Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-14T04:50:35.353Z Has data issue: false hasContentIssue false
  • English
  • Français

Nutritional Studies with Battella germanica (L.) Reared Under Aseptic Conditions: I. Equipment and Technique1

Published online by Cambridge University Press:  31 May 2012

Howard L. House  and
Robert L. Patton
Howard L. House
Affiliation:
Department of Entomology, New York State College of Agriculture, Cornell University, Ithaca, N.Y.
Robert L. Patton
Affiliation:
Department of Entomology, New York State College of Agriculture, Cornell University, Ithaca, N.Y.
Get access Rights & Permissions [Opens in a new window]

Extract

In biological research it is often desirable to rear and handle animals free from microorganisms. This is particularly true in nutritional studies in which it is generally recognized that certain orgnisms in the digestive tract can supply nutritional factors which might otherwise be lacking in the diet. The possibility of this intervention makes it necessary to devise techniques for rearing and handling insects aseptically, in so far is the fauna and flora of their intestinal tract and of their environment are concerned. Consequently, a technique used throughout the present series of studies was developed making use of specially designed equipment by means of which nymphs of the cockroach, Blattella germanica (L.), were hatched and maintained under aseptic conditions, fed aseptic food, and kept aseptic throughout a series of measurements to evaluate their weight increment. The equipment and methods used constitute the basis of the present paper.

Type
Articles
Information
The Canadian Entomologist , Volume 81 , Issue 4 , April 1949 , pp. 94 - 100
Copyright
Copyright © Entomological Society of Canada 1949

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anonymous. Manual of dehydrated culture media and reagents. 6th ed. Difco Laboratories Inc. Detroit, Mich. 1939.Google Scholar
Gier, H. T.The morphology and behaviour of the intracellular bacteroids of roaches. Biol. Bull., 71: 433–452. 1936.CrossRefGoogle Scholar
Gier, H. T.Intracellular bacteroids in the cockroach, Periplaneta americana, (Linn.). J. Bact., 53: 173–189. 1947.CrossRefGoogle ScholarPubMed
Glaser, R. A.On the isolation, cultivation and classification of the so-called intracellular “symbionts” or “rickettsia” of Periplaneta americana. J. Expt. Med., 51: 59–81. 1930.CrossRefGoogle ScholarPubMed
Reyniers, J. A.Micrurgical and germ-free methods. 1st ed.Charles C. Thomas, Springfield, Ill. 1943.Google Scholar
Steinhaus, E. A.Insect microbiology. Comstock Publishing Co., Inc., Ithaca, N.Y. 1946.Google Scholar
Wollman, E.Observations sur une lignée aseptique de blattes (Blattela germanica) datant de cinq ans. C. R. Soc. Biol. Paris, 95: 164–165. 1926.Google Scholar