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Cloning and characterization of the light-inducible Gacab promoter from Gossypium arboreum

Published online by Cambridge University Press:  12 February 2007

Li Wei-Min ,
Wang Zhi-Xing ,
Pei Xin-Wu  and
Jia Shi-Rong
Li Wei-Min
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Zhi-Xing
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Pei Xin-Wu
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jia Shi-Rong*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author: Email: jiasr@mail.caas.net.cn
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Abstract

A 1009 bp promoter sequence of cab gene, which encodes chlorophyll a/b binding protein belonging to a class of light-inducible proteins, was cloned from Gossypium arboreum. Sequence analysis showed that it had no obvious homology with previously published cab promoters. The full-length Gacab promoter and 5′ deletions with length of 197, 504 and 779 bp were fused with gus (uid A) gene, respectively, and plant expression vectors were used for transformation of Nicotiana tabacum cv. NC89. β-Glucuronidase (GUS) histochemical assay of transgenic tobacco plants showed that GUS was expressed specifically in leaves and young green tissues. GUS was not detected in the leaves of transgenic plants grown in the dark for 6 days. However, it was highly expressed in the leaves of these plants after induction with light for another 6 days, demonstrating that the full-length Gacab promoter is a light-inducible promoter. Transient GUS expression in rice calli indicated that the expression level of Gacab504::gus was the highest and stronger than that of the CaMV 35S promoter, while expression was reduced for Gacab197::gus, Gacab779::gus and Gacab1009::gus constructs. This suggests that −197 bp to −1 bp is a basic promoter of Gacab, some positive regulatory elements may exist in −504 bp to −197 bp, and the fragment −1009 bp to −504 bp may contain negative elements.

Keywords

Gossypium arboretumchlorophyll a/b binding proteinGacab promoterlight-inducibleregulatory element
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 17 - 22
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Degenhardt, J and Tobin, EM (1996) A DNA binding activity for one of two closely defined phytochrome regulatory elements in an Lhcb promoter is more abundant in etiolated than in green plants. Plant Cell 8: 31–41.Google Scholar
Gidoni, D, Brosio, P, Bond-Nutter, D, Bedbrook, J and Dunsmuir, P (1989) Novel cis -acting elements in Petunia Cab gene promoters. Molecular & General Genetics 215: 337–344.CrossRefGoogle ScholarPubMed
Gotor, C, Romero, LC, Inouye, K, et al. (1993) Analysis of three tissue-specific elements from the wheat Cab-1 enhancer. Plant Journal 3: 509–518.CrossRefGoogle ScholarPubMed
Ha, SB and An, G (1988) Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene. Proceedings of the National Academy of Sciences of the USA 85: 8017–8021.CrossRefGoogle ScholarPubMed
Jefferson, RA, Kavanagh, TA and Bevan, MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal 6: 3901–3907.CrossRefGoogle ScholarPubMed
Kuhlemeier, C, Fluhr, R, Green, PJ and Chua, NH (1987) Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements. Genes & Development 1: 247–255.CrossRefGoogle ScholarPubMed
Lam, E and Chua, NH (1990) Gt1 binding site confers light-responsive expression in transgenic tobacco. Science 248: 471–474.CrossRefGoogle ScholarPubMed
Matsuoka, M, Kyozuka, J, Shimamoto, K, Kano-Murakami, Y (1994) The promoters of two carboxylases in a C4 plant (maize) direct cell-specific, light-regulated expression in a C3 plant (rice). Plant Journal 6: 311–319.CrossRefGoogle Scholar
Millar, AJ and Kay, SA (1996) Integration of circadian and phototransduction pathways in the network controlling CAB gene transcription in Arabidopsis. Proceedings of the National Academy of Sciences of the USA 93: 15491–15496.CrossRefGoogle Scholar
Puente, P, Wei, N and Deng, XW (1996) Combinatorial interplay of promoter elements constitutes the minimal determinants for light and developmental control of gene expression in Arabidopsis. Proceedings of the National Academy of Sciences of the USA 15: 3732–3743.Google ScholarPubMed
Terzaghi, WB and Cashmore, AR (1995) Light-regulated transcription. Annual Review of Plant Physiology and Plant Molecular Biology 46: 445–474.CrossRefGoogle Scholar
Tobin, EM and Kehoe, DM (1994) Phytochrome regulated gene expression. Seminars in Cell Biology 5: 335–346.CrossRefGoogle ScholarPubMed
von Arnim, AG and Deng, XW (1996) Light control of seedling development. Annual Review of Plant Physiology and Plant Molecular Biology 47: 215–243.CrossRefGoogle ScholarPubMed
Yadav, V, Kundu, S, Chattopadhyay, D, Negi, P, Wei, N, Deng, XW, et al. . (2002) Light regulated modulation of Z-box containing promoters by photoreceptors and downstream regulatory components, COP1 and HY5, in Arabidopsis. Plant Journal 31: 741–753.CrossRefGoogle ScholarPubMed