cn
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Abstract

Two novel full-length P450 cDNAs, CYP6CS1 and CYP6CW1, were cloned from the fourth instar nymphs of brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) reared on its susceptible rice variety Taichung Native 1 (TN1) plants. The deduced proteins are typical microsomal P450s sharing conserved structural and functional domains with other insect CYP6 members. Temporal expression analysis by northern blot hybridization indicated pre-exposure to N. lugens moderately resistant rice Minghui 63 (MH63) seedlings caused a time course-dependent induction of CYP6CS1 which peaked after 24 h of treatment; in contrast, CYP6CW1 was induced and remained at a constant time course from 0–72 h. CYP6CS1 and CYP6CW1 are dramatically induced in gut tissues and, slightly upregulated in carcass and fat bodies as revealed in spatial gene expression analysis. Whole mount in situ hybridizaion revealed that the two genes are expressed at a basal level in gut tissue and Malpighian tubules in nymphs fed with TN1 rice. After exposure to MH63, the expression of CYP6CW1 was found to be high in the whole gut, including Malpighian tubules. Expression of CYP6CS1 was significantly increased in midgut, and slightly increased in foregut, hindgut and Malpighian tubules. These data suggest a potential role of the two P450s in determining patterns of N. lugens-rice relationships through allelochemical detoxification.

Keywords

brown planthopperNilaparvata lugens (Hemiptera: Delphacidae)cytochrome P450 monooxygenasehost rice resistanceallelochemical detoxification
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 1 , February 2011 , pp. 73 - 80
Copyright
Copyright © Cambridge University Press 2010

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