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Acute Spinal Cord Injury in the Rat: Comparison of Three Experimental Techniques

Published online by Cambridge University Press:  18 September 2015

Moe Khan  and
Robert Griebel
Moe Khan*
Affiliation:
Department of Clinical Neurological Sciences, Division of Neurosurgery, University of Saskatchewan, Saskatoon
Robert Griebel
Affiliation:
Department of Clinical Neurological Sciences, Division of Neurosurgery, University of Saskatchewan, Saskatoon
*
Dept. of Clinical Neurological Sciences, University Hospital, Saskatoon, Saskatchewan S7N 0X0 Canada.
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Three techniques to produce experimental spinal cord injuries in the rat are compared; 1) the weight dropping method, 2) the aneurysm clip compression method and 3) the extradural balloon compression method. In principle, different forces were used in technique one, while a constant force for different durations is maintained in techniques two and three. The relationship between these different types of injuries and subsequent clinical recovery was assessed quantitatively by the inclined plane method of Rivlin and Tator. The weight dropping technique was found unreliable for experimental spinal cord injury in the rat while the aneurysm clip compression technique resulted in consistent cord injuries with respect to subsequent clinical recovery. The extradural balloon compression method invariably resulted in complete recovery after three and five minutes but no recovery after seven minutes of 0.1 cc air inflated balloon compression of the cord indicating a steep dose — response curve. However, using a 0.2 cc air inflated balloon, no recovery was noted after one minute compression. The major factor in the pathogenesis of spinal cord injury produced by the weight dropping technique is believed to be mechanical, while both mechanical and vascular factors seem to operate in the clip and balloon compression techniques.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 10 , Issue 3 , August 1983 , pp. 161 - 165
Copyright
Copyright © Canadian Neurological Sciences Federation 1983

References

Albin, M.S., White, R.J., and Locke, G.A., et al (1967). Localized spinal cord hypothermia: anesthetic effects and applications to spinal cord injury. Anesth Analg 46:8–16.CrossRefGoogle Scholar
Allen, A.R. (1911). Surgery of experimental lesion of spinal cord and equivalent to crush injury of fracture dislocation of spinal column. Preliminary Report. JAMA 57:878–880.CrossRefGoogle Scholar
Daniell, H.B., Wooford, W.F., Lee, W.A. and Ducker, T.B. (1975). A method of quantitating injury inflicted on acute spinal cord studies. Paraplegia 13:137–142.Google ScholarPubMed
Dohrmann, C.J., Panjabi, M.M., and Wagner, F.C., (1976). An apparatus for quantitating experimental spinal cord trauma. Surg Neurol 5:315–318.Google ScholarPubMed
Eidelberg, E.M., Staten, E., and Watkins, A.C., et al (1976). A model of spinal cord injury. Surg Neurol, 6:35–38.Google Scholar
Freeman, L.W., and Wright, T.W. (1953). Experimental observations of concussion and contusion of the spinal cord. Ann Surg 137:433–443.CrossRefGoogle ScholarPubMed
Hansebout, R.P., Eugene, F., Kuthner, F., and Romero-Sierra, C., (1975). Effects of local hypothermia and of steroids upon recovery from experimental spinal cord compression injury. Surg Neurol 4:531–536.Google ScholarPubMed
Hedeman, L.S., Shellenberger, M.K, and Gordon, J. H., (1974). Studies in experimental spinal cord trauma. Part 1 : Alterations in catecholamine levels. J Neurosurg 40:429–434.Google Scholar
Hung, T.K., Albin, M.S., and Brown, T.D., et al (1975). Biomechanical responses to open experimental spinal cord injury. Surg Neurol 4:271–276.Google ScholarPubMed
Kajihara, K., Kawanga, H., and de la Torre, J.C., et al (1973). Demethyl sulfoxide in the treatment of experimental spinal cord injury. Surg Neurol 1:16–22.Google Scholar
Koozekanani, S.H., Vise, W.M., Hashemi, R.M., and McGhee, R.B., (1976). Possible mechanisms for observed pathophysiological variability in experimental spinal cord injury by the method of Allen. J Neurosurg 44:429–434.CrossRefGoogle ScholarPubMed
Martin, S.H., and Bloedel, J.R., (1973). Evaluation of experimental spinal cord injury using cortical evoked potentials. J Neurosurg 39:75–81.CrossRefGoogle ScholarPubMed
Naftchi, N.E., Demeny, M., and DeCrescito, V., et al (1974). Biogenic amine concentrations in traumatized spinal cords of cats. Effect of drug therapy. J Neurosurg 40:52–57.CrossRefGoogle ScholarPubMed
Osterholm, J.L., (1974). The pathophysiological response to spinal cord injury. The current status of related research. J Neurosurg 40:5–33.CrossRefGoogle ScholarPubMed
Riese, W. (1959). A history of Neurology. M.D. Publications, N.Y., pp. 223.Google Scholar
Rivlin, A.S., and Tator, C.H., (1978). Effect of duration of acute spinal cord compression in a new acute model in the rat. Surg Neurol 10:39–43.Google Scholar
Rivlin, A.S., and Tator, C.H., (1977). Objective clinical assessment of motor function after experimental spinal cord injury in the rat. J Neurosurg 47:577–581.CrossRefGoogle ScholarPubMed
Siegel, R.E., (1973). Galen on Psychology, Psychopathology and function and diseases of the nervous system. Kanger Basel, S., IX + 310.Google Scholar
Tarlov, I.M.Spinal cord compression. Springfiled, Ill., Charles C. Thomas, 1957.Google Scholar
Tator, C.H., (1973). Acute spinal cord injury in primates produced by an inflatable extradural cuff. Canad J Surg 16:222–231.Google ScholarPubMed
White, R.J., Albin, M.S., and Harris, L.S., et al (1969). Spinal cord injury: sequential morphysiology and hypothermia stabilization. Surg Forum 20:432–434.Google Scholar