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Identification, distribution, and molecular characterization of the apple aphids Aphis pomi and Aphis spiraecola (Hemiptera: Aphididae: Aphidinae)

Published online by Cambridge University Press:  02 April 2012

R.G. Foottit ,
D.T. Lowery ,
H.E.L. Maw ,
M.J. Smirle  and
G. Lushai
R.G. Foottit*
Affiliation:
Invertebrate Biodiversity, National Environmental Health Program, Agriculture and Agri-Food Canada, K.W. Neatby Building, 960 Carling Avenue, Ottawa, Canada ON K1A 0C6
D.T. Lowery
Affiliation:
Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, P.O. Box 4200, Highway 97, Summerland, Canada BC V0H 1Z0
H.E.L. Maw
Affiliation:
Invertebrate Biodiversity, National Environmental Health Program, Agriculture and Agri-Food Canada, K.W. Neatby Building, 960 Carling Avenue, Ottawa, Canada ON K1A 0C6
M.J. Smirle
Affiliation:
Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, P.O. Box 4200, Highway 97, Summerland, Canada BC V0H 1Z0
G. Lushai
Affiliation:
South West of England Regional Development Agency, Exeter, Devon EX1 1QA, United Kingdom
*
1Corresponding author (e-mail: Robert.Foottit@agr.gc.ca).
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Abstract

Morphometric techniques, DNA mitochondrial cytochrome c oxidase subunit 1 gene (COI) barcoding, and microsatellite flanking region sequences were used to assess the reliability of suggested morphological characters in distinguishing the green apple aphid (Aphis pomi De Geer) from the spirea aphid (Aphis spiraecola Patch), and to assess variation within these species. Both molecular approaches clearly distinguished two groups corresponding to the morphologically defined species. Differences in the length of the distal rostral segment and the number of lateral tubercles were found to be robust indicators of species membership, performing as well as multivariate approaches. Among A. pomi samples, microsatellite flanking region sequences were relatively uniform, whereas A. spiraecola exhibited much variability, which suggests that North American populations of the latter species are genetically much more complex.

Résumé

Des techniques morphométriques et l'utilisation de codes à barres d'ADN de COI et de séquences de la région flanquante des microsatellites nous ont servi à évaluer les caractères morphologiques qu'on a proposés pour distinguer le puceron vert du pommier (Aphis pomi De Geer) du puceron de la spirée (Aphis spiraecola Patch), et à déterminer la variation au sein de ces espèces. Les deux approches moléculaires permettent de distinguer clairement les deux groupes qui correspondent aux espèces définies morphologiquement. Les différences dans la longueur du segment distal du rostre et le nombre de tubercules latéraux sont des indicateurs robustes de l'identité spécifique; l'utilisation de ces caractères fonctionne aussi bien que les méthodes multidimensionnelles. Les séquences de la région flanquante des microsatellites sont relativement uniformes chez A. pomi, mais elles sont très variables chez A. spiraecola, ce qui laisse croire que les populations nord-américaines de cette dernière espèce sont génétiquement beaucoup plus complexes.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 141 , Issue 5 , October 2009 , pp. 478 - 495
Copyright
Copyright © Entomological Society of Canada 2009

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