Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T07:55:58.847Z Has data issue: false hasContentIssue false
  • English
  • Français

The Effects of Myoinositol on the Autonomic Neuropathy in the Streptozotocin Diabetic Rat - A Freeze Fracture Study

Published online by Cambridge University Press:  18 September 2015

G. Monckton  and
H. Marusyk
G. Monckton*
Affiliation:
Muttart-Collip Memorial E.M. Laboratory, Division of Neurology, Department of Medicine, University of Alberta, Edmonton, Alberta
H. Marusyk
Affiliation:
Muttart-Collip Memorial E.M. Laboratory, Division of Neurology, Department of Medicine, University of Alberta, Edmonton, Alberta
*
522 Medical Arts Building, 11010 Jasper Avenue, Edmonton, Alberta, Canada T5K 0K9
Rights & Permissions [Opens in a new window]

Abstract:

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

This report describes a freeze fracture study of rat dorsal sympathetic chain in normal, streptozotocin diabetic on normal diet, and a group fed a 1% myoinositol normal diet. In nonmyelinated fibres, the diabetic group had significant loss of particles on the P face ofjuxta-axonal Schwann cell membranes, whilst myelinated nerves showed a profound loss of particles on P and E faces. In the group with adjuvant myoinositol the particle numbers were normal in both types of nerve. Axonal plasma membranes showed increased numbers of particles in the P face of the nonmyelinated membranes in the diabetic, but were normal in the myoinositol group. Myelinated axonal membranes showed no significant difference between the diabetic and the normal. Rats on a myoinositol diet showed a significant decrease in the E face particles. These results are consistent with those described in the sciatic myelinated nerves by earlier authors and reinforce the view that myoinositol is an important membrane constituent as phosphatidyhnositol and is essential for the expression of normal protein particle numbers.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 15 , Issue 2 , May 1988 , pp. 147 - 151
Copyright
Copyright © Canadian Neurological Sciences Federation 1988

References

REFERENCES

1.Monckton, G, Pehowich, E. Autonomic neuropathy in the streptozotocin diabetic rat. Can J Neurol Sci 1980; 7: 135–142.CrossRefGoogle ScholarPubMed
2.Fukuma, M, Carpentier, J-L, Orci, L, et al. An alteration in internodal myelin membrane structure in large sciatic nerve fibres in rats with acute streptozotocin diabetes and impaired nerve conduction velocity. Diabetologia 1978; 15: 65–72.CrossRefGoogle ScholarPubMed
3.Pinto-da Silva, P, Branton, D. Membrane splitting in freeze-fracture. J Cell Biol 1975; 45: 598–605.CrossRefGoogle Scholar
4.Verkleij, PJ. Lipidie intramembranous particles. Biochem Biophys Acta 1984; 779: 43–63.Google Scholar
5.Rosenbluth, J. Intramembranous particle distribution in nerve fiber membranes. Experientia 1983; 39: 953–963.CrossRefGoogle ScholarPubMed
6.Engel, AD, Fukugana, H, Osame, M. Stereometric estimation of the area of the freeze-fractured membrane. Muscle Nerve 1982; 5: 682–685.CrossRefGoogle Scholar
7.Greene, DA, DeJesus, PV Jr, Winegrad, AI. Effects of insulin and dietary myoinositol on impaired peripheral motor nerve conduction velocity in acute streptozotocin diabetes. J Clin Invest 1975; 55: 1326–1336.CrossRefGoogle ScholarPubMed
8.Greene, DA, Lattimer, S, Sima, AF. Sorbitol, phosphoinositides and sodium-potassium. ATPase in the pathogenesis of diabetic complications. New Engl J Med 1987; 316: 599–606.Google ScholarPubMed
9.Sima, AF, Lattimer, SA, Yagihashi, S, Greene, DA. Axoglial dysfunction: A novel structural lesion that accounts for poorly reversible slowing of nerve conduction in the spontaneously diabetic bio-breeding rat. J Clin Invest 1986; 77: 474–484.CrossRefGoogle ScholarPubMed
10.Mayer, JH, Tomlinson, DR. Prevention of defects of axonal transport and nerve conduction velocity by oral administration of myoinositol or an aldose reductase inhibitor in streptozotocin diabetic rats. Diabetologia 1983; 25: 433–438.CrossRefGoogle ScholarPubMed