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Abrogating HSP Response Augments Cell Death Induced by As2O3 in Glioma Cell Lines

Published online by Cambridge University Press:  02 December 2014

Xueming Song ,
Zhiqiang Chen ,
Chunbo Wu  and
Shiguang Zhao
Xueming Song
Affiliation:
Department of Neurosurgery, 1st Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, P.R. China
Zhiqiang Chen
Affiliation:
Department of Neurosurgery, 1st Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, P.R. China
Chunbo Wu
Affiliation:
Department of Neurosurgery, 1st Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, P.R. China
Shiguang Zhao*
Affiliation:
Department of Neurosurgery, 1st Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, P.R. China
*
Department of Neurosurgery, 1st Affiliated Hospital of Harbin Medical University. No 23, Youzheng Street, Nangang District, Harbin, P.R. China, 150001.
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Abstract

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Objectives:

We previously reported that Arsenic trioxide (ATO) can inhibit glioma growth both in vitro and in vivo. While the use of ATO alone for solid tumor treatment sometimes was found to be ineffective which may be due to the protective pathways including heat shock proteins (HSPs) response induced by ATO. In this study, we modified HSPs expression to investigate whether HSPs had some effect on ATO induced glioma cell death.

Methods:

Trypan bule exclusion assay, mitochondrial membrane potential (MMP) Assay, and SubG1 detection were used to evaluate cell viability and western-blot was employed to detect HSPs and some apoptosis markers expression induced by ATO. Heat pre-treatment, HSPs inhibitor, or Heat Shock factor-1 (HSF1) knockdown by SiRNA was employed to modify HSPs levels.

Results:

It was showed that KNK437 (HSPs inhibitor) or HSF1 knockdown significantly enhanced cell death, MMP disruption, JNK phosphorylation and caspase-3 cleavage induced by ATO, which was accompanied by abrogation of HSPs induction, while heat pre-treatment with clear HSPs induction had strong protection on the effects mentioned above.

Conclusion:

Those data suggested that HSPs play protective roles on ATO induced cell death in glioma. Inhibition of HSPs may have a synergistic effect with ATO on glioma treatment.

Type
Original Article
Information
Canadian Journal of Neurological Sciences , Volume 37 , Issue 4 , July 2010 , pp. 504 - 511
Copyright
Copyright © The Canadian Journal of Neurological 2010

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