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Analysis of microsatellite DNA markers of OarHH35 and BMS2508 in four goat breeds

Published online by Cambridge University Press:  20 March 2007

Ouyang Xu-Xiang ,
Si Qi-Shun ,
Huang Sheng-Qiang ,
Deng Zao-Fu ,
Liu He -Xiang ,
He De-Shi ,
Tan Sheng-Guo  and
Hu Shu-Guang
Ouyang Xu-Xiang*
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Si Qi-Shun
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Huang Sheng-Qiang
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Deng Zao-Fu
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Liu He -Xiang
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
He De-Shi
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Tan Sheng-Guo
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Hu Shu-Guang
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
*
*Corresponding author. E-mail: ouwxu@163.com
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Abstract

Two microsatellite markers OarHH35 and BMS2508, closely associated with high levels of reproduction in sheep, were analysed for polymorphisms in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat and Boer goat. The number of alleles for OarHH35 was 11, 8, 7 and 10, respectively, in the four goat breeds. That for BMS2508 was 9, 5, 6 and 7. The polymorphic information content (PIC) values for OarHH35/BMS2508 in the above-mentioned goats were respectively 0.8294/0.8047, 0.8399/0.6894, 0.7985/0.6910 and 0.8582/0.7688. By analysing the dominant effect of the two microsatellite markers on litter size of Xiangdong black goat, it was shown that microsatellite markers BMS2508 and OarHH35 significantly influenced (P<0.05) litter size of goats. At the OarHH35 locus, the least squares means (LSM) of genotype 135/135 bp was the highest (3.67 number born/litter). Moreover, LSM of genotypes 123/135 bp and 125/125 bp also reached 3.00 kids born/litter, indicating that alleles 135 bp and 125 bp exerted a significant positive effect on the litter size in Xiangdong black goat. At the BMS2508 locus, LSM of genotypes 132/145 bp and 93/132 bp for litter size was 4.00 and 3.25 kids born/litter respectively, but that of genotype 122/122 bp for litter size was only 1.00 kid/litter, so alleles 145 bp and 93 bp had significant positive effects on the litter size of Xiangdong black goat, and the allele 122 bp had a significantly negative effect.

Keywords

goat breedsmicrosatellite markerslitter size
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 217 - 222
Copyright
China Agricultural University and Cambridge University Press 2006

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