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Gliosis and Glioma Distinguished by Acridine Orange

Published online by Cambridge University Press:  18 September 2015

Harvey B. Sarnat ,
Bernadette Curry ,
N.B. Rewcastle  and
Cynthia L. Trevenen
Harvey B. Sarnat*
Affiliation:
Departments of Pathology,Paediatrics, and Clinical Neurosciences, University of Calgary Faculty of Medicine, Calgary
Bernadette Curry
Affiliation:
Departments of Pathology,Paediatrics, and Clinical Neurosciences, University of Calgary Faculty of Medicine, Calgary
N.B. Rewcastle
Affiliation:
Departments of Pathology,Paediatrics, and Clinical Neurosciences, University of Calgary Faculty of Medicine, Calgary
Cynthia L. Trevenen
Affiliation:
Departments of Pathology,Paediatrics, and Clinical Neurosciences, University of Calgary Faculty of Medicine, Calgary
*
Alberta Children's Hospital, 1820 Richmond Road S.W., Calgary, Alberta, Canada T2T 5C7
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Abstract:

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Acridine orange fluorochrome of nucleic acids was applied to sections of cerebral tissue from 20 patients showing acute or chronic reactive gliosis. The results were compared with the findings in 39 well differentiated and malignant astrocytomas. The orange cytoplasmic fluorescence of ribonucleic acid is lacking in reactive astrocytes of all ages including gemistocytes, but is uniformly present in astrocytoma cells. Acridine orange is a useful supplementary stain for distinguishing between astrocytosis and astrocytoma, paticularly for small cerebral biopsies showing scattered or diffusely infiltrating pleomorphic glial cells.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 14 , Issue 1 , February 1987 , pp. 31 - 35
Copyright
Copyright © Canadian Neurological Sciences Federation 1987

References

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