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Secondary Epileptogenesis in Frog Forebrain: Effect of Inhibition of Protein Synthesis

Published online by Cambridge University Press:  18 September 2015

F. Morrell ,
N. Tsuru ,
T.J. Hoeppner ,
D. Morgan  and
W.H. Harrison
F. Morrell*
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
N. Tsuru
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
T.J. Hoeppner
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
D. Morgan
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
W.H. Harrison
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
*
Rush-Presbyterian - St. Lukes Medical Center, Chicago, 111, 60612 U.S.A.
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Secondary epileptogenesis was induced in the hippocampal cortex of the paralyzed bullfrog by means of localized, unilateral, intermittent electrical stimulation (kindling). Stimuli were designed to yield a brief but definite after-discharge. In control animals a progressive increase in after-discharge duration occurred at the 1° (stimulated) site and then at the 2° site (contralateral hippocampus). Spontaneous epileptiform potentials (SEP's) occurred between stimuli, eventually independently on both sides.

Cycloheximide (50 mg/kg) caused 88-99% reduction in protein synthesis, measured by 14C-leucine incorporation into brain protein. Cycloheximide-treated animals revealed no evidence of progressive prolongation of after-discharge duration when subjected to the kindling procedure (p = 0.1205xl0-7). SEP's were reduced in the cycloheximide-treated animals, and confined to 1° hemisphere (p=0.6xl0-10).

Since cycloheximide did not disturb normal electrogenesis or disrupt the after-discharges, this experiment distinguishes processes dependent upon electrical events from those requiring macromolecular synthesis. Protein synthesis seems critical to the emergence of spontaneous and autonomous epileptiform behavior of neural aggregates.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 2 , Issue 4 , November 1975 , pp. 407 - 416
Copyright
Copyright © Canadian Neurological Sciences Federation 1975

