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AFLP markers for genomic DNA fingerprinting in pigs

Published online by Cambridge University Press:  12 February 2007

Ren Jun ,
Huang Lu-Sheng ,
Gary Evens ,
Ai Hua-Shui ,
Gao Jun ,
Chen Ke-Fei  and
Ding Neng-Shui
Ren Jun
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
Huang Lu-Sheng*
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
Gary Evens
Affiliation:
Department of Pathology, University of Cambridge, UK
Ai Hua-Shui
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
Gao Jun
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
Chen Ke-Fei
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
Ding Neng-Shui
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, China
*
*Corresponding author. Email: lh256@cam.ac.uk
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Abstract

AFLP provides an effective, rapid and economical tool for detecting a large number of polymorphic genetic markers that are highly reliable and reproducible, and are able to be genotyped automatically. The AFLP technique has been used extensively to detect genetic polymorphisms, evaluate and characterize breed resources, construct genetic maps and identify genes. In this paper, we describe the optimization of the AFLP technique for porcine genomic DNA fingerprinting, including the enzyme digestion, adapter ligation, preamplification, selective amplification, denatured PAGE, silver staining and multicolour fluorescent detection. Twenty-eight polymorphic markers were detected in the pooled genomic DNA of 44 pig breeds (populations) by E32/T32 primer combinations.

Keywords

piggenomeAFLP fingerprinting
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 9 - 12
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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References

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