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Mutation breeding of Aspergillus niger strain LW-1 for high-yield β-mannanase production

Published online by Cambridge University Press:  01 October 2008

Zhang Shu-Fei ,
Song Jia-Huan ,
Wu Min-Chen ,
Sheng Jin-Ping  and
Li Jian-Fang
Zhang Shu-Fei
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Song Jia-Huan
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Wu Min-Chen*
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Sheng Jin-Ping
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Li Jian-Fang
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
*
*Corresponding author. E-mail: bioch@163.com
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Abstract

A parent strain of Aspergillus niger LW-1 producing β-mannanase, preserved in our laboratory, was isolated. A strain, N-9, was screened out and further treated with vacuum microwave irradiation and ethyl methane sulphonate (EMS). A mutant strain, E-30, producing a high and stable yield of β-mannanase was obtained through screening by solid-state cultivation on the basic fermentation medium and several generations of bevel subculture. Its enzyme activity (36 675 U/g) was increased by 2.15 times compared to that of A. niger LW-1 (17 048 U/g). The production of high-yield β-mannanase by E-30 remained stable when maintained at 4°C for 2 months.

Keywords

β-mannanaseAspergillus nigervacuum microwave irradiationethyl methane sulphonate
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 153 - 158
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 346–350

References

Banik, S, Bandyopadhyay, S and Ganguly, S (2003) Bioeffects of microwave – a brief review. Bioresource Technology 87: 155–159.CrossRefGoogle ScholarPubMed
Chai, PP, Jiang, ZQ, Li, LT and Ri Xia, BG (2005) Optimization of β-mannanase production by Bacillus subtilis WY45. Journal of China Agricultural University 10(3): 77–80 (in Chinese with English abstract).Google Scholar
Chen, YG, Li, MG, Xu, LH, Liu, ZX, Xia, ZY and Wen, ML (2005) New model of physical mutagenic methods and its application status at microbion breeding. Journal of Yangtze University 2(5): 46–48 (in Chinese with English abstract).Google Scholar
Clarke, JH, Davidson, K, Rixon, JE, et al. (2000) A comparison of enzyme-aided softwood paperpulp using combinations of xylanase, mannanase and alpha-galactosidase. Application of Microbiology and Biotechnology 53(6): 661–667.CrossRefGoogle Scholar
Hashimoto, Y and Fukumoto, J (1996) Studies on the enzyme treatment of coffee beans: purification of mannanase from Rhyzopus niveus and its action on coffee mannan. Nippon Nogeikagaku Kaishi 43: 317–322.CrossRefGoogle Scholar
Heck, JX, Soares, LHB and Ayub, MAZ (2005) Optimization of xylanase and mannanase production by Bacillus circulans strain BL53 on solid-state cultivation. Enzyme and Microbial Technology 37: 417–423.CrossRefGoogle Scholar
Jackson, ME, Geronian, K and Knox, A (2004) A dose–response study with the feed enzyme beta-mannanase in broilers provided with corn–soybean meal based diets in the absence of antibiotic. Poultry Science 83(12): 1992–1996.CrossRefGoogle ScholarPubMed
Juhasz, T, Szengyel, Z, Reczey, K, Siika-Aho, M and Viikari, L (2005) Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. Process Biochemistry 40: 3519–3525.CrossRefGoogle Scholar
Khanongnuch, C, Asada, K, Tsuruga, H, Ooi, T, Kinoshita, S and Lumyong, S (1998) β-Mannanase and xylanase of Bacillus subtilis 5H active for bleaching of crude pulp. Journal of Fermentation Bioengineering 5: 461–466.CrossRefGoogle Scholar
Li, JF, Wang, BL and Wu, MC (2002) Fermentation process of acidic β-mannanase from Aspergillus niger. Food and Fermentation Industries 28(9): 19–22 (in Chinese with English abstract).Google Scholar
Li, JH and Fang, J (2004) Breeding of hemicellulase overproducing strain and conditions for hemicellulase production. Journal of Wuxi Light Industry University 23(5): 48–52 (in Chinese with English abstract).Google Scholar
Li, YQ (2001) Study on the screening of high yield xylanase producing strain Aspergillus niger by microwave mutagenesis. Journal of Microwaves 17(1): 49–53 (in Chinese with English abstract).Google Scholar
Pettey, LA, Carter, SD, Senne, BW and Shriver, JA (2002) Effect of beta-mannanase addition to corn–soybean meal diets on growth performance, carcass traits, and nutrient digestibility of weanling growing–finishing pigs. Journal of Animal Science 80(4): 1012–1019.CrossRefGoogle ScholarPubMed
Schomburg, D and Salzmann, M (1991) Enzyme Handbook. Berlin: Springer-Verlag, pp. 1–5.CrossRefGoogle Scholar
Shazman, A, Mizrahi, S, Cogan, U and Shimoni, E (2007) Examining for possible non-thermal effects during heating in microwave oven. Journal of Food Chemistry 103: 444–453.CrossRefGoogle Scholar
Wu, XZ (1988) Industrial Production Technology of Enzymes. Gilin: Science and Technology Gilin Press, pp. 96–103 (in Chinese).Google Scholar