References

REFERENCES

Agranoff, B.W. (1968). Biological effects of antimetabolites used in behavioral studies. In: D. H. Efron et al. eds “Psychopharmacology: A Review of Progress, 1957-1967.” PHS Publ. 1836, pp. 909–917. Wash., D.C. U.S. Govt. Printing Off.Google Scholar
Barondes, S.H. and Cohen, H.D. (1968). Memory impairment after subcutaneous injection of acetoxycycloheximide. Science 160: 556.CrossRefGoogle ScholarPubMed
Brink, J.J., Davis, R.E. and Agranoff, B.W. (1966). Effects of Puromycin, Acetoxycycloheximide and Actinomycin D on protein synthesis in goldfish brain. J. Neurochem. 13: 889journal896.CrossRefGoogle ScholarPubMed
Corcoran, M.E., Fibiger, H.C.McCaughran, J.A. Jr. and Wada, J.A. (1974). Potentiation of amygdaloid kindling and Metrazol-induced seizures by 6-Hydroxydopamine in rats. Exptl. Neurol. 45: 118–133.CrossRefGoogle ScholarPubMed
Farber, J.L. and Farmar, R. (1973). Differential effects of cycloheximide on protein and RNA synthesis as a function of dose. Biochem. Biophy. Res. Commun. 51: 626–630.CrossRefGoogle ScholarPubMed
Flexner, L.B., Flexner, J.B. and Roberts, R.B. (1966). Stages of memory in mice treated with acetoxycycloheximide before or immediately after learning. Proc. Nat. Acad. Sci. USA 56: 730–735.CrossRefGoogle ScholarPubMed
Flexner, L.B., Serota, R.G. and Goodman, R.H. (1973). Cycloheximide and Acetoxycycloheximide: Inhibition of Tyrosine Hydroxylase activity and amnestic effects. Proc. Nat. Acad. Sci. USA 70: 354–356.CrossRefGoogle ScholarPubMed
Goddard, G.V. (1967). The development of epileptic seizures through brain stimulation at low intensity. Nature 214: 1020.CrossRefGoogle ScholarPubMed
Goddard, G.V., McIntyre, D. and Leech, C. (1969). A permanent change in brain function resulting from daily electrical stimulation. Exp. Neurol. 25: 295–330.CrossRefGoogle ScholarPubMed
Goddard, G.V. and Morrell, F. (1971). Chronic progressive epileptogenesis induced by focal electrical stimulation of brain. Neurol. 21: 393.Google Scholar
Guerrero-Figueroa, R., Debalb1An, V.G., Barros, A. and Heath, R.G. (1964a). Cholinergic mechanism in subcortical mirror focus and effects of topical application of gamma aminobutyric acid and acetylcholine. Epilepsia 5: 140–155.CrossRefGoogle ScholarPubMed
Guerrero-Figueroa, R., Barros, A. and Heath, R.G. (1964b). Experimental subcortical epileptiform focus. Epilepsia 5: 112–139.CrossRefGoogle ScholarPubMed
Holubar, J., Strejckova, A. and Servit, Z. (1966). Penicillin and mirror epileptogenic foci in the brain hemisphere of the frog. In: Comparative and Cellular Pathophysiology of Epilepsy, edited by Servit, Z. Excerpta Medica International Congress Series 124, Amsterdam. 214–220.Google Scholar
Lovell, R. (1971). Some neurochemical aspects of convulsions. In: Handbook of Neurochemistry. Vol. 6: Alterations of Chemical Equilibrium in the Nervous System. Lajtha, A. ed. Plenum Press, New York, pp. 63–102.CrossRefGoogle Scholar
Maynert, E.W. (1969). The role of biochemical and neurochemical factors in the laboratory evaluation of antiepileptic drugs. Epilepsia 10: 145–162.CrossRefGoogle Scholar
Morrell, F. (1959). Experimental focal epilepsy in animals. Arch. Neurol. 1: 141–147.CrossRefGoogle ScholarPubMed
Morrell, F. (1960). Secondary epileptogenic lesions. Epilepsia 1: 538–560.CrossRefGoogle ScholarPubMed
">Morrell, F. (1961). Electrochemical mechanisms and information storage in nerve cells. M.l.T. Seminar-Lecture Series, In: Molecular Specificity and Biological Memory, Schmitt, F.O. Ed. pp. 73–79.Google Scholar
Morrell, F. (1961). Lasting changes in synaptic organization produced by continuous neuronal bombardment. In: CIOMS Conference on Brain Mechanisms and Learning, Delafresnaye, J.F.Fessard, A. and Konorski, J. (Eds.), Blackwell Scientific Publications, Oxford, pp. 375–392.Google Scholar
Morrell, F. (1969). Physiology and Histochemistry of the Mirror Focus. In: Basic Mechanisms of the Epilepsies. Eds.: Jasper, H.H.Ward, A.A. and Pope, A.Little, Brown Co., Boston, pp. 357–370.Google Scholar
Morrell, F. (1973). Goddard‘s Kindling Phenomenon: A New Model of the “Mirror Focus”, Chemical Modulation of Brain Function, Edited by H.C., SabelliRaven Press, New York, pp. 207–223.Google Scholar
