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Primary structure and expression of vitellogenin A and vitellogenin B in the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)

Published online by Cambridge University Press:  17 October 2022

Ahmed Sayed Ahmed Open the ORCID record for Ahmed Sayed Ahmed [Opens in a new window]  and
Norbert H. Haunerland Open the ORCID record for Norbert H. Haunerland [Opens in a new window]
Ahmed Sayed Ahmed
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
Norbert H. Haunerland*
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
*
*Corresponding author. Email: haunerla@sfu.ca
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Abstract

Vitellogenins are the major yolk protein precursors found throughout the animal kingdom. In insects, the primary structures of vitellogenins have been determined in species from most major orders, except for Orthoptera, where only partial sequences have been reported. We amplified and sequenced the complete complementary DNA for two vitellogenins from the desert locust, Schistocerca gregaria (Orthoptera: Acrididae), which code for vitellogenin A and vitellogenin B, two proteins of approximately 200 kDa each. Both proteins are highly expressed at similar rates in adult females only, and their expression can be completely abolished by RNA interference–mediated silencing of the juvenile hormone receptor, methoprene-tolerant (Met). Homologous sequences were inferred from the genome of Locusta migratoria (Orthoptera: Acrididae), yielding complete coding sequences considerably larger than previously published. Vitellogenin A and vitellogenin B from S. gregaria are highly similar to their respective homologues from L. migratoria but show relatively low similarity between each other, suggesting that these genes originated from a common ancestor long before these species diverged.

Type
Research Paper
Information
The Canadian Entomologist , Volume 154 , Issue 1 , 2022 , e43
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Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the Entomological Society of Canada

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Footnotes

Subject editor: Jeremy deWaard

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