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Bone morphogenetic protein receptor IB as a candidate gene for prolificacy in Small Tail Han and Hu ewes

Published online by Cambridge University Press:  13 June 2008

Yan Ya-Dong ,
Chu Ming-Xing ,
Zeng Yong-Qing ,
Fang Li ,
Ye Su-Cheng ,
Wang Li-Min ,
Guo Qing-Kun ,
Han Dai-Qin ,
Zhang Zhao-Xin  and
Wang Xi-Jun
...Show all authors
Yan Ya-Dong
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
Chu Ming-Xing*
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Zeng Yong-Qing
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
Fang Li
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Ye Su-Cheng
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Wang Li-Min
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Guo Qing-Kun
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Han Dai-Qin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Zhang Zhao-Xin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Wang Xi-Jun
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Zhang Zhao-Xin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
*
*Corresponding author. Email: mxchu@263.net
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Abstract

The bone morphogenetic protein receptor IB (BMPR-IB) gene, which controls the fecundity of Booroola Merino ewes, was studied as a candidate gene for the prolificacy of Small Tail Han and Hu ewes. A single nucleotide polymorphism of the BMPR-IB gene was detected in both high (Small Tail Han and Hu) and low (Suffolk and Dorset) fecundity sheep breeds by polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) analysis. The results indicated the presence of the same mutation (A746G) of the BMPR-IB gene in both Small Tail Han and Hu ewes and in Booroola Merino ewes, but not in both Suffolk and Dorset ewes. In Small Tail Han ewes, frequencies of BB, B+ and ++ genotypes were 0.524, 0.383 and 0.093, respectively. In Hu ewes, these frequencies were 0.882, 0.118 and 0.000. The BMPR-IB genotype distributions were significantly different (P<0.001) among high- and low-fecundity sheep breeds. Small Tail Han ewes with genotype BB had 0.92 (P<0.01) and 1.02 (P<0.01) lambs more than those with genotype ++ in the first and second parity, respectively. These results demonstrated that the BMPR-IB gene is a major gene affecting the prolificacy in both Small Tail Han and Hu ewes, and could be used as a molecular genetic marker to select the litter size in sheep.

Keywords

sheepprolificacybone morphogenetic protein receptor IB genePCR-SSCP
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 125 - 130
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Abdulkhaliq, AM, Harvey, WR and Parker, CF (1989) Genetic parameters for ewe productivity traits in the Columbia, Suffolk and Targhee breeds. Journal of Animal Science 67: 3250–3257.CrossRefGoogle ScholarPubMed
Chu, MX and Wang, XL (2001) Estimation of phenotypic and genetic parameters for birth type and number of lambs born alive in Hu sheep. Acta Genetica Sinica 28: 418–423 in Chinese with English abstractGoogle ScholarPubMed
Davis, GH, Montgomery, GW, Allison, A et al. , (1982) Segregation of a major gene influencing fecundity in progeny of Booroola sheep. New Zealand Journal of Agricultural Research 25: 525–529.CrossRefGoogle Scholar
Davis, GH, Galloway, SM, Ross, IK et al. , (2002) DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biology of Reproduction 66: 1869–1874.CrossRefGoogle ScholarPubMed
Galloway, SM, McNatty, KP, Cambridge, LM et al. , (2000) Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics 25: 279–283.CrossRefGoogle Scholar
Hu, JP, Bai, LQ, Zhang, QF et al. , (1992) Studies on genetic and phenotypic parameters of the prolificacy and lambskin traits in Hu sheep. Journal of Zhejiang Agricultural University 18: 39–43 (in Chinese with English abstract).Google Scholar
Lan, ZJ, Gu, P, Xu, X et al. , (2003) GCNF-dependent repression of BMP-15 and GDF-9 mediates gamete regulation of female fertility. EMBO Journal 22: 4070–4081.CrossRefGoogle ScholarPubMed
Lawson, KA, Dunn, NR, Roelen, BA et al. , (1999) Bmp4 is required for the generation of primordial germ cells in the mouse embryo. Genetics Development 13: 424–436.Google ScholarPubMed
Liu, SF, Jiang, YL and Du, LX (2003) Studies of BMPR-IB and BMP15 as candidate genes for fecundity in Little Tailed Han sheep. Acta Genetica Sinica 30: 755–760 (in Chinese with English abstract).Google ScholarPubMed
Mohd-Yusuff, MK, Dickerson, GE and Young, LD (1992) Reproductive rate and genetic variation in composite and parental populations: experimental results in sheep. Journal of Animal Science 70: 673–688.CrossRefGoogle ScholarPubMed
Monget, P, Fabre, S, Mulsant, P et al. , (2002) Regulation of ovarian folliculogenesis by IGF and BMP system in domestic animals. Domestic Animal Endocrinology 23: 139–154.CrossRefGoogle ScholarPubMed
Mulsant, P, Lecerf, F, Fabre, S et al. , (2001) Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. Proceedings of the National Academy of Sciences of the United States of America 98: 5104–5109.CrossRefGoogle ScholarPubMed
Piper, LR and Bindon, BM (1982) The Booroola Merino and the performance of medium non-Peppin crosses at Armidale. In: Piper, LR, Bindon, BM and Nethery, RD (editors) The Booroola Merino. Melbourne, Australia: CSIRO, pp. 9–20.Google Scholar
Shimasaki, S, Zachow, RJ, Li, D et al. , (1999) A functional bone morphogenetic protein system in the ovary. Proceedings of the National Academy of Sciences of the United States of America 96: 7282–7287.CrossRefGoogle ScholarPubMed
Souza, CJH, MacDougall, C, Campbell, BK et al. , (2001) The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene. Journal of Endocrinology 169: R1–R6.CrossRefGoogle Scholar
Tu, YR (1989) The Sheep and Goat Breeds in China. Shanghai: Shanghai Science and Technology Press, pp. 50–52, 55–58 (in Chinese).Google Scholar
Wang, GL, Mao, XZ, Davis, GH et al. , (2003a) DNA tests in Hu sheep and Han sheep (small tail) showed the existence of Booroola (FecB) mutation. Journal of Nanjing Agricultural University 26: 104–106 (in Chinese with English abstract).Google Scholar
Wang, QG, Zhong, FG, Li, H et al. , (2003b) The polymorphism of BMPR-IB gene associated with litter size in sheep. Grass-feeding Livestock 2: 20–23 (in Chinese with English abstract).Google Scholar
Wilson, T, Wu, XY, Juengel, JL et al. , (2001) Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biology of Reproduction 64: 1225–1235.CrossRefGoogle ScholarPubMed
Xie, Z, Cheng, RH and Han, YG (1998) Analysis on the lambing effects of rams in Hu sheep. Journal of Nanjing Agricultural University 21: 77–81 (in Chinese with English abstract).Google Scholar
Yi, SE, LaPolt, PS, Yoon, BS et al. , (2001) The type I BMP receptor BmprIB is essential for female reproductive function. Proceedings of the National Academy of Sciences of the United States of America 98: 7994–7999.CrossRefGoogle ScholarPubMed