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ALIMENTARY CANAL OF ADULT ACALYMMA VITTATA (COLEOPTERA: CHRYSOMELIDAE): MORPHOLOGY AND POTENTIAL ROLE IN SURVIVAL OF ERWINIA TRACHEIPHILA (ENTEROBACTERIACEAE)

Published online by Cambridge University Press:  31 May 2012

Carlos Garcia-Salazar ,
F.E. Gildow ,
S.J. Fleischer ,
D. Cox-Foster  and
F.L. Lukezic
Carlos Garcia-Salazar
Affiliation:
Department of Entomology, Pennsylvania State University, University Park, Pennsylvania, USA 16802
F.E. Gildow
Affiliation:
Department of Plant Pathology, Pennsylvania State University, University Park, Pennsylvania, USA 16802
S.J. Fleischer*
Affiliation:
Department of Entomology, Pennsylvania State University, University Park, Pennsylvania, USA 16802
D. Cox-Foster
Affiliation:
Department of Entomology, Pennsylvania State University, University Park, Pennsylvania, USA 16802
F.L. Lukezic
Affiliation:
Department of Plant Pathology, Pennsylvania State University, University Park, Pennsylvania, USA 16802
*
1 Author to whom all corresponding should be addressed (E-mail: sjf4@psu.edu).
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Abstract

We describe the morphology of the alimentary canal in adult Acalymma vittata (F.), the vector of Erwinia tracheiphila (Smith) Bergey et al. emend. Hauben et al. (Enterobacteriaceae), the causal agent of bacterial wilt in cucurbits (Cucurbitaceae). The foregut includes a pre-oral cavity, pharynx, oesophagus, and crop but lacks a well-developed proventriculus. The midgut occupies approximately 65% of the length of the gut, has distinctive ventricular crypts throughout its length, and is lined with a peritrophic membrane, but lacks caeca for harboring symbionts. The hindgut comprises the colon and rectum and four Malpighian tubules. The cuticular intima of both foregut and hindgut bears rows of spines and is thrown into numerous folds. Transmission electron microscopy showed bacteria resembling E. tracheiphila within the hindgut 1 and 30 d after the beetles fed on E. tracheiphila spread between cotyledons of cucumber, Cucumis sativus L. (Cucurbitaceae). Our observations suggest that the midgut is not appropriate for long-term retention of E. tracheiphila because of the absence of caeca and the presence of a peritrophic membrane. Temporary and long-term pathogen retention may be associated with rows of spines and numerous folds within the foregut and hindgut.

Résumé

On trouvera ici la description de la morphologie du canal alimentaire de l’adulte d’Acalymma vittata (F.), le vecteur d’Erwinia tracheiphila (Smith) Bergey et al. emend Hauben et al. (Enterobacteriaceae), l’agent de la pourriture bactérienne des cucurbitacés (Curcubitaceae). L’intestin antérieur comporte une cavité pré-orale, le pharynx, l’oesophage et le gésier, mais il n’y a pas de proventricule bien développé L’intestin moyen occupe environ 65% de la longueur du tube digestif, comporte des cryptes ventriculaires distinctives sur toute sa longueur et est tapissé d’une membrane péritrophique; il n’y a cependant pas de caecums abritant des symbiontes. L’intestin postérieur est formé du colon, du rectum et de quatre tubules de Malpighi. La couche cuticulaire interne des intestins antérieur et postérieur porte des rangées d’épines et forme de nombreux replis. Le microscope électronique a démontré la présence de bactéries semblables à E. tracheiphila dans l’intestin postérieur des coléoptères 1 et 30 jours après leur consommation d’E. tracheiphila répandus entre les cotylédons du concombre, Cucumis sativus L. (Cucurbitaceae). Nos observations indiquent que l’intestin moyen n’est pas un bon site de préservation à long terme de la bactérie à cause de l’absence de caecums et de la présence d’une membrane péritrophique. La rétention temporaire ou à long terme du pathogène est peut-être associée aux multiples rangs d’épines et replis dans l’intestin antérieur et l’intestin moyen.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 132 , Issue 1 , February 2000 , pp. 1 - 13
Copyright
Copyright © Entomological Society of Canada 2000

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