Hostname: page-component-745bb68f8f-b6zl4 Total loading time: 0 Render date: 2025-01-10T23:57:49.760Z Has data issue: false hasContentIssue false
  • English
  • Français

BATCH SURFACE STERILIZATION OF CHIRONOMID EGGS

Published online by Cambridge University Press:  31 May 2012

B. E. Townsend  and
R. D. Hamilton
B. E. Townsend
Affiliation:
Freshwater Institute, Environment Canada, Winnipeg, Manitoba
R. D. Hamilton
Affiliation:
Freshwater Institute, Environment Canada, Winnipeg, Manitoba
Get access Rights & Permissions [Opens in a new window]

Abstract

A simple apparatus for the surface sterilization of a large number of chironomid eggs is described. The apparatus allows as many as 4000 eggs to be processed in 20 min without the inconvenience of manual transfer and washing procedures. Hatching percentages of untreated and treated eggs were 97.2% and 96.4% respectively. The hatching percentage of eggs produced by females that had developed from treated eggs was 96%. Comparisons between percentage emergence, sex ratios, adult viability, and fecundity of untreated and treated animals indicated that surface sterilization had little or no effect on chironomid development.

Type
Articles
Information
The Canadian Entomologist , Volume 108 , Issue 6 , June 1976 , pp. 669 - 671
Copyright
Copyright © Entomological Society of Canada 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Healy, F. P. 1973. Characteristics of phosphorous deficiency in Anabaena. J. Phycol. 9: 383–394.CrossRefGoogle Scholar
Lang, C. A., Basch, Karen J., and Store, R. S.. 1972. Growth composition and longevity of the axenic mosquito. J. Nutr. 120(8): 1057–1066.CrossRefGoogle Scholar
Lea, A. O., Dimond, J. B., and DeLong, D. M.. 1956. A chemically defined medium for rearing Aedes aegypti: Larvae. J. econ. Ent. 49(3): 313–315.CrossRefGoogle Scholar
Lichtenstein, E. P. 1948. Growth of Culex molestus under sterile conditions. Nature 4130: 999–1000.Google Scholar
Townsend, B. E. and Flannagan, J. F.. In press. Aspects of the biology of a laboratory population of Chironomus tentans cultured for use as a bioassay animal. Tech. Rep. Fish. Res. Bd Can.Google Scholar
Wallis, R. C., and Lite, S. W.. 1970. Axenic rearing of Culex salinarus. Mosquito News 30(3): 427–429.Google Scholar