Morrell, F. (1974). Animal Models of Human Focal Epilepsy. In: Klawans, H.L. ed. Models of Human Neurological Diseases, Excerpta Medica, Amst. pp. 167–199.Google Scholar
Morrell, F. and Baker, L. (1961). Effect of drugs on secondary epileptogenic lesions. Neurology 11: 651–664.CrossRefGoogle Scholar
Morrell, F. and Florenz, A. (1958). Modifications of the freezing technique for producing experimental epileptogenic lesions. Electroenceph. clin. Neurophysiol. 10: 187.Google Scholar
Morrell, F., Proctor, F. and Prince, D.A. (1965). Epileptogenic properties of subcortical freezing. Neurology 15:744–751.CrossRefGoogle ScholarPubMed
Morrell, F., Sandler, B. and Ross, G. (1959). The “mirrorfocus” as a model of neural learning. Proc. XXI Internat. Congress Physiol. Sciences, Buenos Aires, p. 193.Google Scholar
Morrell, F. and Tsuru, N. (1974). Development of spontaneous hypersynchrony in the hippocampal cortex of the bullfrog, Rana catesbeiana. The Biological Bulletin, 147: 492.Google Scholar
Morrell, F. and Tsuru, N. (1975). Kindling in the Frog: Development of Spontaneous Epileptiform Activity. Electroencephalog. clin. Neurophsyiol. In Press.Google Scholar
Obrig, T.G., Culp, W.J., Mckeehan, W.C. and Hardesty, B. (1971). The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes. J. Biol. Chem. 246: 174–181.CrossRefGoogle ScholarPubMed
Pinel, J.P.J., Phillips, A.G. and Deol, G. (1974). Effects of current intensity on kindled motor seizure activity in rats. Behav. Biolq. 11: 59–68.CrossRefGoogle ScholarPubMed
Purpura, D.P., Penry, J.K., Tower, D.B., Woodbury, D.M. and Walter, R.D. editors (1972). Experimental Models of Epilepsy — A Manual for the Laboratory Worker. Raven Press, New York. pp. 615.Google Scholar
Racine, R.J. (1972a). Modification of seizure activity by electrical stimulation. I. After-discharge threshold. Electroenceph. clin. Neurophysiol. 32: 269–279.CrossRefGoogle ScholarPubMed
Racine, R.J. (1972b). Modification of seizure activity by electrical stimulation. II. Motor Seizure, Electroenceph. clin. Neurophysiol. 32, 281–294.CrossRefGoogle ScholarPubMed
Racine, R.J. (1975). Modification of seizure activity by electrical stimulation: cortical areas. Electroenceph. clin. Neurophysiol. 38: 1–12.CrossRefGoogle ScholarPubMed
Racine, R.J., Livingston, K. and Joaquin, A. (1975). Effects of procaine hydrochloride, diazepam and diphenylhydantoin on seizure development in cortical and subcortical structures in rats. Electroencephalog. clin. Neurophysiol. 38: 355–365.CrossRefGoogle ScholarPubMed
Tanaka, A. (1972). Progressive changes of behavioral and electroencephalographic responses to daily amygdaloid stimulations in rabbits. Fukuoka Acta Med. 63: 152–164.Google Scholar
Tress, K.H. and Herberg, L.J. (1972). Permanent reduction in seizure threshold resulting from repeated electrical stimulation. Exp. Neurol. 37: 347–359.CrossRefGoogle ScholarPubMed
Wada, J.A. (1964). Longitudinal analysis of chronic epileptogenic brain process. In: Wada, T. (ed). Epileptology-Clinical and Basic Aspects. Igaku Shoin Ltd. Tokyo, p. 426–453.Google Scholar
Wada, J. and Cornelius, L. (1960). Functional alterations of deep structures in cats with chronic irritative lesions. Arch. Neurol. 3: 425–447.CrossRefGoogle ScholarPubMed
Wada, J.A. and Sato, M. (1974) Generalized convulsive seizures induced by daily electrical stimulation of the amygdala in cats. Neurology 24: 565–574.CrossRefGoogle ScholarPubMed
Wada, J., Sato, M. and Green, J. (1974). Anticonvulsive and prophylactic potency of antiepileptic agents: Assessment by amygdaloid kindling preparations in cats. Epilepsia 15: 276.qGoogle Scholar
Wilcox, G.L. Jr., Andry, D.K. and Luttges, M.W. (1974). Cycloheximide effects on electroencephalographic and evoked responses in mice. Behavioral Biology 12: 81–92.CrossRefGoogle ScholarPubMed
Wilder, B.J. (1972). Projection Phenomena and Secondary Epileptogenesis — Mirror Foci. In: Experimental Models of Epilepsy — A Manual for the Laboratory Worker. Purpura, D.p.Penry, J.K.D.B., TowerWoodbury, D.M.Walter, R.D. eds. Raven Press, New York, 85–111.Google Scholar
Wilder, B.J. and Morrell, F. (1967a). Secondary epileptogenesis in the frog forebrain. Neurology 17: 1041–1051.CrossRefGoogle ScholarPubMed
Wilder, B.J. and Morrell, F. (1967b). Cellular behavior in secondary epileptic lesions. Neurology, 17: 1193–1204.CrossRefGoogle ScholarPubMed